##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2067123
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332406441222897	32.0	32.0	32.0	32.0	32.0
2	31.4340926979188	32.0	32.0	32.0	32.0	32.0
3	31.514458501018083	32.0	32.0	32.0	32.0	32.0
4	31.606694908817715	32.0	32.0	32.0	32.0	32.0
5	31.554024603277114	32.0	32.0	32.0	32.0	32.0
6	35.14773334726574	36.0	36.0	36.0	36.0	36.0
7	35.159953229682024	36.0	36.0	36.0	36.0	36.0
8	35.10697428261405	36.0	36.0	36.0	36.0	36.0
9	35.232063113806	36.0	36.0	36.0	36.0	36.0
10	35.10742418327308	36.0	36.0	36.0	36.0	36.0
11	35.239928151348515	36.0	36.0	36.0	36.0	36.0
12	35.143935314928044	36.0	36.0	36.0	36.0	36.0
13	35.19027314775173	36.0	36.0	36.0	36.0	36.0
14	35.157487967576195	36.0	36.0	36.0	36.0	36.0
15	35.11600374046441	36.0	36.0	36.0	36.0	36.0
16	35.12933192654719	36.0	36.0	36.0	36.0	36.0
17	35.102616535155384	36.0	36.0	36.0	36.0	36.0
18	35.114350234601424	36.0	36.0	36.0	36.0	36.0
19	35.099548502919276	36.0	36.0	36.0	36.0	36.0
20	35.0969274687573	36.0	36.0	36.0	36.0	36.0
21	35.09254698438361	36.0	36.0	36.0	36.0	36.0
22	35.08053657184406	36.0	36.0	36.0	36.0	36.0
23	35.04366068202037	36.0	36.0	36.0	36.0	36.0
24	35.019528591186884	36.0	36.0	36.0	36.0	36.0
25	34.99242086706984	36.0	36.0	36.0	36.0	36.0
26	34.95581927151892	36.0	36.0	36.0	32.0	36.0
27	34.93740333787588	36.0	36.0	36.0	32.0	36.0
28	34.92054367350177	36.0	36.0	36.0	32.0	36.0
29	34.90008093374221	36.0	36.0	36.0	32.0	36.0
30	34.88449115026053	36.0	36.0	36.0	32.0	36.0
31	34.88694044814943	36.0	36.0	36.0	32.0	36.0
32	34.856010987251366	36.0	36.0	36.0	32.0	36.0
33	34.84740433926767	36.0	36.0	36.0	32.0	36.0
34	34.834203866920355	36.0	36.0	36.0	32.0	36.0
35	34.81702588573587	36.0	36.0	36.0	32.0	36.0
36	34.794808049641944	36.0	36.0	36.0	32.0	36.0
37	34.787564165267376	36.0	36.0	36.0	32.0	36.0
38	34.75667340550127	36.0	36.0	36.0	32.0	36.0
39	34.74844119096928	36.0	36.0	36.0	32.0	36.0
40	34.75575522114553	36.0	36.0	36.0	32.0	36.0
41	34.718293009172655	36.0	36.0	36.0	32.0	36.0
42	34.707143213055055	36.0	36.0	36.0	32.0	36.0
43	34.69385517939668	36.0	36.0	36.0	32.0	36.0
44	34.6620234983598	36.0	36.0	36.0	32.0	36.0
45	34.63764710663081	36.0	36.0	36.0	32.0	36.0
46	34.63013086304008	36.0	36.0	36.0	32.0	36.0
47	34.620873068511166	36.0	36.0	36.0	32.0	36.0
48	34.57661445400201	36.0	36.0	36.0	32.0	36.0
49	34.56138507481171	36.0	36.0	36.0	32.0	36.0
50	34.065773541293865	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	39.0
22	153.0
23	436.0
24	1219.0
25	2860.0
26	5948.0
27	11130.0
28	18724.0
29	29098.0
30	42683.0
31	61742.0
32	92496.0
33	154320.0
34	347798.0
35	1298467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05794268533836	17.8442433499887	11.374534848783217	26.723279115889724
2	16.69224256791079	20.240584407730825	36.35853027261072	26.708642751747657
3	18.736254964660805	23.6978680379854	28.387216935721852	29.178660061631938
4	12.648654819916203	15.798010856645547	35.65553250148395	35.8978018219543
5	14.765062359617692	36.26494407928314	33.29289065043541	15.67710291066376
6	34.63337703363633	35.197739839679556	16.568349047704285	13.60053407897983
7	30.489525780517173	30.05152571956289	20.67385443439989	18.78509406552005
8	28.230782590102283	32.86388860266177	19.286225347983645	19.619103459252305
9	27.552254994018256	13.896028441461878	18.417675193977328	40.13404137054254
10	16.2153872798087	26.63736023449016	31.235103087721438	25.912149397979707
11	38.03121536551043	20.815016813223018	22.038746605789786	19.115021215476776
12	24.847916645501986	23.515291542883514	28.562838302316795	23.073953509297702
13	29.93077818784852	19.18226443225681	24.98690208565238	25.900055294242286
14	23.54804237580444	19.69360313827479	24.629400379174342	32.128954106746434
15	25.395295780657463	27.395660538826185	21.655895657878123	25.55314802263823
16	26.017561606155027	25.45963641254052	23.6913816933003	24.83142028800415
17	24.425397037331596	25.68376434300233	24.88623076614212	25.004607853523957
18	25.199081041621618	24.681018013925634	25.89226669143539	24.227634253017357
19	25.98524616096865	24.657458699845147	24.832242687058294	24.52505245212791
20	26.082482755017484	24.360621017713992	24.440296973136093	25.11659925413243
21	27.084455061454975	24.006892671602028	24.003070934821004	24.905581332121987
22	26.344924806119423	24.354719095090132	24.467145883433158	24.833210215357287
23	24.77573903439708	24.147813168350407	25.146931266305877	25.929516530946632
24	25.15215591912044	24.82106773520492	24.911773513235545	25.115002832439092
25	25.185535645435706	24.374553425219496	25.043308985483687	25.396601943861107
26	24.764128694809163	25.32181200634892	25.33772784686736	24.576331451974557
27	25.71003273632	24.59432747833583	24.5118940672616	25.183745718082573
28	24.73955347601473	24.72692723171287	25.284852425327376	25.248666866945022
29	24.95603793291449	24.562060409564403	24.94326655936778	25.53863509815333
30	24.490705197513645	24.984338135660046	25.523928668008626	25.001027998817683
31	25.540279896261612	24.572171080288886	24.380697229918102	25.5068517935314
32	25.17653763225507	24.71860648834153	24.38142287614235	25.723433003261054
33	24.755324187288323	24.39564554213755	25.09536200796953	25.753668262604595
34	25.426740450374748	24.57328373783273	25.19148594447452	24.808489867318006
35	25.831022150109114	24.428106116568777	25.08317115140222	24.657700581919894
36	24.547112097344957	25.01413800726904	24.88352168690494	25.55522820848106
37	25.650191111027258	24.714736375145552	24.560850999190663	25.074221514636523
38	24.91574037926142	24.592924562302294	25.03015060061738	25.46118445781891
39	25.670460828891166	24.32167800367951	24.466323484379014	25.541537683050308
40	25.874028782999364	24.42467139110735	25.01873376668926	24.682566059204024
41	24.75382451842488	24.888552834059706	25.493451526590338	24.864171120925075
42	25.912826667789002	25.10266684662693	24.88845608122981	24.096050404354262
43	25.09570064287418	24.123770090120424	25.20720827933316	25.573320987672236
44	24.678212181858555	24.758323525015204	24.852270522847455	25.71119377027879
45	24.79049384095673	24.91225727738504	24.98356411302085	25.31368476863738
46	24.694103538206036	24.38289775973443	25.19441290567896	25.72858579638057
47	25.05494356643854	24.168638410620375	25.451969188063977	25.324448834877106
48	25.454073124821306	24.947959071617895	24.556303616185392	25.0416641873754
49	24.740811262803422	25.00417246578941	24.843707897401366	25.411308374005802
50	24.593685901157166	25.29398390030574	24.724641046480127	25.387689152056968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	20.0
2	23.0
3	64.5
4	106.0
5	129.0
6	152.0
7	159.0
8	166.0
9	238.0
10	310.0
11	447.5
12	585.0
13	1055.0
14	1525.0
15	2101.5
16	2678.0
17	3157.5
18	3637.0
19	3965.0
20	4293.0
21	5122.0
22	5951.0
23	6794.5
24	7638.0
25	9552.5
26	11467.0
27	14658.5
28	17850.0
29	21456.5
30	25063.0
31	28515.5
32	31968.0
33	38045.0
34	44122.0
35	51768.0
36	59414.0
37	69137.5
38	78861.0
39	84029.0
40	89197.0
41	96211.5
42	103226.0
43	106669.5
44	110113.0
45	120324.0
46	130535.0
47	139202.0
48	147869.0
49	154468.5
50	161068.0
51	156077.0
52	151086.0
53	148409.5
54	145733.0
55	147824.5
56	149916.0
57	146217.0
58	142518.0
59	130713.0
60	118908.0
61	104574.5
62	90241.0
63	78792.0
64	67343.0
65	57628.5
66	47914.0
67	42349.0
68	36784.0
69	33354.0
70	29924.0
71	23186.5
72	16449.0
73	14542.0
74	12635.0
75	9387.0
76	6139.0
77	5542.5
78	4946.0
79	4253.0
80	3560.0
81	2832.5
82	2105.0
83	1786.5
84	1468.0
85	1207.0
86	946.0
87	718.5
88	491.0
89	310.0
90	129.0
91	80.5
92	32.0
93	20.0
94	8.0
95	7.0
96	6.0
97	6.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015577205613792697
2	0.0035314782913256734
3	6.288933943456678E-4
4	9.675282989933352E-5
5	0.0
6	6.288933943456678E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.675282989933352E-5
47	9.675282989933352E-5
48	0.0
49	0.0
50	1.4512924484900029E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2067123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.61052807272924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64821812509317	45.08927688113449
2	12.950576887972195	14.662779929491768
3	3.449711265066824	5.858699292416273
4	1.3660805267039855	3.0933816002633887
5	0.6743821511476639	1.908856484964618
6	0.41781899862830263	1.4191772490700285
7	0.2559673666039273	1.0143313454983944
8	0.19272404588014228	0.8728168007670201
9	0.13654704486588262	0.695700028496544
>10	0.7540617418792153	7.719614325601919
>50	0.07329034900073253	2.8805889962023135
>100	0.06652811495166044	7.911536098144658
>500	0.010312230882607446	3.813791552350839
>1k	0.0037811513236227262	3.059449415597828
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2186	0.10575084307997153	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2168	0.10488006761087754	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2145	0.1037674100670352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4512924484900029E-4
2	0.0	0.0	0.0	0.0	1.4512924484900029E-4
3	0.0	0.0	0.0	0.0	1.4512924484900029E-4
4	0.0	0.0	0.0	0.0	1.4512924484900029E-4
5	0.0	0.0	0.0	0.0	1.4512924484900029E-4
6	0.0	0.0	0.0	0.0	1.9350565979866705E-4
7	0.0	0.0	0.0	0.0	2.9025848969800057E-4
8	0.0	0.0	0.0	0.0	2.9025848969800057E-4
9	0.0	0.0	0.0	0.0	2.9025848969800057E-4
10	0.0	0.0	0.0	0.0	2.9025848969800057E-4
11	0.0	0.0	0.0	0.0	2.9025848969800057E-4
12	0.0	0.0	0.0	0.0	3.386349046476673E-4
13	0.0	0.0	0.0	0.0	5.321405644463344E-4
14	0.0	0.0	0.0	0.0	5.321405644463344E-4
15	0.0	0.0	0.0	0.0	5.321405644463344E-4
16	0.0	0.0	0.0	0.0	5.805169793960011E-4
17	0.0	0.0	0.0	0.0	5.805169793960011E-4
18	0.0	0.0	0.0	0.0	6.772698092953346E-4
19	0.0	0.0	0.0	0.0	6.772698092953346E-4
20	0.0	0.0	0.0	0.0	7.256462242450014E-4
21	0.0	0.0	0.0	0.0	7.740226391946682E-4
22	0.0	0.0	0.0	9.675282989933352E-5	7.740226391946682E-4
23	0.0	0.0	0.0	1.9350565979866705E-4	7.740226391946682E-4
24	0.0	0.0	0.0	5.805169793960011E-4	7.740226391946682E-4
25	0.0	0.0	0.0	6.772698092953346E-4	7.740226391946682E-4
26	0.0	0.0	0.0	8.707754690940017E-4	8.223990541443349E-4
27	0.0	0.0	0.0	0.0011610339587920023	9.191518840436685E-4
28	0.0	0.0	0.0	0.0037733603660740074	9.191518840436685E-4
29	0.0	0.0	0.0	0.00880450752083935	9.191518840436685E-4
30	0.0	0.0	0.0	0.019011931075219035	9.675282989933351E-4
31	0.0	0.0	0.0	0.044361172508844415	9.675282989933351E-4
32	0.0	0.0	0.0	0.07425779694773847	9.675282989933351E-4
33	0.0	0.0	0.0	0.10942745061614621	9.675282989933351E-4
34	0.0	0.0	0.0	0.14909611087487296	9.675282989933351E-4
35	0.0	0.0	0.0	0.1932637777239187	9.675282989933351E-4
36	0.0	0.0	0.0	0.2613777699730495	9.675282989933351E-4
37	0.0	0.0	0.0	0.36025916213016834	9.675282989933351E-4
38	0.0	0.0	0.0	0.49721279285267495	0.0010642811288926687
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2900	0.0	21.018658	1
GGTATCA	1685	0.0	18.544352	1
TAGATCG	95	1.5981477E-5	18.526123	5
TAGGACG	805	0.0	17.490501	4
GTCCTAA	620	0.0	17.391123	1
TAATACC	140	8.3815394E-8	17.285534	4
TACCGTC	630	0.0	17.110931	7
ATACCGT	595	0.0	17.008226	6
CGTCGTA	610	0.0	16.950642	10
TGTAGGA	1040	0.0	16.925764	2
GCATACG	190	6.184564E-10	16.210358	37
CCGTCGT	640	0.0	16.156082	9
ACCGTCG	615	0.0	16.097393	8
GTAGGAC	1060	0.0	15.566628	3
ATAACGC	735	0.0	15.565216	3
ACTAGAC	170	4.492904E-8	15.530001	3
CATACCG	85	0.002981106	15.529249	9
AACGCAG	3805	0.0	15.495239	6
GTCGTAG	680	0.0	15.205723	11
TAACGCC	725	0.0	15.172255	4
>>END_MODULE