##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062442_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844392
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.147971558233618	32.0	32.0	32.0	32.0	32.0
2	30.90012695525301	32.0	32.0	32.0	32.0	32.0
3	30.903171749613925	32.0	32.0	32.0	32.0	32.0
4	30.929753005712985	32.0	32.0	32.0	32.0	32.0
5	30.811169456839952	32.0	32.0	32.0	32.0	32.0
6	34.43346099915679	36.0	36.0	36.0	32.0	36.0
7	34.326264341680165	36.0	36.0	36.0	32.0	36.0
8	34.31539024528892	36.0	36.0	36.0	32.0	36.0
9	34.40953135510521	36.0	36.0	36.0	32.0	36.0
10	34.16989620934353	36.0	36.0	36.0	32.0	36.0
11	34.43279187865352	36.0	36.0	36.0	32.0	36.0
12	34.2507839960587	36.0	36.0	36.0	32.0	36.0
13	34.34476404324058	36.0	36.0	36.0	32.0	36.0
14	34.27405873101593	36.0	36.0	36.0	32.0	36.0
15	34.233928080796595	36.0	36.0	36.0	32.0	36.0
16	34.2329214393315	36.0	36.0	36.0	32.0	36.0
17	34.16742223990753	36.0	36.0	36.0	32.0	36.0
18	34.18552639058636	36.0	36.0	36.0	32.0	36.0
19	34.168209788818466	36.0	36.0	36.0	32.0	36.0
20	34.13947431998408	36.0	36.0	36.0	32.0	36.0
21	34.10296047333466	36.0	36.0	36.0	32.0	36.0
22	34.07657225554008	36.0	36.0	36.0	32.0	36.0
23	34.04937754028934	36.0	36.0	36.0	32.0	36.0
24	34.02820490956807	36.0	36.0	36.0	32.0	36.0
25	33.58933883788572	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	33.0
5	81.0
6	128.0
7	38.0
8	122.0
9	166.0
10	114.0
11	31.0
12	64.0
13	61.0
14	184.0
15	223.0
16	337.0
17	417.0
18	572.0
19	822.0
20	1191.0
21	1723.0
22	2397.0
23	3651.0
24	4744.0
25	6820.0
26	9236.0
27	11771.0
28	15958.0
29	21602.0
30	27823.0
31	38045.0
32	54083.0
33	79730.0
34	184371.0
35	377848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.132288479964565	17.68268056646165	11.303887465668003	25.881143487905785
2	16.298500887056086	20.71163452815485	37.406645933161684	25.583218651627377
3	19.02711306763877	24.618968606732018	28.584016400723396	27.769901924905817
4	12.699995735951145	15.944936915766084	35.85650528031383	35.49856206796893
5	14.310033952759934	36.69753137609375	33.88120561914623	15.111229052000086
6	33.249171214422645	35.38280533550709	17.519306734786248	13.848716715284015
7	29.291988414700626	31.03191645690828	21.297468204420806	18.378626923970288
8	27.59679723106283	32.97939299112779	20.21798268228935	19.205827095520036
9	27.555131957509005	14.620178361647623	19.095223023186065	38.729466657657305
10	15.866714570119866	27.244021850614743	32.150212897649055	24.73905068161634
11	36.196889336307166	21.678956835598683	22.764499494282965	19.359654333811186
12	24.412494708094332	24.470957665940524	29.13862599004588	21.977921635919262
13	29.485533101160982	20.185121285746302	25.390975633760565	24.938369979332155
14	23.255488543843132	20.19703498865175	25.9549959563715	30.592480511133612
15	24.740484634200417	27.494934188918883	23.335811401246623	24.42876977563407
16	24.91896108946648	26.164838005174158	24.746686711989966	24.169514193369395
17	23.294199394552393	26.184008167495314	26.15400851136489	24.367783926587403
18	24.066150905270348	25.281044108509114	27.34511742188871	23.30768756433183
19	24.83676794603915	25.63259091307967	26.1319517407071	23.39868940017408
20	24.98268771966438	24.940711368713625	26.26545654617636	23.81114436544564
21	25.950008063980572	24.776224539167227	25.142068363581167	24.131699033271033
22	25.019304194970587	25.337185869447893	25.488416889954816	24.155093045626696
23	23.932364926729065	25.16947906239771	26.05364030114852	24.844515709724707
24	23.910411263343022	25.87630198605127	25.972598255993912	24.2406884946118
25	24.261825788632965	25.24977379536396	26.197639407871563	24.290761008131508
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	221.0
1	221.0
2	513.0
3	805.0
4	805.0
5	805.0
6	1984.5
7	3164.0
8	3164.0
9	3164.0
10	3152.5
11	3141.0
12	3141.0
13	3141.0
14	3117.0
15	3093.0
16	3093.0
17	3093.0
18	5094.0
19	7095.0
20	7095.0
21	7095.0
22	11016.0
23	14937.0
24	14937.0
25	14937.0
26	22002.0
27	29067.0
28	29067.0
29	29067.0
30	35685.0
31	42303.0
32	42303.0
33	42303.0
34	49576.5
35	56850.0
36	56850.0
37	56850.0
38	63638.5
39	70427.0
40	70427.0
41	70427.0
42	79610.5
43	88794.0
44	88794.0
45	88794.0
46	101279.0
47	113764.0
48	113764.0
49	113764.0
50	112363.5
51	110963.0
52	110963.0
53	110963.0
54	101854.5
55	92746.0
56	92746.0
57	92746.0
58	84004.5
59	75263.0
60	75263.0
61	75263.0
62	65930.5
63	56598.0
64	56598.0
65	56598.0
66	46218.0
67	35838.0
68	35838.0
69	35838.0
70	27376.0
71	18914.0
72	18914.0
73	18914.0
74	14505.0
75	10096.0
76	10096.0
77	10096.0
78	8039.0
79	5982.0
80	5982.0
81	5982.0
82	4227.5
83	2473.0
84	2473.0
85	2473.0
86	1778.0
87	1083.0
88	1083.0
89	1083.0
90	723.0
91	363.0
92	363.0
93	363.0
94	221.0
95	79.0
96	79.0
97	79.0
98	206.0
99	333.0
100	333.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007460989682517125
2	0.0030791385991340518
3	0.004618707898701077
4	0.014685122549716245
5	0.03292309732920255
6	0.04571336535637476
7	0.06679362191967711
8	0.08846602052127447
9	0.09770343631867663
10	0.12186283148111303
11	0.12257340192706706
12	0.13299510180105922
13	0.12470511326492907
14	0.12908696434831216
15	0.11759940880538895
16	0.11487555542923193
17	0.12423139963429308
18	0.1312186756861742
19	0.1319292461321282
20	0.1255341121185421
21	0.13453467110062625
22	0.1552596424409516
23	0.1444826573439824
24	0.1374953812921013
25	0.13370567224701324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	844392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.48760856795145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.36127117404376	52.09046482850501
2	11.08199356474048	13.8497455205212
3	2.5659569704002663	4.810215443057351
4	0.960363998079613	2.4004339837900694
5	0.48648206851142534	1.5199550536234643
6	0.29445707182661324	1.1039950946619943
7	0.18713181833498624	0.8185393870307934
8	0.13558023566248756	0.6777667756502507
9	0.10654698566752603	0.5992079701038749
>10	0.6599095641328789	8.003252603073653
>50	0.08880704221428841	3.985273846070584
>100	0.06769634116625452	7.8034983877272355
>500	0.0024720573926836843	0.901409103416659
>1k	0.0013311078268296761	1.4362420027678826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2672	0.3164407052648533	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2038	0.24135709480904602	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1902	0.22525083136742174	No Hit
GTACATGGAAGCAGTGGTATCAACG	1607	0.19031445110801617	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1404	0.16627348435323877	No Hit
TCCATGTACTCTGCGTTGATACCAC	1339	0.15857563785540366	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1125	0.1332319586163772	No Hit
GGTATCAACGCAGAGTACTTTTTTT	870	0.10303271466333172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.1842840765900199E-4	0.0
12	0.0	0.0	0.0	1.1842840765900199E-4	0.0
13	0.0	0.0	0.0	1.1842840765900199E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	25	0.00601789	19.006525	17
CTCGACG	25	0.00602821	19.000889	13
TCGACGC	35	0.002167554	16.287443	14
CCGTCGT	50	8.698528E-5	15.201612	9
CGTCTTA	80	1.284352E-7	14.251513	15
TTAGGCA	85	2.7022725E-7	13.408416	4
CGGTTCT	75	1.4768735E-5	12.666509	12
TCTCGTC	90	5.3959593E-7	12.666509	12
ACGGTAT	130	1.9645086E-10	12.4243965	9
TATTATA	115	5.351467E-9	12.384539	2
CGCATCG	100	1.4384932E-7	12.350577	13
GTTTTCG	55	0.0030640066	12.092193	15
CGTCGTA	55	0.0030640066	12.092193	10
TAATATA	145	9.822543E-11	11.790158	4
AACGGAC	65	8.009605E-4	11.693549	15
GTCTTCG	130	2.6066118E-9	11.692162	11
ATCGCCA	115	7.033486E-8	11.567818	16
TATTAGC	110	4.9923983E-7	11.221143	2
ATTAGCT	85	5.335916E-5	11.172355	3
CGACCTC	60	0.0058672437	11.08451	14
>>END_MODULE