FastQCFastQC Report
Thu 2 Feb 2017
SRR4062440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375535
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA75242.003541613964078No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC64521.7180822027241134No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC25870.688883859027787No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA24880.6625214693703649No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG17500.46600183737867307No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16650.4433674624202804No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC16380.436177719786438No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16320.43457999920113966No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA15970.42525996245356623No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG15190.4044895948446882No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12430.3309944479209661No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11620.30942522001943895No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA10030.2670856245090338No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC8940.23806036720944784No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG8830.23513121280306762No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA8400.22368088194176308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.20983397020251107No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7840.20876882314564554No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7740.20610595550348168No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA7720.20557338197504893No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA7550.20104650698337037No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6890.183471580545089No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT6400.17042352909848615No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6260.16669551439945676No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA6180.1645652202857257No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5940.15817433794453245No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5730.1525823158959884No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5610.14938687472539178No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5600.1491205879611754No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG5330.14193084532733302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.14059941150625108No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC5040.13420852916505785No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA4960.13207823505132676No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC4760.1267524997669991No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA4720.12568735271013354No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTATGCGTTGATA4620.1230244850679697No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC4540.12089419095423862No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA4320.11503588214147817No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4320.11503588214147817No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4210.11210672773509793No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG4200.11184044097088154No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA4130.10997643362136685No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT4100.10917757332871769No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA4040.1075798527434194No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC3970.1057158453939047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3840.10225411745909169No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA3760.10012382334536063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC254.43369E-544.0057261
TCATCGT250.002350769735.19989440
CTAGAGT401.8167873E-532.99994
TCGTATA1150.032.5216444
TGCATTA353.2137282E-431.42847812
ATCTGCG353.2137282E-431.42847838
ATACATG1000.030.8040081
TAGGACC3850.030.2856244
CTAGGAC901.6370905E-1129.3332463
GTATGGC300.005740121529.33324215
GGCGACA300.005740121529.33324219
ACGCACT554.949221E-627.99991638
TCCTCGA407.023171E-427.49991644
AAAATCG407.023171E-427.49991632
CTCTAGG756.976734E-826.4034371
CTGTAGG9150.025.4896581
TGGTATA4750.025.47360844
TGTAGGA9350.024.9411012
TGCGGTG1151.2732926E-1124.8694941
GTAGGAT450.00139644624.4443723
Produced by FastQC (version 0.11.5)