##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062441_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1308350
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237509076317497	32.0	32.0	32.0	32.0	32.0
2	31.0345083502121	32.0	32.0	32.0	32.0	32.0
3	31.005295983490655	32.0	32.0	32.0	32.0	32.0
4	31.04668934153705	32.0	32.0	32.0	32.0	32.0
5	30.985699545228723	32.0	32.0	32.0	32.0	32.0
6	34.625427446784116	36.0	36.0	36.0	32.0	36.0
7	34.4996552910154	36.0	36.0	36.0	32.0	36.0
8	34.52282722513089	36.0	36.0	36.0	32.0	36.0
9	34.57782168380021	36.0	36.0	36.0	32.0	36.0
10	34.362033095119806	36.0	36.0	36.0	32.0	36.0
11	34.59774983758169	36.0	36.0	36.0	32.0	36.0
12	34.443082508503075	36.0	36.0	36.0	32.0	36.0
13	34.50024993312187	36.0	36.0	36.0	32.0	36.0
14	34.43956127947415	36.0	36.0	36.0	32.0	36.0
15	34.40721519471089	36.0	36.0	36.0	32.0	36.0
16	34.43870906103107	36.0	36.0	36.0	32.0	36.0
17	34.42780754385294	36.0	36.0	36.0	32.0	36.0
18	34.46949516566668	36.0	36.0	36.0	32.0	36.0
19	34.35429357587802	36.0	36.0	36.0	32.0	36.0
20	34.32930484961975	36.0	36.0	36.0	32.0	36.0
21	34.255997248442696	36.0	36.0	36.0	32.0	36.0
22	34.213325944892425	36.0	36.0	36.0	32.0	36.0
23	34.24608858485879	36.0	36.0	36.0	32.0	36.0
24	34.200022929644206	36.0	36.0	36.0	32.0	36.0
25	33.84517216341193	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	49.0
5	112.0
6	248.0
7	50.0
8	160.0
9	246.0
10	143.0
11	41.0
12	103.0
13	73.0
14	243.0
15	260.0
16	431.0
17	523.0
18	733.0
19	1014.0
20	1408.0
21	2101.0
22	3070.0
23	4460.0
24	6128.0
25	8628.0
26	11885.0
27	15804.0
28	21596.0
29	29458.0
30	38807.0
31	54076.0
32	78343.0
33	116255.0
34	279386.0
35	632514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.031048560624534	20.891334241932487	12.720897755077237	25.356719442365744
2	10.480241534816173	20.92631659405335	49.91095314530307	18.682488725827408
3	19.004865908524586	27.232730458019116	30.335568828222577	23.426834805233717
4	10.331392211151694	17.828460652908092	40.384336076588966	31.45581105935125
5	9.380325820764174	41.76231002900075	37.305689974057586	11.551674176177482
6	28.24676143962525	39.6764077730037	19.99125219746482	12.085578589906229
7	25.690160881386788	32.77911410073538	23.386348600557557	18.144376417320277
8	21.49364274693306	45.299855344858315	20.373731972611147	12.83276993559748
9	26.963168335617	15.325271266589091	22.11039783216243	35.60116256563148
10	15.42315776908344	29.83640853255875	36.47395690257435	18.26647679578346
11	32.07862348450762	20.261302871974753	30.7699665451518	16.890107098365824
12	26.646748517809627	23.80366745977021	35.0234678548169	14.526116167603265
13	31.029619613667858	24.514176964699345	26.48279809581092	17.973405325821872
14	17.848711612968888	27.375345216404483	27.82867012195542	26.94727304867121
15	18.541501837163498	42.5955654040918	22.918633780853405	15.944298977891295
16	16.31534689336605	28.32280866060819	37.913995327978796	17.447849118046964
17	16.823923916570248	33.53535724561822	33.04835717827692	16.59236165953461
18	17.235172572128263	26.581923930511977	41.58161781587204	14.601285681487717
19	23.662297131933215	26.214223780687057	28.47916773488003	21.644311352499695
20	23.388648186210517	29.547023236523412	32.21953716726532	14.844791410000749
21	19.219484421370662	27.69325780615064	27.224069927436968	25.86318784504173
22	21.786200878710407	35.60652450132419	27.587679684031656	15.019594935933744
23	16.278745825938078	34.540453289922326	32.1497098151588	17.031091068980796
24	22.93387496833532	28.23869389229494	33.32654758961965	15.500883549750089
25	19.193946833521085	31.649177156291326	33.028006685605135	16.128869324582457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	231.0
2	599.0
3	967.0
4	967.0
5	967.0
6	2778.5
7	4590.0
8	4590.0
9	4590.0
10	4697.5
11	4805.0
12	4805.0
13	4805.0
14	5417.0
15	6029.0
16	6029.0
17	6029.0
18	10821.5
19	15614.0
20	15614.0
21	15614.0
22	30193.0
23	44772.0
24	44772.0
25	44772.0
26	74640.0
27	104508.0
28	104508.0
29	104508.0
30	144035.5
31	183563.0
32	183563.0
33	183563.0
34	190846.5
35	198130.0
36	198130.0
37	198130.0
38	192646.5
39	187163.0
40	187163.0
41	187163.0
42	180704.5
43	174246.0
44	174246.0
45	174246.0
46	179718.5
47	185191.0
48	185191.0
49	185191.0
50	149655.5
51	114120.0
52	114120.0
53	114120.0
54	78563.0
55	43006.0
56	43006.0
57	43006.0
58	31578.5
59	20151.0
60	20151.0
61	20151.0
62	15130.0
63	10109.0
64	10109.0
65	10109.0
66	7752.5
67	5396.0
68	5396.0
69	5396.0
70	4040.0
71	2684.0
72	2684.0
73	2684.0
74	2077.0
75	1470.0
76	1470.0
77	1470.0
78	1077.5
79	685.0
80	685.0
81	685.0
82	482.5
83	280.0
84	280.0
85	280.0
86	201.5
87	123.0
88	123.0
89	123.0
90	90.5
91	58.0
92	58.0
93	58.0
94	48.5
95	39.0
96	39.0
97	39.0
98	229.5
99	420.0
100	420.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008331104062368633
2	0.003821607368059006
3	0.004891657431115527
4	0.013146329346122981
5	0.03462376275461459
6	0.04532426338517981
7	0.0661138074674208
8	0.08430465853938165
9	0.09485229487522452
10	0.11441892459968662
11	0.11571827110482669
12	0.1275652539458096
13	0.11824053196774563
14	0.11931058203080215
15	0.11036802078954408
16	0.10861008140023694
17	0.12030419994649749
18	0.1261130431459472
19	0.12618947529330837
20	0.11678832116788322
21	0.1241258073145565
22	0.1444567585126304
23	0.13520846868192762
24	0.12909389689303322
25	0.12703022891428134
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1308350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.148865633737046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79364704689321	31.23830766664661
2	11.922287777101296	9.334880844649685
3	3.2404417496971822	3.8057885595854026
4	1.34905962111807	2.1125661535620615
5	0.7260471896864417	1.421196193639345
6	0.4854024518008947	1.140177321830385
7	0.3413381077869068	0.9354099802196569
8	0.25038670464849394	0.7841884365406474
9	0.1931518109097782	0.6805506862997902
>10	1.3549049661156731	10.408691569762649
>50	0.16833728623406624	4.589270483053832
>100	0.14105561913989048	11.299552755560672
>500	0.01746029207663952	4.717958601582278
>1k	0.014517546221026119	10.779363000082578
>5k	0.001177098342245361	2.7728346447593966
>10k+	7.84732228163574E-4	3.9792631022249974
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	14249	1.0890816677494555	No Hit
GTCCTACAGTGGACATTTCTAAATT	13622	1.0411587113539955	No Hit
CTGTAGGACGTGGAATATGGCAAGA	12697	0.970458975044904	No Hit
CTTTAGGACGTGAAATATGGCGAGG	11243	0.859326632781748	No Hit
GTCCTACAGTGTGCATTTCTCATTT	8021	0.6130622539840257	No Hit
CTGAAGGACCTGGAATATGGCGAGA	6053	0.4626437879772232	No Hit
CTGTAGGACCTGGAATATGGCGAGA	5756	0.43994344021095266	No Hit
GTACATGGAAGCAGTGGTATCAACG	5643	0.43130660755913935	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	5609	0.42870791454885926	No Hit
ATTTAGAAATGTCCACTGTAGGACG	5021	0.38376581190048537	No Hit
TCCATGTACTCTGCGTTGATACCAC	4518	0.34532044177781174	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4376	0.3344670768525242	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3967	0.30320632858180147	No Hit
GAATATGGCAAGAAAACTGAAAATC	3725	0.2847097489203959	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	3199	0.24450643940841518	No Hit
GGAATATGGCGAGAAAACTGAAAAT	3185	0.24343638934535863	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3053	0.23334734589368286	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2905	0.22203538808422824	No Hit
CTGTAGGACATGGAATATGGCAAGA	2895	0.22127106661061643	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	2792	0.21339855543241487	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2690	0.20560247640157447	No Hit
ACCTGGAATATGGCGAGAAAACTGA	2662	0.20346237627546143	No Hit
GCCATATTCCACGTCCTACAGTGGA	2618	0.2000993617915695	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2559	0.1955898650972599	No Hit
GACCTGGAATATGGCGAGAAAACTG	2553	0.19513127221309284	No Hit
GATATACACTGTTCTACAAATCCCG	2540	0.1941376542973975	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2507	0.19161539343447853	No Hit
CCATATTTCACGTCCTAAAGTGTGT	2429	0.1856536859403065	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2417	0.18473650017197232	No Hit
GTCCACTGTAGGACGTGGAATATGG	2353	0.1798448427408568	No Hit
ATACACACTTTAGGACGTGAAATAT	2332	0.17823976764627203	No Hit
TTGTAGAACAGTGTATATCAATGAG	2281	0.17434172813085183	No Hit
CATGTACTCTGCGTTGATACCACTG	2203	0.16838002063667978	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	2145	0.16394695608973134	No Hit
CCACTGTAGGACGTGGAATATGGCA	2116	0.16173042381625713	No Hit
CACTTTAGGACGTGAAATATGGCGA	2068	0.15806168074292046	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2024	0.15469866625902856	No Hit
GTGTATTTCTCATTTTCCGTGATTT	2012	0.15378148049069437	No Hit
GAAATACACACTTTAGGACGTGAAA	1941	0.1483547980280506	No Hit
CTACAGTGGACATTTCTAAATTTTC	1937	0.14804906943860588	No Hit
ATTCCACGTCCTACAGTGGACATTT	1924	0.14705545152291052	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	1912	0.14613826575457636	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1883	0.14392173348110215	No Hit
GTGTATATCAATGAGTTACAATGAG	1820	0.1391065081973478	No Hit
GTAGGACGTGGAATATGGCAAGAAA	1703	0.13016394695608974	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1609	0.12297932510413881	No Hit
GTGTATATCAATGAGTTACAATGAA	1590	0.12152711430427637	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	1587	0.12129781786219285	No Hit
CCCATGTACTCTGCGTTGATACCAC	1534	0.1172469140520503	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	1513	0.1156418389574655	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	1500	0.11464822104177017	No Hit
GTCCTACAGTGGACATTTCTAAATA	1496	0.11434249245232544	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1487	0.11365460312607484	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1465	0.11197309588412888	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	1449	0.11075018152634998	No Hit
ACGTGGAATATGGCAAGAAAACTGA	1444	0.11036802078954408	No Hit
ACGTCCTACAGTGGACATTTCTAAA	1428	0.10914510643176518	No Hit
GTATCAACGCAGAGTACATGGGAAA	1393	0.1064699812741239	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1376	0.10517063476898385	No Hit
GCGTTGATACCACTGCTTCCATGTA	1350	0.10318339893759315	No Hit
CCTTCAGTGTGCATTTCTCATTTTT	1322	0.10104329881148011	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1313	0.10035540948522949	No Hit
CACTGAAGGACCTGGAATATGGCGA	1310	0.10012611304314595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.643214736118012E-5	0.0
8	0.0	0.0	0.0	7.643214736118012E-5	0.0
9	0.0	0.0	0.0	7.643214736118012E-5	0.0
10	0.0	0.0	0.0	7.643214736118012E-5	0.0
11	0.0	0.0	0.0	1.5286429472236024E-4	0.0
12	0.0	0.0	0.0	1.5286429472236024E-4	0.0
13	0.0	0.0	0.0	1.5286429472236024E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGCG	25	0.0060467487	18.991735	3
CGGACCA	45	6.758109E-4	14.778701	17
GTACTAG	65	3.391764E-6	14.606792	1
GTGTCCG	40	0.0052709645	14.253072	9
TCCAACG	320	0.0	13.953996	18
TAGAAAT	1310	0.0	13.920804	4
TAGGACC	1875	0.0	13.879816	4
CTAGGAC	295	0.0	13.8414345	3
CCAACGT	125	7.2759576E-12	13.680854	19
GTCCTAG	320	0.0	13.648222	1
ATAATAC	85	2.7122223E-7	13.405932	3
CCAACGA	200	0.0	13.300831	19
GGCGAAG	80	1.997305E-6	13.063315	19
GTTCTAG	110	2.7685019E-9	12.946931	1
CCACCTT	1220	0.0	12.92753	13
AAATGTC	1400	0.0	12.759404	7
GGCGAGC	60	4.0890966E-4	12.667458	19
CTTATAC	60	4.106292E-4	12.661158	3
CACCTTT	1280	0.0	12.618941	14
CTAGAGT	320	0.0	12.466188	4
>>END_MODULE