FastQCFastQC Report
Thu 2 Feb 2017
SRR4062439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067123
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21860.10575084307997153No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21680.10488006761087754No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21450.1037674100670352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.021.0186581
GGTATCA16850.018.5443521
TAGATCG951.5981477E-518.5261235
TAGGACG8050.017.4905014
GTCCTAA6200.017.3911231
TAATACC1408.3815394E-817.2855344
TACCGTC6300.017.1109317
ATACCGT5950.017.0082266
CGTCGTA6100.016.95064210
TGTAGGA10400.016.9257642
GCATACG1906.184564E-1016.21035837
CCGTCGT6400.016.1560829
ACCGTCG6150.016.0973938
GTAGGAC10600.015.5666283
ATAACGC7350.015.5652163
ACTAGAC1704.492904E-815.5300013
CATACCG850.00298110615.5292499
AACGCAG38050.015.4952396
GTCGTAG6800.015.20572311
TAACGCC7250.015.1722554
Produced by FastQC (version 0.11.5)