FastQCFastQC Report
Thu 2 Feb 2017
SRR4062440_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062440_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375535
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG183264.879971241029465No Hit
TCCATGTACTCTGCGTTGATACCAC152444.059275433714568No Hit
GAGTACATGGAAGCAGTGGTATCAA70611.880250842131892No Hit
CATGTACTCTGCGTTGATACCACTG60331.6065080485174483No Hit
CCCATGTACTCTGCGTTGATACCAC47841.273915880011184No Hit
GTACATGGGAAGCAGTGGTATCAAC45081.2004207330874619No Hit
GCTTCCATGTACTCTGCGTTGATAC43871.1682000346172794No Hit
CATGGAAGCAGTGGTATCAACGCAG40911.0893791524092296No Hit
GCGTTGATACCACTGCTTCCATGTA39021.0390509539723327No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32180.8569108072483258No Hit
GTATCAACGCAGAGTACATGGAAGC31010.8257552558350088No Hit
GTATCAACGCAGAGTACTTTTTTTT28340.7546566897892341No Hit
ACTCTGCGTTGATACCACTGCTTCC25080.6678472046546926No Hit
CAGTGGTATCAACGCAGAGTACATG23790.633496212070779No Hit
ACGCAGAGTACATGGAAGCAGTGGT23480.625241322380071No Hit
TATCAACGCAGAGTACTTTTTTTTT20780.5533438960416472No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20370.5424261387087754No Hit
TATCAACGCAGAGTACATGGAAGCA19160.5102054402385929No Hit
GTACATGGGGTGGTATCAACGCAAA18170.4838430505811709No Hit
GTATCAACGCAGAGTACATGGGAAG17640.46972985207770246No Hit
GAGTACATGGGAAGCAGTGGTATCA14980.3988975727961442No Hit
ATACCACTGCTTCCATGTACTCTGC13820.36800830814704355No Hit
GGTATCAACGCAGAGTACATGGAAG13740.3658780140333125No Hit
GTCCTAAAGTGTGTATTTCTCATTT13730.3656117272690961No Hit
GTCCTACAGTGGACATTTCTAAATT13620.3626825728627159No Hit
GTCCTACAGTGTGCATTTCTCATTT12910.34377621260335256No Hit
AAGCAGTGGTATCAACGCAGAGTAC12640.3365864699695102No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12500.3328584552704808No Hit
GTACTCTGCGTTGATACCACTGCTT12430.3309944479209661No Hit
GCTTCCCATGTACTCTGCGTTGATA12010.31981040382387793No Hit
CTTTAGGACGTGAAATATGGCGAGG11680.31102294060473723No Hit
GCGTTGATACCACTGCTTCCCATGT11680.31102294060473723No Hit
GTGGTATCAACGCAGAGTACATGGA10950.2915840068169412No Hit
AAAAAGTACTCTGCGTTGATACCAC10940.29131772005272477No Hit
CTGTAGGACGTGGAATATGGCAAGA10860.2891874259389937No Hit
TATCAACGCAGAGTACATGGGAAGC10770.28679084506104624No Hit
GTACATGGGTGGTATCAACGCAAAA10610.28253025683358407No Hit
GAAGCAGTGGTATCAACGCAGAGTA10510.27986738919142023No Hit
GGTATCAACGCAGAGTACTTTTTTT10380.27640566125660726No Hit
CCACTGCTTCCATGTACTCTGCGTT10300.27427536714287615No Hit
CTGTAGGACCTGGAATATGGCGAGA10190.2713462127364959No Hit
ACATGGAAGCAGTGGTATCAACGCA9820.2614936024604897No Hit
GATATACACTGTTCTACAAATCCCG9660.2572330142330275No Hit
TTGTAGAACAGTGTATATCAATGAG9520.2535049995339982No Hit
ACGCAGAGTACATGGGAAGCAGTGG8980.2391255142663134No Hit
GTACATGGGAGTGGTATCAACGCAA8960.23859294073788062No Hit
TGGAAGCAGTGGTATCAACGCAGAG8910.2372615069167987No Hit
GGTATCAACGCAGAGTACATGGGAA8480.22581117605549417No Hit
GTTGATACCACTGCTTCCATGTACT8220.2188877201858682No Hit
GTGTATATCAATGAGTTACAATGAA7990.21276312460889132No Hit
CTGCTTCCATGTACTCTGCGTTGAT7840.20876882314564554No Hit
GTGTATATCAATGAGTTACAATGAG7640.20344308786131785No Hit
GCAGAGTACATGGAAGCAGTGGTAT7250.19305790405687887No Hit
CCCCATGTACTCTGCGTTGATACCA7210.1919927570000133No Hit
GATACCACTGCTTCCATGTACTCTG7140.1901287496504986No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6850.18240643348822347No Hit
CCATGTACTCTGCGTTGATACCACT6800.18107499966714155No Hit
GTTCTACAGTGTGGTTTTTATCATT6790.18080871290292516No Hit
GCAGTGGTATCAACGCAGAGTACAT6520.17361897026908277No Hit
CTGAAGGACCTGGAATATGGCGAGA6140.16350007322886018No Hit
CTTCCATGTACTCTGCGTTGATACC5860.1560440438308014No Hit
GTATCAACGCAGAGTACATGGGGTG5730.1525823158959884No Hit
GTCCTTCAGTGTGCATTTCTCATTT5500.14645772031901155No Hit
GATATACACTGTTCTACAATGCCGG5410.14406113944106408No Hit
ATTTAGAAATGTCCACTGTAGGACG5030.13394224240084146No Hit
AAAGTACTCTGCGTTGATACCACTG4900.13048051446602846No Hit
CTGTAGGACATGGAATATGGCAAGA4750.12648621300278268No Hit
ATCAACGCAGAGTACATGGAAGCAG4710.12542106594591715No Hit
CTGTAGAACATATTAGATGAGTGAG4650.12382334536061884No Hit
TATCAACGCAGAGTACATGGGGTGG4630.12329077183218609No Hit
TCCATGTACTCTGCGTTGATACCAA4300.11450330861304539No Hit
TTTCTAAATTTTCCACCTTTTTCAG4250.11317187479196347No Hit
GAATATGGCAAGAAAACTGAAAATC4210.11210672773509793No Hit
GTTCTACAAATCCCGTTTCCAACGA4150.11050900714979961No Hit
CTGCTTCCCATGTACTCTGCGTTGA4150.11050900714979961No Hit
ATGTTCTACAGTGTGGTTTTTATCA4110.1094438600929341No Hit
GTATCAACGCAGAGTACATGGGAGT4050.10784613950763576No Hit
GTGGTATCAACGCAGAGTACATGGG3980.10598213215812108No Hit
TGATACCACTGCTTCCATGTACTCT3860.10278669098752446No Hit
CACTGCTTCCATGTACTCTGCGTTG3810.10145525716644253No Hit
GTACATGGAAGCAGTGGTATCAACC3800.10118897040222614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATC250.006018119419.0030697
ATCGTAA307.713268E-418.99814
AATCGTA307.713268E-418.99813
GACCAGG350.00216373816.2883436
CGTAAAT350.002165666816.28617116
TTTTGGT350.002165666816.28617116
TCGTAAA350.00216759716.28415
CTAGGAC951.8189894E-1115.9983163
CCAACGT551.1234621E-515.54589219
GGACTTG508.673922E-515.2024556
CAACTTT508.693682E-515.198400514
ATTTAGA1800.014.7742541
TTAGAAA1900.014.4984743
AGACCTG400.00526358514.25236
AAATCGT400.00526824514.25040112
TTTGGTA400.00526824514.25040117
TAAGACC400.005272908614.24854
GTTCTAA400.005277575514.2466011
TAGAAAT1950.014.12671854
CCAACTT551.9556646E-413.81672713
Produced by FastQC (version 0.11.5)