##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062443_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1073633
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02160980521277	32.0	32.0	32.0	32.0	32.0
2	30.714762865895516	32.0	32.0	32.0	32.0	32.0
3	30.73103472043054	32.0	32.0	32.0	32.0	32.0
4	30.764195958954318	32.0	32.0	32.0	32.0	32.0
5	30.594989162963508	32.0	32.0	32.0	32.0	32.0
6	34.21361768872604	36.0	36.0	36.0	32.0	36.0
7	34.03586234774825	36.0	36.0	36.0	32.0	36.0
8	34.02645037922642	36.0	36.0	36.0	32.0	36.0
9	34.16931390894281	36.0	36.0	36.0	32.0	36.0
10	33.875613920212956	36.0	36.0	36.0	32.0	36.0
11	34.217332179618175	36.0	36.0	36.0	32.0	36.0
12	33.97667266188726	36.0	36.0	36.0	32.0	36.0
13	34.10615359252184	36.0	36.0	36.0	32.0	36.0
14	34.0172992074573	36.0	36.0	36.0	32.0	36.0
15	33.975389169297145	36.0	36.0	36.0	32.0	36.0
16	33.962616648333274	36.0	36.0	36.0	32.0	36.0
17	33.879588276440835	36.0	36.0	36.0	32.0	36.0
18	33.90321459940222	36.0	36.0	36.0	32.0	36.0
19	33.876655244389845	36.0	36.0	36.0	32.0	36.0
20	33.85096490141417	36.0	36.0	36.0	32.0	36.0
21	33.79733950055559	36.0	36.0	36.0	32.0	36.0
22	33.7588356542692	36.0	36.0	36.0	32.0	36.0
23	33.72852362026875	36.0	36.0	36.0	27.0	36.0
24	33.71505067374047	36.0	36.0	36.0	27.0	36.0
25	33.23973648350973	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	44.0
5	86.0
6	172.0
7	42.0
8	141.0
9	176.0
10	120.0
11	43.0
12	71.0
13	59.0
14	292.0
15	373.0
16	495.0
17	687.0
18	914.0
19	1319.0
20	1954.0
21	2826.0
22	4155.0
23	5976.0
24	8054.0
25	10722.0
26	14120.0
27	18208.0
28	24077.0
29	31676.0
30	39859.0
31	54016.0
32	77086.0
33	110328.0
34	240882.0
35	424649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.04889454000102	17.45793104090691	11.598690308187438	24.894484110904628
2	16.132325381242104	20.58819146122005	37.35110786552856	25.928375292009285
3	19.172918323891423	24.82577017871015	28.393546091495576	27.607765405902857
4	12.30018258374632	15.753064798598949	35.6545999925476	36.29215262510713
5	14.307183872972452	37.10833809289104	33.134720794872536	15.449757239263976
6	33.46040164893736	35.11604806667313	17.53207302436879	13.891477260020723
7	29.695704446536247	31.023886967948776	20.93124691277482	18.349161672740152
8	28.474469150164854	32.38009211744275	19.964185871783183	19.181252860609213
9	27.394139176382186	14.642456944369348	18.99724242599391	38.96616145325456
10	16.16693769767858	27.02545950945873	31.268648922838217	25.538953870024468
11	36.68582554110455	21.626465355508394	22.072739072541392	19.61497003084566
12	24.432362362969144	24.34665833562592	28.78666784793364	22.43431145347129
13	29.64232497160643	19.77376527611229	25.592441809504805	24.99146794277647
14	23.71114505411298	20.092578605091823	25.485599401707965	30.710676939087232
15	25.191584902951142	27.182725237552223	23.622915036909056	24.00277482258758
16	25.341164228758124	26.05043897272178	24.58209946070131	24.026297337818786
17	23.314571642244765	26.1244426043316	26.06392402820595	24.49706172521769
18	24.535565243746067	24.689996036649337	27.471335649173522	23.30310307043107
19	24.69416493364792	26.00078335555949	26.035381559437106	23.269670151355484
20	25.07576478135916	24.073711439223125	26.620060835622596	24.23046294379512
21	26.2779624220857	24.627354214925976	24.801460741635985	24.293222621352342
22	24.939371553506003	25.048969129860538	25.58399185153679	24.42766746509667
23	23.54652301482342	24.819020687879938	25.891279977467352	25.743176319829292
24	23.740222872346877	26.13867923560511	25.851532212412327	24.26956567963569
25	24.549268730305425	25.081575995068604	25.58814450631485	24.78101076831112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	363.0
1	363.0
2	736.0
3	1109.0
4	1109.0
5	1109.0
6	2822.0
7	4535.0
8	4535.0
9	4535.0
10	4422.5
11	4310.0
12	4310.0
13	4310.0
14	3976.0
15	3642.0
16	3642.0
17	3642.0
18	5831.0
19	8020.0
20	8020.0
21	8020.0
22	12811.5
23	17603.0
24	17603.0
25	17603.0
26	25788.5
27	33974.0
28	33974.0
29	33974.0
30	41695.0
31	49416.0
32	49416.0
33	49416.0
34	58997.0
35	68578.0
36	68578.0
37	68578.0
38	76695.0
39	84812.0
40	84812.0
41	84812.0
42	99122.5
43	113433.0
44	113433.0
45	113433.0
46	135452.0
47	157471.0
48	157471.0
49	157471.0
50	151533.5
51	145596.0
52	145596.0
53	145596.0
54	129270.0
55	112944.0
56	112944.0
57	112944.0
58	103721.0
59	94498.0
60	94498.0
61	94498.0
62	83400.5
63	72303.0
64	72303.0
65	72303.0
66	59743.5
67	47184.0
68	47184.0
69	47184.0
70	35831.0
71	24478.0
72	24478.0
73	24478.0
74	19055.0
75	13632.0
76	13632.0
77	13632.0
78	11394.0
79	9156.0
80	9156.0
81	9156.0
82	6375.5
83	3595.0
84	3595.0
85	3595.0
86	2646.0
87	1697.0
88	1697.0
89	1697.0
90	1161.5
91	626.0
92	626.0
93	626.0
94	376.5
95	127.0
96	127.0
97	127.0
98	329.0
99	531.0
100	531.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00912788634477517
2	0.003446242803639605
3	0.004750226567178915
4	0.014250679701536744
5	0.032972160878065405
6	0.04256575570981891
7	0.0617529453733259
8	0.08121956012901987
9	0.0885777542232774
10	0.11037291141386303
11	0.11149061178261101
12	0.12462359111539978
13	0.11242202875656765
14	0.11614769665239426
15	0.10543640145189279
16	0.10515697635970578
17	0.11502999628364627
18	0.12182934019352983
19	0.1232264656544648
20	0.11502999628364627
21	0.12117734831176016
22	0.1424136553179718
23	0.13142293502528332
24	0.1266727084581044
25	0.1229470405622778
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1073633.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.78629827448991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52263252564597	39.37087538471129
2	13.50470612183	13.717080664251766
3	4.121858940622798	6.280018728115273
4	1.718037750595355	3.490111105942776
5	0.8288727884995785	2.1047690334173885
6	0.49282907328255093	1.5017378588440846
7	0.31436850603440136	1.1175928900898222
8	0.21979386284042696	0.8930013341692999
9	0.1608600188463497	0.7352536407813706
>10	0.9010427348508232	8.503949040292076
>50	0.09435612335356118	3.3514110550845
>100	0.10684869337557207	11.33959836957633
>500	0.009563049754480137	3.443787345232855
>1k	0.004229810468327753	4.150813549491173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4924	0.45862971797625446	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4397	0.409544043448739	No Hit
TCCATGTACTCTGCGTTGATACCAC	4058	0.3779690080316086	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3271	0.3046664921812202	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3225	0.30038197410101963	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2237	0.2083579770741026	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2096	0.19522499774131383	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1787	0.16644421324605335	No Hit
CATGTACTCTGCGTTGATACCACTG	1773	0.16514022948251403	No Hit
GTACATGGGGTGGTATCAACGCAAA	1545	0.14390392247630243	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1538	0.14325193059453276	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1329	0.12378531583883878	No Hit
CCCATGTACTCTGCGTTGATACCAC	1220	0.11363287082271131	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1187	0.11055919480865435	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1162	0.10823065237376274	No Hit
GCGTTGATACCACTGCTTCCATGTA	1152	0.10729923539980607	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1125	0.10478440957012312	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1097	0.1021764420430445	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1091	0.10161759185867052	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1087	0.10124502506908785	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1084	0.10096559997690085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.3141697395665E-5
2	0.0	0.0	0.0	0.0	9.3141697395665E-5
3	0.0	0.0	0.0	0.0	9.3141697395665E-5
4	0.0	0.0	0.0	0.0	9.3141697395665E-5
5	0.0	9.3141697395665E-5	0.0	0.0	9.3141697395665E-5
6	0.0	9.3141697395665E-5	0.0	0.0	1.8628339479133E-4
7	0.0	9.3141697395665E-5	0.0	0.0	1.8628339479133E-4
8	0.0	9.3141697395665E-5	0.0	0.0	1.8628339479133E-4
9	0.0	9.3141697395665E-5	0.0	9.3141697395665E-5	1.8628339479133E-4
10	0.0	9.3141697395665E-5	0.0	9.3141697395665E-5	1.8628339479133E-4
11	0.0	9.3141697395665E-5	0.0	9.3141697395665E-5	1.8628339479133E-4
12	0.0	9.3141697395665E-5	0.0	9.3141697395665E-5	3.7256678958266E-4
13	0.0	9.3141697395665E-5	0.0	9.3141697395665E-5	3.7256678958266E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCG	40	0.0052712504	14.252624	12
CGTGTCG	40	0.0052745123	14.251294	9
AAGGGCG	40	0.005276144	14.25063	5
TCGCGTA	110	1.8553692E-10	13.819437	9
AACCGCG	70	7.2542207E-6	13.572027	7
GCGTAAC	115	3.765308E-10	13.217976	11
CGCGTAA	120	7.366907E-10	12.668408	10
GTCGCGT	120	7.385097E-10	12.667227	8
TACAGTC	75	1.4769012E-5	12.667226	7
CTTATAC	60	4.106388E-4	12.660733	3
GTGTATA	205	0.0	12.505169	1
CCGTCGT	225	0.0	12.245556	9
TAGGAGT	70	1.0922179E-4	12.211978	4
CGTCGTA	230	0.0	11.979906	10
GGGTCGC	135	3.7107384E-10	11.963491	6
TATTAGC	120	1.0073563E-8	11.868885	2
ACCGTCG	225	0.0	11.822744	8
ATTAGCT	105	2.7332135E-7	11.756395	3
CGCCAGT	195	0.0	11.693914	18
CGAACGT	65	8.032586E-4	11.6901	4
>>END_MODULE