##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062439_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2067123
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15492159876311	32.0	32.0	32.0	32.0	32.0
2	30.70977827637736	32.0	32.0	32.0	32.0	32.0
3	30.695085391628847	32.0	32.0	32.0	32.0	32.0
4	30.78726519902299	32.0	32.0	32.0	32.0	32.0
5	30.53756597938294	32.0	32.0	32.0	32.0	32.0
6	34.214202541406586	36.0	36.0	36.0	32.0	36.0
7	34.09631260452329	36.0	36.0	36.0	32.0	36.0
8	34.08475112511447	36.0	36.0	36.0	32.0	36.0
9	34.279722106521966	36.0	36.0	36.0	32.0	36.0
10	33.86911422300463	36.0	36.0	36.0	32.0	36.0
11	34.249380902829685	36.0	36.0	36.0	32.0	36.0
12	33.98492494157338	36.0	36.0	36.0	32.0	36.0
13	34.12850759243644	36.0	36.0	36.0	32.0	36.0
14	34.02043129508984	36.0	36.0	36.0	32.0	36.0
15	33.943887712535734	36.0	36.0	36.0	32.0	36.0
16	33.951257375589165	36.0	36.0	36.0	32.0	36.0
17	33.869677324474644	36.0	36.0	36.0	32.0	36.0
18	33.8833688174337	36.0	36.0	36.0	32.0	36.0
19	33.86788401077246	36.0	36.0	36.0	32.0	36.0
20	33.87237237455149	36.0	36.0	36.0	32.0	36.0
21	33.85484221306618	36.0	36.0	36.0	32.0	36.0
22	33.797210906172495	36.0	36.0	36.0	32.0	36.0
23	33.764360901600924	36.0	36.0	36.0	32.0	36.0
24	33.725605588056446	36.0	36.0	36.0	27.0	36.0
25	33.27535178119541	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	16.0
4	96.0
5	222.0
6	374.0
7	97.0
8	308.0
9	389.0
10	227.0
11	77.0
12	176.0
13	130.0
14	294.0
15	367.0
16	634.0
17	849.0
18	1301.0
19	1919.0
20	3021.0
21	4463.0
22	6887.0
23	10447.0
24	14658.0
25	20591.0
26	28252.0
27	36402.0
28	48279.0
29	62820.0
30	79508.0
31	105893.0
32	150187.0
33	210924.0
34	456890.0
35	820425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34234692322467	17.361110775136154	10.967157937311589	26.329384364327584
2	17.22232166614257	19.748046733267216	36.69021068672746	26.33942091386275
3	19.32666024837809	23.354071823551894	28.056371280255053	29.262896647814962
4	12.943818832985448	15.269419357086248	35.35285357208176	36.43390823784654
5	15.038377405242171	36.042287738588406	33.37382399628325	15.545510859886175
6	34.498074870710205	35.021138911811896	16.75890992427744	13.721876293200458
7	30.478830501196484	29.96431609232406	20.687081031178277	18.869772375301178
8	28.10491569585721	32.82300366889228	19.453893349848272	19.618187285402243
9	27.435332220186186	14.08918865519686	18.592204612458207	39.88327451215874
10	16.23352908239479	26.504978329397037	31.279195252423293	25.982297335784878
11	37.78869343289496	20.98030677393308	22.03948913959052	19.191510653581442
12	24.77636885513934	23.540602405261055	28.64590827065848	23.037120468941126
13	29.72540167694372	19.289066927589165	24.99639132561868	25.98914006984844
14	23.461071950335786	19.747445292246166	24.722634497564457	32.068848259853596
15	25.324292881200943	27.42751057566285	21.68531554561896	25.56288099751725
16	25.846273664204773	25.56167598777334	23.781245323100215	24.81080502492167
17	24.30454000508616	25.73510783874444	24.97538055390726	24.98497160226213
18	25.00496537799364	24.725790027970824	26.01833847310509	24.25090612093045
19	25.80565306318204	24.647010550201383	24.938790616918887	24.6085457696977
20	25.85421357131509	24.38952913868534	24.58701304740389	25.169244242595685
21	26.978573746905717	24.014794432952733	24.045749385987435	24.960882434154115
22	26.22289086574801	24.425111925111924	24.53083512607322	24.821162083066845
23	24.749658079616214	24.240326305656232	25.215435276881625	25.794580337845925
24	24.97139291551972	24.94658880501353	24.94038777738698	25.141630502079764
25	24.984922081677993	24.57368851176465	24.995046691892608	25.44634271466475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	168.0
2	535.0
3	902.0
4	902.0
5	902.0
6	1799.0
7	2696.0
8	2696.0
9	2696.0
10	3109.0
11	3522.0
12	3522.0
13	3522.0
14	4387.5
15	5253.0
16	5253.0
17	5253.0
18	8560.0
19	11867.0
20	11867.0
21	11867.0
22	19651.5
23	27436.0
24	27436.0
25	27436.0
26	41382.0
27	55328.0
28	55328.0
29	55328.0
30	70590.5
31	85853.0
32	85853.0
33	85853.0
34	107311.5
35	128770.0
36	128770.0
37	128770.0
38	147686.0
39	166602.0
40	166602.0
41	166602.0
42	191578.0
43	216554.0
44	216554.0
45	216554.0
46	240369.5
47	264185.0
48	264185.0
49	264185.0
50	269995.5
51	275806.0
52	275806.0
53	275806.0
54	258034.0
55	240262.0
56	240262.0
57	240262.0
58	221315.0
59	202368.0
60	202368.0
61	202368.0
62	179601.0
63	156834.0
64	156834.0
65	156834.0
66	130849.0
67	104864.0
68	104864.0
69	104864.0
70	79858.0
71	54852.0
72	54852.0
73	54852.0
74	42700.0
75	30548.0
76	30548.0
77	30548.0
78	24575.5
79	18603.0
80	18603.0
81	18603.0
82	13191.5
83	7780.0
84	7780.0
85	7780.0
86	5753.5
87	3727.0
88	3727.0
89	3727.0
90	2506.5
91	1286.0
92	1286.0
93	1286.0
94	763.0
95	240.0
96	240.0
97	240.0
98	528.5
99	817.0
100	817.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008223990541443349
2	0.0035314782913256734
3	0.005466534889312344
4	0.016689863157635033
5	0.03884626120458241
6	0.04973095456825742
7	0.0718873526152048
8	0.0929794695332595
9	0.10192910629894786
10	0.12456926849539189
11	0.1260689373588316
12	0.14019485052413427
13	0.1283426288614659
14	0.13056794394915058
15	0.11982837983032456
16	0.11871572228648222
17	0.1305195675342009
18	0.13685687789260725
19	0.1401464741091846
20	0.1282458760315666
21	0.13705038355240592
22	0.1568847136817693
23	0.14735455993668495
24	0.1426620476865673
25	0.13767927694675158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2067123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.54645773317659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82377209735547	43.54104010811121
2	12.600480718456891	13.746231778540304
3	3.4497671222699324	5.645177295725973
4	1.374882995766687	2.9997998886660318
5	0.7061153115866674	1.9258044499105482
6	0.42659378812250115	1.3961508019835818
7	0.2717172391265042	1.0374849029572506
8	0.19778919659119634	0.8630960041552517
9	0.14897786811180613	0.731359348752545
>10	0.8253739736695189	8.244479759299724
>50	0.08034928650400061	3.059419404693797
>100	0.07815970077715419	9.00379862744513
>500	0.011481502857570708	4.236048521540134
>1k	0.00453919880415586	3.5701091082184973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2614	0.1264559486784289	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2202	0.1065248657191662	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2101	0.10163884780924985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.837641494966676E-5
2	0.0	0.0	0.0	0.0	4.837641494966676E-5
3	0.0	0.0	0.0	0.0	4.837641494966676E-5
4	0.0	0.0	0.0	0.0	4.837641494966676E-5
5	0.0	0.0	0.0	0.0	4.837641494966676E-5
6	0.0	0.0	0.0	0.0	4.837641494966676E-5
7	0.0	0.0	0.0	0.0	4.837641494966676E-5
8	0.0	0.0	0.0	0.0	4.837641494966676E-5
9	0.0	0.0	0.0	4.837641494966676E-5	4.837641494966676E-5
10	0.0	0.0	0.0	4.837641494966676E-5	4.837641494966676E-5
11	0.0	0.0	0.0	4.837641494966676E-5	4.837641494966676E-5
12	0.0	0.0	0.0	4.837641494966676E-5	2.4188207474833378E-4
13	0.0	0.0	0.0	4.837641494966676E-5	3.386349046476673E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATTCG	35	0.0021687883	16.287666	14
CGCCAGT	400	0.0	13.303849	18
CGCATCG	440	0.0	12.524228	13
GTACCGT	85	3.943005E-6	12.294698	6
CAAGACG	475	0.0	12.197917	4
ATCGCCA	440	0.0	12.093237	16
AACGCCG	460	0.0	11.976796	5
GTATCAA	2605	0.0	11.9182005	1
AAGACGG	465	0.0	11.848013	5
AATCGCT	145	9.640644E-11	11.794517	15
GGTATCA	1020	0.0	11.728462	1
AAGACCG	65	8.03362E-4	11.690878	5
CGAACGA	150	1.7644197E-10	11.402195	16
TAACGCC	485	0.0	11.358884	4
CGCAAGA	460	0.0	11.352358	2
GTATTAT	135	4.774847E-9	11.252704	1
GTATAAT	135	4.774847E-9	11.252704	1
ATAACGC	500	0.0	11.205373	3
TCGCCAG	475	0.0	11.2029705	17
GCCGCAT	485	0.0	11.165745	11
>>END_MODULE