FastQCFastQC Report
Thu 2 Feb 2017
SRR4062441_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062441_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308350
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT142491.0890816677494555No Hit
GTCCTACAGTGGACATTTCTAAATT136221.0411587113539955No Hit
CTGTAGGACGTGGAATATGGCAAGA126970.970458975044904No Hit
CTTTAGGACGTGAAATATGGCGAGG112430.859326632781748No Hit
GTCCTACAGTGTGCATTTCTCATTT80210.6130622539840257No Hit
CTGAAGGACCTGGAATATGGCGAGA60530.4626437879772232No Hit
CTGTAGGACCTGGAATATGGCGAGA57560.43994344021095266No Hit
GTACATGGAAGCAGTGGTATCAACG56430.43130660755913935No Hit
GTCCTTCAGTGTGCATTTCTCATTT56090.42870791454885926No Hit
ATTTAGAAATGTCCACTGTAGGACG50210.38376581190048537No Hit
TCCATGTACTCTGCGTTGATACCAC45180.34532044177781174No Hit
GTATCAACGCAGAGTACTTTTTTTT43760.3344670768525242No Hit
TTTCTAAATTTTCCACCTTTTTCAG39670.30320632858180147No Hit
GAATATGGCAAGAAAACTGAAAATC37250.2847097489203959No Hit
ATTCCAGGTCCTTCAGTGTGCATTT31990.24450643940841518No Hit
GGAATATGGCGAGAAAACTGAAAAT31850.24343638934535863No Hit
TATCAACGCAGAGTACTTTTTTTTT30530.23334734589368286No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29050.22203538808422824No Hit
CTGTAGGACATGGAATATGGCAAGA28950.22127106661061643No Hit
CCATATTCCAGGTCCTTCAGTGTGC27920.21339855543241487No Hit
TTCCAGGTCCTTCAGTGTGCATTTC26900.20560247640157447No Hit
ACCTGGAATATGGCGAGAAAACTGA26620.20346237627546143No Hit
GCCATATTCCACGTCCTACAGTGGA26180.2000993617915695No Hit
GAAATATGGCGAGGAAAACTGAAAA25590.1955898650972599No Hit
GACCTGGAATATGGCGAGAAAACTG25530.19513127221309284No Hit
GATATACACTGTTCTACAAATCCCG25400.1941376542973975No Hit
GAGTACATGGAAGCAGTGGTATCAA25070.19161539343447853No Hit
CCATATTTCACGTCCTAAAGTGTGT24290.1856536859403065No Hit
ACAGTGGACATTTCTAAATTTTCCA24170.18473650017197232No Hit
GTCCACTGTAGGACGTGGAATATGG23530.1798448427408568No Hit
ATACACACTTTAGGACGTGAAATAT23320.17823976764627203No Hit
TTGTAGAACAGTGTATATCAATGAG22810.17434172813085183No Hit
CATGTACTCTGCGTTGATACCACTG22030.16838002063667978No Hit
CCTAAAGTGTGTATTTCTCATTTTC21450.16394695608973134No Hit
CCACTGTAGGACGTGGAATATGGCA21160.16173042381625713No Hit
CACTTTAGGACGTGAAATATGGCGA20680.15806168074292046No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20240.15469866625902856No Hit
GTGTATTTCTCATTTTCCGTGATTT20120.15378148049069437No Hit
GAAATACACACTTTAGGACGTGAAA19410.1483547980280506No Hit
CTACAGTGGACATTTCTAAATTTTC19370.14804906943860588No Hit
ATTCCACGTCCTACAGTGGACATTT19240.14705545152291052No Hit
AGTGTGTATTTCTCATTTTCCGTGA19120.14613826575457636No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18830.14392173348110215No Hit
GTGTATATCAATGAGTTACAATGAG18200.1391065081973478No Hit
GTAGGACGTGGAATATGGCAAGAAA17030.13016394695608974No Hit
GTTCTACAGTGTGGTTTTTATCATT16090.12297932510413881No Hit
GTGTATATCAATGAGTTACAATGAA15900.12152711430427637No Hit
TCCTAAAGTGTGTATTTCTCATTTT15870.12129781786219285No Hit
CCCATGTACTCTGCGTTGATACCAC15340.1172469140520503No Hit
ATATTTCACGTCCTAAAGTGTGTAT15130.1156418389574655No Hit
TTTTTCAAGTCGTCAAGTGGATGTT15000.11464822104177017No Hit
GTCCTACAGTGGACATTTCTAAATA14960.11434249245232544No Hit
GTACATGGGAAGCAGTGGTATCAAC14870.11365460312607484No Hit
GGTATCAACGCAGAGTACTTTTTTT14650.11197309588412888No Hit
GGTCCTTCAGTGTGCATTTCTCATT14490.11075018152634998No Hit
ACGTGGAATATGGCAAGAAAACTGA14440.11036802078954408No Hit
ACGTCCTACAGTGGACATTTCTAAA14280.10914510643176518No Hit
GTATCAACGCAGAGTACATGGGAAA13930.1064699812741239No Hit
GCTTCCATGTACTCTGCGTTGATAC13760.10517063476898385No Hit
GCGTTGATACCACTGCTTCCATGTA13500.10318339893759315No Hit
CCTTCAGTGTGCATTTCTCATTTTT13220.10104329881148011No Hit
GTTCTACAAATCCCGTTTCCAACGA13130.10035540948522949No Hit
CACTGAAGGACCTGGAATATGGCGA13100.10012611304314595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCG250.006046748718.9917353
CGGACCA456.758109E-414.77870117
GTACTAG653.391764E-614.6067921
GTGTCCG400.005270964514.2530729
TCCAACG3200.013.95399618
TAGAAAT13100.013.9208044
TAGGACC18750.013.8798164
CTAGGAC2950.013.84143453
CCAACGT1257.2759576E-1213.68085419
GTCCTAG3200.013.6482221
ATAATAC852.7122223E-713.4059323
CCAACGA2000.013.30083119
GGCGAAG801.997305E-613.06331519
GTTCTAG1102.7685019E-912.9469311
CCACCTT12200.012.9275313
AAATGTC14000.012.7594047
GGCGAGC604.0890966E-412.66745819
CTTATAC604.106292E-412.6611583
CACCTTT12800.012.61894114
CTAGAGT3200.012.4661884
Produced by FastQC (version 0.11.5)