FastQCFastQC Report
Thu 2 Feb 2017
SRR4062441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308350
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA73710.5633813581992586No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA55920.42740856804371924No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC52630.4022623915618909No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA51440.3931669660259105No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA33260.25421332212328507No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26090.1994114724653189No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA25400.1941376542973975No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA22970.1755646424886307No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21220.1621890167004242No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA20680.15806168074292046No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT18680.14277525127068444No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA16790.12832957541942142No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG16130.12328505369358352No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT15900.12152711430427637No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15040.11495394963121489No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG14530.11105591011579469No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA14090.10769289563190279No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA13920.1063935491267627No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT13670.1044827454427332No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC13480.1030305346428708No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT13130.10035540948522949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT401.8202303E-532.99988643
TACGACG300.00574528229.333234
CGTTTAC300.00574528229.3332324
TAGGACC23550.025.9701864
CAGCGTA551.5937537E-423.99991838
TCGTCTA651.8135883E-523.69222527
TGTAGGA82450.022.0933132
GACGTGT904.082467E-721.9999247
GGACGTA1004.6249625E-821.9999246
CGTGTAA1004.6249625E-821.9999249
CTGTAGG80400.021.9759181
AGGACCT51250.021.5921215
ACGTGTA1057.7476216E-820.9523098
GGACCTG50800.020.8739436
GACGTAA856.114251E-620.7058097
TATCTCG856.114251E-620.70580941
ACTGTTC10850.020.6819558
TCGTATA755.473224E-520.53326244
CAGTCGT755.473224E-520.53326241
CTAGGTC1502.1827873E-1120.5332624
Produced by FastQC (version 0.11.5)