##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1308350
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313529254404404	32.0	32.0	32.0	32.0	32.0
2	31.556846409599878	32.0	32.0	32.0	32.0	32.0
3	31.60810562922765	32.0	32.0	32.0	32.0	32.0
4	31.67934344785417	32.0	32.0	32.0	32.0	32.0
5	31.66684755608209	32.0	32.0	32.0	32.0	32.0
6	35.285325027706655	36.0	36.0	36.0	36.0	36.0
7	35.30690488019261	36.0	36.0	36.0	36.0	36.0
8	35.25996178392632	36.0	36.0	36.0	36.0	36.0
9	35.357673405434326	36.0	36.0	36.0	36.0	36.0
10	35.28151106355333	36.0	36.0	36.0	36.0	36.0
11	35.3545373944281	36.0	36.0	36.0	36.0	36.0
12	35.309786372148125	36.0	36.0	36.0	36.0	36.0
13	35.316852524171665	36.0	36.0	36.0	36.0	36.0
14	35.2938999503191	36.0	36.0	36.0	36.0	36.0
15	35.26992395001338	36.0	36.0	36.0	36.0	36.0
16	35.299702678946765	36.0	36.0	36.0	36.0	36.0
17	35.311898192379715	36.0	36.0	36.0	36.0	36.0
18	35.311735391905835	36.0	36.0	36.0	36.0	36.0
19	35.253634730767764	36.0	36.0	36.0	36.0	36.0
20	35.22147590476555	36.0	36.0	36.0	36.0	36.0
21	35.188982305957886	36.0	36.0	36.0	36.0	36.0
22	35.1731585584897	36.0	36.0	36.0	36.0	36.0
23	35.1571788894409	36.0	36.0	36.0	36.0	36.0
24	35.15101081514885	36.0	36.0	36.0	36.0	36.0
25	35.111872969771085	36.0	36.0	36.0	36.0	36.0
26	35.08938281041006	36.0	36.0	36.0	36.0	36.0
27	35.02974815607445	36.0	36.0	36.0	36.0	36.0
28	34.97765200443306	36.0	36.0	36.0	36.0	36.0
29	34.938636450491074	36.0	36.0	36.0	36.0	36.0
30	34.888000152864294	36.0	36.0	36.0	36.0	36.0
31	34.87324110520885	36.0	36.0	36.0	36.0	36.0
32	34.8512584553063	36.0	36.0	36.0	36.0	36.0
33	34.864011923414985	36.0	36.0	36.0	36.0	36.0
34	34.815772537929455	36.0	36.0	36.0	36.0	36.0
35	34.80636297626782	36.0	36.0	36.0	36.0	36.0
36	34.73078992624298	36.0	36.0	36.0	36.0	36.0
37	34.63803263652692	36.0	36.0	36.0	32.0	36.0
38	34.59152367485765	36.0	36.0	36.0	32.0	36.0
39	34.37553406962969	36.0	36.0	36.0	32.0	36.0
40	34.44036534566439	36.0	36.0	36.0	32.0	36.0
41	34.41548591737685	36.0	36.0	36.0	32.0	36.0
42	34.383383651163676	36.0	36.0	36.0	32.0	36.0
43	34.37352619711851	36.0	36.0	36.0	32.0	36.0
44	34.28082164558413	36.0	36.0	36.0	32.0	36.0
45	34.22441854243895	36.0	36.0	36.0	32.0	36.0
46	34.10252302518439	36.0	36.0	36.0	32.0	36.0
47	34.09371880612986	36.0	36.0	36.0	32.0	36.0
48	34.05091298200023	36.0	36.0	36.0	32.0	36.0
49	34.00540910306875	36.0	36.0	36.0	32.0	36.0
50	33.585422860855275	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	7.0
21	32.0
22	86.0
23	293.0
24	793.0
25	1883.0
26	4099.0
27	8486.0
28	14484.0
29	22118.0
30	30620.0
31	42631.0
32	61834.0
33	93110.0
34	191834.0
35	836039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.460413148655086	21.57720565470753	13.108598485340735	25.853782711296645
2	10.30202414419868	21.416625774092605	49.19827624424256	19.08307383746616
3	18.67673945078664	27.459827079116806	30.774169484478087	23.089263985618462
4	10.317852185663709	18.31376151837743	40.583669126774	30.784717169184862
5	9.189360645087325	42.05090381014255	37.1153743264417	11.644361218328429
6	28.774258737960917	39.56997515177595	19.586071848131567	12.069694262131566
7	25.81268453600683	32.72895840482929	23.4905212600002	17.96783579916368
8	21.59307524744908	45.45763748232506	20.226086291894372	12.723200978331487
9	26.666029732105322	15.19287652386594	22.07956586540299	36.061527878625746
10	15.493331295142736	30.102801238200787	36.14690258722818	18.256964879428285
11	31.91294378415562	20.242060610692857	30.887071502273855	16.95792410287767
12	26.736576604119694	23.92693086712271	34.77907287805251	14.557419650705086
13	31.067222073604157	24.314671150685978	26.6398899377078	17.978216838002066
14	17.853785302098064	27.528260786486797	27.709328543585432	26.908625367829707
15	18.684679176061454	42.56949592998816	22.73390147896205	16.011923414988345
16	16.46172660220889	28.40883555623495	37.524668475560816	17.604769365995338
17	16.908013910650823	33.95895593686705	32.452249015936104	16.68078113654603
18	17.478732754996752	26.689417969197844	41.266174953185306	14.565674322620096
19	23.996484121221386	26.184277907287807	28.565215729735925	21.254022241754882
20	23.828562693468875	29.691443421102914	31.76886918637979	14.711124699048419
21	19.445179042305195	27.569610578209197	27.323575495853557	25.661634883632058
22	22.296786028203464	35.2281881759468	27.461994114724654	15.01303168112508
23	16.436274697137616	34.56108839377842	31.843466962204303	17.159169946879658
24	23.332059464210648	28.426873543012192	32.71655138151106	15.524515611266098
25	19.51098712118317	31.699621660870562	32.353192952956014	16.436198264990253
26	14.309015171781251	33.77230863301104	34.336760041273365	17.581916153934348
27	17.020216302977033	33.62930408529828	31.72079336568961	17.62968624603508
28	15.607673787595063	33.60369931593228	29.194252302518443	21.594374593954218
29	19.341002025451907	29.89559368670463	28.47655443879696	22.286849849046508
30	16.527381816792143	29.973783773455114	32.25543623648107	21.24339817327168
31	21.846294951656667	27.57381434631406	30.784728856957237	19.795161845072037
32	17.443879695800053	32.92933847976459	31.291168265372416	18.335613559062942
33	15.485229487522453	33.698093017923334	34.9974395230634	15.81923797149081
34	17.550884702105705	32.53945809607521	34.44880956930485	15.460847632514236
35	16.59769939236443	33.99778346772653	32.70523942370161	16.699277716207437
36	15.339626246799403	31.795544005808846	35.259983949249055	17.604845798142698
37	16.684908472503533	31.62510031719341	28.852371307371882	22.837619902931173
38	18.252837543470786	35.51587877861429	28.52776397752895	17.703519700385982
39	21.552642641495012	28.77471624565292	30.40929414911912	19.263346963732946
40	23.4920319486376	28.572323919440517	32.067030993235754	15.86861313868613
41	22.299461153361104	26.41930676042343	34.06267436083617	17.218557725379295
42	19.06653418427791	33.347345893682885	32.48419765353307	15.101922268506135
43	15.46627431497688	33.93831925707953	33.41330683685558	17.18209959108801
44	15.006764245041465	32.963732946077116	32.47120418848167	19.55829862039974
45	15.917300416555202	33.71276799021668	31.21664692169526	19.153284671532848
46	17.432491305843236	28.16639278480529	32.64187717353919	21.759238735812282
47	22.68345626170367	28.256353422249397	30.870867887033288	18.189322429013643
48	15.706041961248902	31.717965376237245	30.298314671150685	22.277677991363166
49	20.17036725646807	28.418695303244547	31.14824014980701	20.262697290480375
50	15.90224328352505	35.87449841403294	28.419765353307604	19.803492949134405
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	13.5
2	16.0
3	57.5
4	99.0
5	132.0
6	165.0
7	202.0
8	239.0
9	325.0
10	411.0
11	613.5
12	816.0
13	1499.0
14	2182.0
15	3121.0
16	4060.0
17	5105.5
18	6151.0
19	6833.0
20	7515.0
21	8540.0
22	9565.0
23	10695.5
24	11826.0
25	14382.5
26	16939.0
27	25628.5
28	34318.0
29	47143.0
30	59968.0
31	77067.5
32	94167.0
33	121469.0
34	148771.0
35	175012.0
36	201253.0
37	195028.0
38	188803.0
39	163453.0
40	138103.0
41	115912.5
42	93722.0
43	79058.5
44	64395.0
45	59486.5
46	54578.0
47	52283.5
48	49989.0
49	43646.5
50	37304.0
51	30996.5
52	24689.0
53	20490.0
54	16291.0
55	14011.5
56	11732.0
57	10653.0
58	9574.0
59	7828.5
60	6083.0
61	5230.0
62	4377.0
63	3683.0
64	2989.0
65	2681.0
66	2373.0
67	1956.5
68	1540.0
69	1368.0
70	1196.0
71	993.0
72	790.0
73	652.5
74	515.0
75	436.5
76	358.0
77	269.0
78	180.0
79	157.0
80	134.0
81	101.0
82	68.0
83	59.0
84	50.0
85	36.0
86	22.0
87	14.0
88	6.0
89	8.0
90	10.0
91	6.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015362861619597202
2	0.003363014483891925
3	4.5859288416708066E-4
4	4.5859288416708066E-4
5	0.0
6	5.350250315282608E-4
7	7.64321473611801E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1308350.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.65746892784653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66118281801931	45.45054745305105
2	11.343431508133268	12.626933733981652
3	3.0120345921022653	5.029266651585922
4	1.2274224076111109	2.732608980518319
5	0.6565630456703285	1.8271318656784294
6	0.40560494366337196	1.354496672935504
7	0.2954595153148183	1.1511170155149766
8	0.19413056803742657	0.8643852846790612
9	0.14702765896191422	0.736486862418608
>10	0.8856277999887313	9.414735055536164
>50	0.09057820267216388	3.5048685085105924
>100	0.07042206583505907	7.807914172213197
>500	0.006640973046482653	2.6288434747679768
>1k	0.0033204865232413267	3.0710781059989003
>5k	5.534144205402211E-4	1.7995861626095555
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	7371	0.5633813581992586	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	5592	0.42740856804371924	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	5263	0.4022623915618909	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	5144	0.3931669660259105	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3326	0.25421332212328507	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2609	0.1994114724653189	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2540	0.1941376542973975	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	2297	0.1755646424886307	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2122	0.1621890167004242	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	2068	0.15806168074292046	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	1868	0.14277525127068444	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1679	0.12832957541942142	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	1613	0.12328505369358352	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	1590	0.12152711430427637	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1504	0.11495394963121489	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1453	0.11105591011579469	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA	1409	0.10769289563190279	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	1392	0.1063935491267627	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	1367	0.1044827454427332	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC	1348	0.1030305346428708	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT	1313	0.10035540948522949	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.643214736118012E-5	0.0
4	0.0	0.0	0.0	7.643214736118012E-5	0.0
5	0.0	0.0	0.0	7.643214736118012E-5	0.0
6	0.0	0.0	0.0	7.643214736118012E-5	0.0
7	0.0	0.0	0.0	7.643214736118012E-5	0.0
8	0.0	0.0	0.0	7.643214736118012E-5	0.0
9	0.0	0.0	0.0	7.643214736118012E-5	0.0
10	0.0	0.0	0.0	1.5286429472236024E-4	0.0
11	0.0	0.0	0.0	2.2929644208354033E-4	0.0
12	0.0	0.0	0.0	2.2929644208354033E-4	0.0
13	0.0	0.0	0.0	2.2929644208354033E-4	0.0
14	0.0	0.0	0.0	2.2929644208354033E-4	0.0
15	0.0	0.0	0.0	3.057285894447205E-4	0.0
16	0.0	0.0	0.0	3.8216073680590057E-4	0.0
17	0.0	0.0	0.0	4.5859288416708066E-4	0.0
18	0.0	0.0	0.0	5.350250315282608E-4	0.0
19	0.0	0.0	0.0	6.11457178889441E-4	0.0
20	0.0	0.0	0.0	8.407536209729812E-4	0.0
21	0.0	0.0	0.0	0.0014522107998624222	0.0
22	0.0	0.0	0.0	0.002675125157641304	0.0
23	0.0	0.0	0.0	0.0038980395154201855	0.0
24	0.0	0.0	0.0	0.006343868230977949	0.0
25	0.0	0.0	0.0	0.007413918294034471	0.0
26	0.0	0.0	0.0	0.009554018420147513	0.0
27	0.0	0.0	0.0	0.012534872167233539	0.0
28	0.0	0.0	0.0	0.01979592616654565	0.0
29	0.0	0.0	0.0	0.031260748270722664	0.0
30	0.0	0.0	0.0	0.051897428058241296	0.0
31	0.0	0.0	0.0	0.11327244238926892	0.0
32	0.0	0.0	0.0	0.19987006534948598	0.0
33	0.0	0.0	0.0	0.285168341804563	0.0
34	0.0	0.0	0.0	0.39026254442618563	0.0
35	0.0	0.0	0.0	0.5111782015515726	0.0
36	0.0	0.0	0.0	0.6693927465892154	0.0
37	0.0	0.0	0.0	0.9476057629839111	0.0
38	0.0	0.0	0.0	1.2573088240914128	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAT	40	1.8202303E-5	32.999886	43
TACGACG	30	0.005745282	29.33323	4
CGTTTAC	30	0.005745282	29.33323	24
TAGGACC	2355	0.0	25.970186	4
CAGCGTA	55	1.5937537E-4	23.999918	38
TCGTCTA	65	1.8135883E-5	23.692225	27
TGTAGGA	8245	0.0	22.093313	2
GACGTGT	90	4.082467E-7	21.999924	7
GGACGTA	100	4.6249625E-8	21.999924	6
CGTGTAA	100	4.6249625E-8	21.999924	9
CTGTAGG	8040	0.0	21.975918	1
AGGACCT	5125	0.0	21.592121	5
ACGTGTA	105	7.7476216E-8	20.952309	8
GGACCTG	5080	0.0	20.873943	6
GACGTAA	85	6.114251E-6	20.705809	7
TATCTCG	85	6.114251E-6	20.705809	41
ACTGTTC	1085	0.0	20.681955	8
TCGTATA	75	5.473224E-5	20.533262	44
CAGTCGT	75	5.473224E-5	20.533262	41
CTAGGTC	150	2.1827873E-11	20.533262	4
>>END_MODULE