FastQCFastQC Report
Thu 2 Feb 2017
SRR4062442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844392
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAT300.005744235629.33313430
CTAGACC500.002579900221.9998534
ACTATAC654.928724E-420.3087583
GTCGATA550.004482686519.9998659
GTCGTAA901.0014317E-519.55542430
CCGAGTA600.007410587718.33320814
GTATACT851.4302594E-418.1175254
GTATTAT1002.4779327E-517.603011
GTCTAGT750.001288955617.6030081
GGGCCGT750.001290491817.5998826
ATACCGT1651.6570993E-917.3332166
TAGGACG2201.8189894E-1216.9998844
CGGCAAA1053.7723883E-516.76179136
TATACTC953.3449894E-416.2104175
ACCGTCG1805.2586984E-915.8887828
ATTAGAT1552.662855E-715.6137232
CCTACAC1552.662855E-715.6137233
TACTATA850.002977903215.5302272
TACCGTC1704.4845365E-815.5293067
GTATAGA1004.9350824E-415.4026341
Produced by FastQC (version 0.11.5)