FastQCFastQC Report
Thu 2 Feb 2017
SRR4062443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073633
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20660.1924307468194439No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19820.18460684423820803No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12010.11186317857219366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.10701981030761909No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11450.10664724351803641No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11210.10441184278054047No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11080.10320100071439682No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11040.10282843392481415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT450.001398189924.44421232
GAACCGG602.872673E-421.999796
CGCAATC550.00448353819.9998144
AGATCGA808.988033E-519.24981938
GTATAGT708.1052445E-418.8631131
CGTCGTA2950.018.6438910
AACCGGG600.00741198318.3331587
ATACCGT3200.017.874836
ATACGAA3450.017.85490240
TAGGACA2350.017.7870644
TACGAAT3500.017.59983341
CGAATGC3550.017.35194843
GTATATA1651.6516424E-917.338821
TTAACGG902.2152427E-417.11094935
ACCGTCG3250.016.9229168
TACCGTC3150.016.7617457
TAGCGGC3550.016.73223730
TCCTATA1206.402939E-616.500612
CAATACG3750.016.4265138
TTGCGCG2151.2732926E-1116.37193718
Produced by FastQC (version 0.11.5)