##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062440_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375535
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74117459091696	32.0	32.0	32.0	32.0	32.0
2	30.790695940458278	32.0	32.0	32.0	32.0	32.0
3	30.807858122412025	32.0	32.0	32.0	32.0	32.0
4	30.737790086143768	32.0	32.0	32.0	32.0	32.0
5	30.812262505492164	32.0	32.0	32.0	32.0	32.0
6	34.312844342071976	36.0	36.0	36.0	32.0	36.0
7	34.036910008388034	36.0	36.0	36.0	32.0	36.0
8	34.03259616280773	36.0	36.0	36.0	32.0	36.0
9	33.995369273170276	36.0	36.0	36.0	32.0	36.0
10	33.996445071697714	36.0	36.0	36.0	32.0	36.0
11	34.230891927516744	36.0	36.0	36.0	32.0	36.0
12	34.09807341526089	36.0	36.0	36.0	32.0	36.0
13	34.07640033552132	36.0	36.0	36.0	32.0	36.0
14	34.00943986579147	36.0	36.0	36.0	32.0	36.0
15	34.086154419694566	36.0	36.0	36.0	32.0	36.0
16	33.993939313246436	36.0	36.0	36.0	32.0	36.0
17	33.951277510751325	36.0	36.0	36.0	32.0	36.0
18	33.98368993569175	36.0	36.0	36.0	32.0	36.0
19	33.90753990972879	36.0	36.0	36.0	32.0	36.0
20	33.827930818698654	36.0	36.0	36.0	32.0	36.0
21	33.59422956581943	36.0	36.0	36.0	27.0	36.0
22	33.53785932070247	36.0	36.0	36.0	27.0	36.0
23	33.388688138256086	36.0	36.0	36.0	27.0	36.0
24	33.39001690920953	36.0	36.0	36.0	21.0	36.0
25	32.704866390616054	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	12.0
5	50.0
6	74.0
7	17.0
8	52.0
9	61.0
10	39.0
11	11.0
12	28.0
13	25.0
14	162.0
15	211.0
16	275.0
17	398.0
18	496.0
19	688.0
20	1007.0
21	1351.0
22	1881.0
23	2551.0
24	3040.0
25	3929.0
26	5044.0
27	6043.0
28	7891.0
29	10260.0
30	13320.0
31	18353.0
32	26298.0
33	37019.0
34	78060.0
35	156887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.234716840437294	16.681180841289496	14.226554281681514	21.857548036591698
2	8.58835747763102	23.779825308904982	43.40035151256924	24.23146570089476
3	20.930232558139537	31.709447458051393	27.332571720738496	20.027748263070578
4	11.121468196792913	19.688879064453328	34.92027559317249	34.269377145581274
5	9.321363400242417	42.1354076481479	35.54830374149206	12.994925210117614
6	29.253366299175674	33.198610319232266	22.581246769578353	14.966776612013705
7	24.06607491652699	33.007080216696735	24.38930799342342	18.53753687335286
8	27.43906014296147	36.37228615747083	23.255225129662104	12.933428569905598
9	27.055828768675116	18.171737768715097	18.51318753081977	36.25924593179001
10	15.085979364952411	31.560425497880505	34.89669146071609	18.456903676450985
11	32.88189093933787	22.798018629996747	22.170709208891637	22.14938122177374
12	21.75853711214034	25.669863667511912	33.90740696300918	18.66419225733857
13	34.72328127249556	23.311133269701443	24.43651119512416	17.529074262678833
14	24.335745250371918	22.306322350845946	25.56720930356562	27.790723095216514
15	25.23540138201672	29.40624466814537	31.008200392424502	14.350153557413412
16	22.08890369876708	25.87804065311563	34.83852049316894	17.19453515494835
17	20.6087250952466	27.0268396791076	34.36742659546389	17.997008630181906
18	23.242085954018147	22.70270702632372	39.10963816552508	14.945568854133063
19	20.58127133105802	34.79095563139932	26.532369880546074	18.095403156996586
20	19.865150206353775	24.975472160902623	39.29679751735611	15.862580115387487
21	19.90070842565263	30.434307318620903	23.570550609640666	26.094433646085797
22	18.252391454500362	34.09106061373776	26.32560049709185	21.330947434670023
23	17.27811376702612	32.59469030324363	26.043699934935415	24.083495994794834
24	18.087764347633637	35.25906134758298	27.570014958922958	19.08315934586043
25	24.05245209267105	28.157537588622226	25.61172333067589	22.178286988030834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	222.0
1	222.0
2	546.0
3	870.0
4	870.0
5	870.0
6	2455.0
7	4040.0
8	4040.0
9	4040.0
10	3596.5
11	3153.0
12	3153.0
13	3153.0
14	2306.0
15	1459.0
16	1459.0
17	1459.0
18	2491.5
19	3524.0
20	3524.0
21	3524.0
22	6097.0
23	8670.0
24	8670.0
25	8670.0
26	14306.5
27	19943.0
28	19943.0
29	19943.0
30	26850.0
31	33757.0
32	33757.0
33	33757.0
34	32942.5
35	32128.0
36	32128.0
37	32128.0
38	31750.5
39	31373.0
40	31373.0
41	31373.0
42	38910.0
43	46447.0
44	46447.0
45	46447.0
46	80859.5
47	115272.0
48	115272.0
49	115272.0
50	81548.5
51	47825.0
52	47825.0
53	47825.0
54	30090.5
55	12356.0
56	12356.0
57	12356.0
58	9041.0
59	5726.0
60	5726.0
61	5726.0
62	4698.0
63	3670.0
64	3670.0
65	3670.0
66	3013.5
67	2357.0
68	2357.0
69	2357.0
70	1833.0
71	1309.0
72	1309.0
73	1309.0
74	1029.0
75	749.0
76	749.0
77	749.0
78	528.0
79	307.0
80	307.0
81	307.0
82	216.0
83	125.0
84	125.0
85	125.0
86	85.5
87	46.0
88	46.0
89	46.0
90	33.5
91	21.0
92	21.0
93	21.0
94	19.0
95	17.0
96	17.0
97	17.0
98	93.0
99	169.0
100	169.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010651470568655385
2	0.0039943014632457694
3	0.004260588227462154
4	0.014379485267684771
5	0.039943014632457696
6	0.05245849255062777
7	0.07029970575312554
8	0.08760834542719054
9	0.09852610276006231
10	0.11849761007629116
11	0.11743246301942561
12	0.13207823505132676
13	0.1227581983037533
14	0.12062790419002224
15	0.11476959537726178
16	0.10917757332871769
17	0.1214267644826714
18	0.12621992623856632
19	0.1318119482871104
20	0.12009533066158946
21	0.12728507329543184
22	0.14752286737587708
23	0.13926797768516916
24	0.13527367622192338
25	0.1299479409375957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	375535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.69514191262558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00799390695806	20.023490419276
2	13.11599893456247	7.002669057679823
3	4.267007728202108	3.4172513053987608
4	2.052999881265059	2.192204927078969
5	1.1549999065112049	1.5416443206692947
6	0.7469999393224448	1.1964761633361216
7	0.49299995995269774	0.9212492725699196
8	0.3639999704315906	0.7773624693490262
9	0.3069999750618084	0.737586711130274
>10	1.9759998394857765	10.420981701915492
>50	0.24299998026064965	4.497063241295908
>100	0.20599998326622976	10.623330861175988
>500	0.023999998050434533	4.758408659390928
>1k	0.03699999699441991	19.433260879533318
>5k	0.0019999998375362107	3.495461598096203
>10k+	0.0019999998375362107	8.961558412103981
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	18326	4.879971241029465	No Hit
TCCATGTACTCTGCGTTGATACCAC	15244	4.059275433714568	No Hit
GAGTACATGGAAGCAGTGGTATCAA	7061	1.880250842131892	No Hit
CATGTACTCTGCGTTGATACCACTG	6033	1.6065080485174483	No Hit
CCCATGTACTCTGCGTTGATACCAC	4784	1.273915880011184	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4508	1.2004207330874619	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4387	1.1682000346172794	No Hit
CATGGAAGCAGTGGTATCAACGCAG	4091	1.0893791524092296	No Hit
GCGTTGATACCACTGCTTCCATGTA	3902	1.0390509539723327	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3218	0.8569108072483258	No Hit
GTATCAACGCAGAGTACATGGAAGC	3101	0.8257552558350088	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2834	0.7546566897892341	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2508	0.6678472046546926	No Hit
CAGTGGTATCAACGCAGAGTACATG	2379	0.633496212070779	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2348	0.625241322380071	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2078	0.5533438960416472	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2037	0.5424261387087754	No Hit
TATCAACGCAGAGTACATGGAAGCA	1916	0.5102054402385929	No Hit
GTACATGGGGTGGTATCAACGCAAA	1817	0.4838430505811709	No Hit
GTATCAACGCAGAGTACATGGGAAG	1764	0.46972985207770246	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1498	0.3988975727961442	No Hit
ATACCACTGCTTCCATGTACTCTGC	1382	0.36800830814704355	No Hit
GGTATCAACGCAGAGTACATGGAAG	1374	0.3658780140333125	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1373	0.3656117272690961	No Hit
GTCCTACAGTGGACATTTCTAAATT	1362	0.3626825728627159	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1291	0.34377621260335256	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1264	0.3365864699695102	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1250	0.3328584552704808	No Hit
GTACTCTGCGTTGATACCACTGCTT	1243	0.3309944479209661	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1201	0.31981040382387793	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1168	0.31102294060473723	No Hit
GCGTTGATACCACTGCTTCCCATGT	1168	0.31102294060473723	No Hit
GTGGTATCAACGCAGAGTACATGGA	1095	0.2915840068169412	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1094	0.29131772005272477	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1086	0.2891874259389937	No Hit
TATCAACGCAGAGTACATGGGAAGC	1077	0.28679084506104624	No Hit
GTACATGGGTGGTATCAACGCAAAA	1061	0.28253025683358407	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1051	0.27986738919142023	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1038	0.27640566125660726	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1030	0.27427536714287615	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1019	0.2713462127364959	No Hit
ACATGGAAGCAGTGGTATCAACGCA	982	0.2614936024604897	No Hit
GATATACACTGTTCTACAAATCCCG	966	0.2572330142330275	No Hit
TTGTAGAACAGTGTATATCAATGAG	952	0.2535049995339982	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	898	0.2391255142663134	No Hit
GTACATGGGAGTGGTATCAACGCAA	896	0.23859294073788062	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	891	0.2372615069167987	No Hit
GGTATCAACGCAGAGTACATGGGAA	848	0.22581117605549417	No Hit
GTTGATACCACTGCTTCCATGTACT	822	0.2188877201858682	No Hit
GTGTATATCAATGAGTTACAATGAA	799	0.21276312460889132	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	784	0.20876882314564554	No Hit
GTGTATATCAATGAGTTACAATGAG	764	0.20344308786131785	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	725	0.19305790405687887	No Hit
CCCCATGTACTCTGCGTTGATACCA	721	0.1919927570000133	No Hit
GATACCACTGCTTCCATGTACTCTG	714	0.1901287496504986	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	685	0.18240643348822347	No Hit
CCATGTACTCTGCGTTGATACCACT	680	0.18107499966714155	No Hit
GTTCTACAGTGTGGTTTTTATCATT	679	0.18080871290292516	No Hit
GCAGTGGTATCAACGCAGAGTACAT	652	0.17361897026908277	No Hit
CTGAAGGACCTGGAATATGGCGAGA	614	0.16350007322886018	No Hit
CTTCCATGTACTCTGCGTTGATACC	586	0.1560440438308014	No Hit
GTATCAACGCAGAGTACATGGGGTG	573	0.1525823158959884	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	550	0.14645772031901155	No Hit
GATATACACTGTTCTACAATGCCGG	541	0.14406113944106408	No Hit
ATTTAGAAATGTCCACTGTAGGACG	503	0.13394224240084146	No Hit
AAAGTACTCTGCGTTGATACCACTG	490	0.13048051446602846	No Hit
CTGTAGGACATGGAATATGGCAAGA	475	0.12648621300278268	No Hit
ATCAACGCAGAGTACATGGAAGCAG	471	0.12542106594591715	No Hit
CTGTAGAACATATTAGATGAGTGAG	465	0.12382334536061884	No Hit
TATCAACGCAGAGTACATGGGGTGG	463	0.12329077183218609	No Hit
TCCATGTACTCTGCGTTGATACCAA	430	0.11450330861304539	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	425	0.11317187479196347	No Hit
GAATATGGCAAGAAAACTGAAAATC	421	0.11210672773509793	No Hit
GTTCTACAAATCCCGTTTCCAACGA	415	0.11050900714979961	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	415	0.11050900714979961	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	411	0.1094438600929341	No Hit
GTATCAACGCAGAGTACATGGGAGT	405	0.10784613950763576	No Hit
GTGGTATCAACGCAGAGTACATGGG	398	0.10598213215812108	No Hit
TGATACCACTGCTTCCATGTACTCT	386	0.10278669098752446	No Hit
CACTGCTTCCATGTACTCTGCGTTG	381	0.10145525716644253	No Hit
GTACATGGAAGCAGTGGTATCAACC	380	0.10118897040222614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	5.325735284327693E-4	0.0
13	0.0	0.0	0.0	5.325735284327693E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTATC	25	0.0060181194	19.003069	7
ATCGTAA	30	7.713268E-4	18.998	14
AATCGTA	30	7.713268E-4	18.998	13
GACCAGG	35	0.002163738	16.288343	6
CGTAAAT	35	0.0021656668	16.286171	16
TTTTGGT	35	0.0021656668	16.286171	16
TCGTAAA	35	0.002167597	16.284	15
CTAGGAC	95	1.8189894E-11	15.998316	3
CCAACGT	55	1.1234621E-5	15.545892	19
GGACTTG	50	8.673922E-5	15.202455	6
CAACTTT	50	8.693682E-5	15.1984005	14
ATTTAGA	180	0.0	14.774254	1
TTAGAAA	190	0.0	14.498474	3
AGACCTG	40	0.005263585	14.2523	6
AAATCGT	40	0.005268245	14.250401	12
TTTGGTA	40	0.005268245	14.250401	17
TAAGACC	40	0.0052729086	14.2485	4
GTTCTAA	40	0.0052775755	14.246601	1
TAGAAAT	195	0.0	14.1267185	4
CCAACTT	55	1.9556646E-4	13.816727	13
>>END_MODULE