FastQCFastQC Report
Thu 2 Feb 2017
SRR4062438_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062438_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755402
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30310.17266700163267446No Hit
TATCAACGCAGAGTACTTTTTTTTT19780.11268074207503467No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17820.10151520848215964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG602.5593765E-514.25545618
CGCGCCA903.637797E-813.72277810
TCGCGTA1205.0931703E-1113.45887859
TAAGGCG604.0979858E-412.6646525
GTCTTAA1352.7284841E-1112.659961
CGCGTAA1301.9826984E-1012.42358110
CGATAAC1155.322363E-912.39180710
GTGCCGA701.0906418E-412.21478111
GTCCTAG1701.8189894E-1211.7290811
GTATCAA24950.011.5690211
CGAACGA1157.0567694E-811.56733516
TAATACT1900.011.4978434
GGTTCTA4450.011.31571113
CCGACCA2200.011.2277289
TATACCG855.3321637E-511.1746935
GTCCTAA2900.011.1320331
GATAACG1201.274675E-711.08378211
CTATTCC3700.011.038474
GTTCTAA951.3683688E-510.9941761
ATTCCGA1303.26836E-810.9610456
Produced by FastQC (version 0.11.5)