##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062442_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 844392 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.232829065173522 32.0 32.0 32.0 32.0 32.0 2 31.42947706752314 32.0 32.0 32.0 32.0 32.0 3 31.510350642829398 32.0 32.0 32.0 32.0 32.0 4 31.61208064500848 32.0 32.0 32.0 32.0 32.0 5 31.55202796805275 32.0 32.0 32.0 32.0 32.0 6 35.13817634463614 36.0 36.0 36.0 36.0 36.0 7 35.15025130508105 36.0 36.0 36.0 36.0 36.0 8 35.10485414357313 36.0 36.0 36.0 36.0 36.0 9 35.21673582885674 36.0 36.0 36.0 36.0 36.0 10 35.09564515059356 36.0 36.0 36.0 36.0 36.0 11 35.21240608627273 36.0 36.0 36.0 36.0 36.0 12 35.14200868790799 36.0 36.0 36.0 36.0 36.0 13 35.170255047418735 36.0 36.0 36.0 36.0 36.0 14 35.14338719457314 36.0 36.0 36.0 36.0 36.0 15 35.101780926394376 36.0 36.0 36.0 36.0 36.0 16 35.120211939478345 36.0 36.0 36.0 36.0 36.0 17 35.089959402741854 36.0 36.0 36.0 36.0 36.0 18 35.1003633383547 36.0 36.0 36.0 36.0 36.0 19 35.07807748060143 36.0 36.0 36.0 36.0 36.0 20 35.054749452860754 36.0 36.0 36.0 36.0 36.0 21 35.04924134762054 36.0 36.0 36.0 36.0 36.0 22 35.03817539720888 36.0 36.0 36.0 36.0 36.0 23 35.00643303110404 36.0 36.0 36.0 36.0 36.0 24 34.99259940880539 36.0 36.0 36.0 36.0 36.0 25 34.96630830230509 36.0 36.0 36.0 36.0 36.0 26 34.9226544069579 36.0 36.0 36.0 32.0 36.0 27 34.90589560299008 36.0 36.0 36.0 32.0 36.0 28 34.89216382912202 36.0 36.0 36.0 32.0 36.0 29 34.867246492150564 36.0 36.0 36.0 32.0 36.0 30 34.844530739277495 36.0 36.0 36.0 32.0 36.0 31 34.842070981250416 36.0 36.0 36.0 32.0 36.0 32 34.79682303953614 36.0 36.0 36.0 32.0 36.0 33 34.76312423613677 36.0 36.0 36.0 32.0 36.0 34 34.74353262465774 36.0 36.0 36.0 32.0 36.0 35 34.707295900482244 36.0 36.0 36.0 32.0 36.0 36 34.6785177974211 36.0 36.0 36.0 32.0 36.0 37 34.66932183156638 36.0 36.0 36.0 32.0 36.0 38 34.63054126519437 36.0 36.0 36.0 32.0 36.0 39 34.61324479625577 36.0 36.0 36.0 32.0 36.0 40 34.58917659096723 36.0 36.0 36.0 32.0 36.0 41 34.56313655269117 36.0 36.0 36.0 32.0 36.0 42 34.52091800964481 36.0 36.0 36.0 32.0 36.0 43 34.499481283574454 36.0 36.0 36.0 32.0 36.0 44 34.47217524562052 36.0 36.0 36.0 32.0 36.0 45 34.40767913480943 36.0 36.0 36.0 32.0 36.0 46 34.409621360695034 36.0 36.0 36.0 32.0 36.0 47 34.35081099773565 36.0 36.0 36.0 32.0 36.0 48 34.31359723919696 36.0 36.0 36.0 32.0 36.0 49 34.2930356990592 36.0 36.0 36.0 32.0 36.0 50 33.76390467934324 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 6.0 21 21.0 22 71.0 23 240.0 24 620.0 25 1553.0 26 2972.0 27 5616.0 28 9522.0 29 14015.0 30 19811.0 31 27498.0 32 40047.0 33 63471.0 34 136947.0 35 521982.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37747834501808 18.33717692235713 11.976007476343273 26.309337256281513 2 15.677895843893749 21.25628295442164 37.22386977881158 25.841951422873034 3 18.396347637629752 24.98599572469904 28.86965069251585 27.748005945155352 4 12.398062506661613 16.431625196887694 36.05407453901633 35.11623775743436 5 13.986039659305158 37.07578944376546 33.66635401567045 15.27181688125894 6 33.31924035282191 35.71492861135586 17.201726212470035 13.764104823352188 7 29.188255204046463 31.090691397705566 21.402525607212773 18.318527791035198 8 27.708220826346057 33.08522581928772 20.065561966480022 19.140991387886196 9 27.571317587092253 14.369629271712665 19.128793261897318 38.930259879297765 10 15.830798965409432 27.307340666420338 32.306795895745104 24.55506447242513 11 36.32211105742357 21.505059261575195 22.81333788098419 19.359491800017054 12 24.43876777610399 24.327445072904528 29.196629053804397 22.03715809718709 13 29.566244114108137 20.17498981515694 25.482832618025753 24.775933452709168 14 23.257207552890126 20.12169703171039 26.08847549479389 30.532619920605597 15 24.929298240627578 27.412978806052163 23.239680148556594 24.418042804763665 16 24.948246785853016 26.05721039517191 24.80885655003837 24.185686268936703 17 23.40157178182645 25.874830647377046 26.207022330860546 24.516575239935953 18 24.094496395039272 25.041094657457673 27.42908506949379 23.435323878009264 19 24.940075225724545 25.50568930070394 26.33954371903097 23.214691754540546 20 25.229869539266126 24.637609072563453 26.39567878426134 23.736842603909082 21 25.94766411808733 24.644477920207674 25.3157301348189 24.092127826886088 22 25.024514680385412 25.109783133899892 25.707728164170195 24.157974021544497 23 23.96280400572246 24.811461975006868 26.289211645775897 24.936522373494775 24 24.199423964225147 25.48449061573298 26.099015623075537 24.217069796966335 25 24.373632151891538 24.822475816919155 26.405271485281716 24.398620545907587 26 23.62362504618708 25.56573250338705 26.822021051833744 23.988621398592123 27 24.619963239822262 25.41497314043714 25.991364200513505 23.97369941922709 28 23.86936399207951 25.111796416830096 26.462235549365698 24.556604041724697 29 23.8936418156496 25.36215407062123 26.397218353560902 24.346985760168263 30 24.09248311210907 25.368786061450134 26.619034761106214 23.919696065334584 31 24.183436129191183 25.24325194933159 25.918767586618536 24.65454433485869 32 23.800438658821967 25.549981525168402 26.06443452803911 24.585145287970516 33 23.788832674871387 25.1374953812921 26.3458204246369 24.727851519199614 34 24.137130621796512 25.321059413163553 26.713422202010438 23.828387763029493 35 24.690664999194688 25.169115766137057 26.272039526665342 23.868179708002916 36 23.86344257169656 25.524282560706403 26.146623842954458 24.465651024642582 37 24.580881865294792 25.349837516224692 25.839183696671686 24.230096921808826 38 23.83608560952733 25.43178997432472 26.13312300448133 24.599001411666617 39 24.039782470700814 25.12813953708704 26.045841268036646 24.7862367241755 40 24.464585168973652 25.125415683710884 26.45252441993766 23.957474727377807 41 23.634638888099367 25.133705672247014 26.91439520980777 24.317260229845854 42 24.428701361452976 25.648395531933037 26.385257084387348 23.537646022226642 43 23.672891263773224 24.788368435513362 26.54999099944102 24.988749301272396 44 23.281485376460225 25.175037186520004 26.619508474736854 24.92396896228292 45 23.35183185060967 25.349719087817036 26.654800140219237 24.643648921354064 46 23.258419381542435 24.93418333449788 26.745429546264702 25.061967737694978 47 23.481629385403934 24.9644714777023 26.980951974912127 24.572947161981638 48 23.484945380818388 25.873291078077482 26.223247022709835 24.418516518394302 49 22.808956029900802 25.56975906924746 26.695421084046274 24.925863816805467 50 22.67077692680287 26.22280436432885 26.21534336581039 24.891075343057896 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 48.0 1 49.5 2 51.0 3 157.5 4 264.0 5 293.5 6 323.0 7 277.0 8 231.0 9 326.5 10 422.0 11 624.5 12 827.0 13 1365.5 14 1904.0 15 2530.0 16 3156.0 17 3543.5 18 3931.0 19 4281.0 20 4631.0 21 5056.0 22 5481.0 23 5683.0 24 5885.0 25 6558.5 26 7232.0 27 8716.0 28 10200.0 29 11937.5 30 13675.0 31 15161.0 32 16647.0 33 19338.0 34 22029.0 35 25041.0 36 28053.0 37 31974.5 38 35896.0 39 37954.0 40 40012.0 41 42877.0 42 45742.0 43 47109.0 44 48476.0 45 52214.0 46 55952.0 47 58664.0 48 61376.0 49 61539.5 50 61703.0 51 59879.0 52 58055.0 53 56470.5 54 54886.0 55 54585.0 56 54284.0 57 52377.5 58 50471.0 59 46244.0 60 42017.0 61 36884.0 62 31751.0 63 27791.0 64 23831.0 65 20166.0 66 16501.0 67 14377.0 68 12253.0 69 11016.0 70 9779.0 71 7747.0 72 5715.0 73 4965.5 74 4216.0 75 3197.0 76 2178.0 77 1875.5 78 1573.0 79 1364.5 80 1156.0 81 907.0 82 658.0 83 574.5 84 491.0 85 376.0 86 261.0 87 188.5 88 116.0 89 74.5 90 33.0 91 22.0 92 11.0 93 6.5 94 2.0 95 3.0 96 4.0 97 3.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015277264588011255 2 0.0052108499369960875 3 8.289988536130139E-4 4 2.3685681531800394E-4 5 0.0 6 0.0 7 1.1842840765900197E-4 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 1.1842840765900197E-4 47 0.0 48 0.0 49 0.0 50 1.1842840765900197E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 844392.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.5726440783211 #Duplication Level Percentage of deduplicated Percentage of total 1 84.71827986366884 57.24638172155293 2 10.312381985944738 13.936698350718679 3 2.3581356409994427 4.780363810729768 4 0.890956055080758 2.408170255975885 5 0.42639608928190764 1.4406355588717188 6 0.25337035034595806 1.027254270235617 7 0.17043321147700868 0.80616359167828 8 0.12607304619826462 0.6815271262900057 9 0.0890867875129046 0.5417846806221471 >10 0.5286191683294758 6.933303172738718 >50 0.07442257184617442 3.5814488988754936 >100 0.05061081910830319 6.119726689709923 >500 0.00123441020615603 0.4965418720008868 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 1.1842840765900199E-4 4 0.0 0.0 0.0 0.0 1.1842840765900199E-4 5 0.0 0.0 0.0 0.0 1.1842840765900199E-4 6 0.0 0.0 0.0 0.0 1.1842840765900199E-4 7 0.0 0.0 0.0 0.0 1.1842840765900199E-4 8 0.0 0.0 0.0 0.0 1.1842840765900199E-4 9 0.0 0.0 0.0 0.0 1.1842840765900199E-4 10 0.0 0.0 0.0 1.1842840765900199E-4 1.1842840765900199E-4 11 0.0 0.0 0.0 2.3685681531800397E-4 1.1842840765900199E-4 12 0.0 0.0 0.0 2.3685681531800397E-4 1.1842840765900199E-4 13 0.0 0.0 0.0 2.3685681531800397E-4 1.1842840765900199E-4 14 0.0 0.0 0.0 2.3685681531800397E-4 1.1842840765900199E-4 15 0.0 0.0 0.0 2.3685681531800397E-4 1.1842840765900199E-4 16 0.0 0.0 0.0 2.3685681531800397E-4 1.1842840765900199E-4 17 0.0 0.0 0.0 2.3685681531800397E-4 1.1842840765900199E-4 18 0.0 0.0 0.0 7.105704459540119E-4 1.1842840765900199E-4 19 0.0 0.0 0.0 7.105704459540119E-4 1.1842840765900199E-4 20 0.0 0.0 0.0 8.289988536130139E-4 1.1842840765900199E-4 21 0.0 0.0 0.0 0.0010658556689310179 1.1842840765900199E-4 22 0.0 0.0 0.0 0.0015395692995670257 1.1842840765900199E-4 23 0.0 0.0 0.0 0.0034344238221110574 1.1842840765900199E-4 24 0.0 0.0 0.0 0.006158277198268103 1.1842840765900199E-4 25 0.0 0.0 0.0 0.008053131720812136 1.1842840765900199E-4 26 0.0 0.0 0.0 0.010421699873992175 1.1842840765900199E-4 27 0.0 0.0 0.0 0.01432983732673924 1.1842840765900199E-4 28 0.0 0.0 0.0 0.02510682242370842 1.1842840765900199E-4 29 0.0 0.0 0.0 0.05033207325507584 1.1842840765900199E-4 30 0.0 0.0 0.0 0.09711129428038162 1.1842840765900199E-4 31 0.0 0.0 0.0 0.18913016703142616 1.1842840765900199E-4 32 0.0 0.0 0.0 0.3096902860282902 1.1842840765900199E-4 33 0.0 0.0 0.0 0.42681598120304315 1.1842840765900199E-4 34 0.0 0.0 0.0 0.5625349363802594 1.1842840765900199E-4 35 0.0 0.0 0.0 0.7149522970373949 1.1842840765900199E-4 36 0.0 0.0 0.0 0.9357028489137746 1.1842840765900199E-4 37 0.0 0.0 0.0 1.2696709585121602 1.1842840765900199E-4 38 0.0 0.0 0.0 1.7058427839202646 1.1842840765900199E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCAAT 30 0.0057442356 29.333134 30 CTAGACC 50 0.0025799002 21.999853 4 ACTATAC 65 4.928724E-4 20.308758 3 GTCGATA 55 0.0044826865 19.999865 9 GTCGTAA 90 1.0014317E-5 19.555424 30 CCGAGTA 60 0.0074105877 18.333208 14 GTATACT 85 1.4302594E-4 18.117525 4 GTATTAT 100 2.4779327E-5 17.60301 1 GTCTAGT 75 0.0012889556 17.603008 1 GGGCCGT 75 0.0012904918 17.599882 6 ATACCGT 165 1.6570993E-9 17.333216 6 TAGGACG 220 1.8189894E-12 16.999884 4 CGGCAAA 105 3.7723883E-5 16.761791 36 TATACTC 95 3.3449894E-4 16.210417 5 ACCGTCG 180 5.2586984E-9 15.888782 8 ATTAGAT 155 2.662855E-7 15.613723 2 CCTACAC 155 2.662855E-7 15.613723 3 TACTATA 85 0.0029779032 15.530227 2 TACCGTC 170 4.4845365E-8 15.529306 7 GTATAGA 100 4.9350824E-4 15.402634 1 >>END_MODULE