FastQCFastQC Report
Thu 2 Feb 2017
SRR4062442_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062442_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844392
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26720.3164407052648533No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20380.24135709480904602No Hit
TATCAACGCAGAGTACTTTTTTTTT19020.22525083136742174No Hit
GTACATGGAAGCAGTGGTATCAACG16070.19031445110801617No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14040.16627348435323877No Hit
TCCATGTACTCTGCGTTGATACCAC13390.15857563785540366No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11250.1332319586163772No Hit
GGTATCAACGCAGAGTACTTTTTTT8700.10303271466333172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA250.0060178919.00652517
CTCGACG250.0060282119.00088913
TCGACGC350.00216755416.28744314
CCGTCGT508.698528E-515.2016129
CGTCTTA801.284352E-714.25151315
TTAGGCA852.7022725E-713.4084164
CGGTTCT751.4768735E-512.66650912
TCTCGTC905.3959593E-712.66650912
ACGGTAT1301.9645086E-1012.42439659
TATTATA1155.351467E-912.3845392
CGCATCG1001.4384932E-712.35057713
GTTTTCG550.003064006612.09219315
CGTCGTA550.003064006612.09219310
TAATATA1459.822543E-1111.7901584
AACGGAC658.009605E-411.69354915
GTCTTCG1302.6066118E-911.69216211
ATCGCCA1157.033486E-811.56781816
TATTAGC1104.9923983E-711.2211432
ATTAGCT855.335916E-511.1723553
CGACCTC600.005867243711.0845114
Produced by FastQC (version 0.11.5)