FastQCFastQC Report
Thu 2 Feb 2017
SRR4062439_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062439_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067123
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26140.1264559486784289No Hit
GCGCAAGACGGACCAGAGCGAAAGC22020.1065248657191662No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21010.10163884780924985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATTCG350.002168788316.28766614
CGCCAGT4000.013.30384918
CGCATCG4400.012.52422813
GTACCGT853.943005E-612.2946986
CAAGACG4750.012.1979174
ATCGCCA4400.012.09323716
AACGCCG4600.011.9767965
GTATCAA26050.011.91820051
AAGACGG4650.011.8480135
AATCGCT1459.640644E-1111.79451715
GGTATCA10200.011.7284621
AAGACCG658.03362E-411.6908785
CGAACGA1501.7644197E-1011.40219516
TAACGCC4850.011.3588844
CGCAAGA4600.011.3523582
GTATTAT1354.774847E-911.2527041
GTATAAT1354.774847E-911.2527041
ATAACGC5000.011.2053733
TCGCCAG4750.011.202970517
GCCGCAT4850.011.16574511
Produced by FastQC (version 0.11.5)