##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062438_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1755402
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237299490373147	32.0	32.0	32.0	32.0	32.0
2	30.902372220152422	32.0	32.0	32.0	32.0	32.0
3	30.894131942426863	32.0	32.0	32.0	32.0	32.0
4	30.968574149966788	32.0	32.0	32.0	32.0	32.0
5	30.762018044869496	32.0	32.0	32.0	32.0	32.0
6	34.434055561062365	36.0	36.0	36.0	32.0	36.0
7	34.32852873586791	36.0	36.0	36.0	32.0	36.0
8	34.332596180248174	36.0	36.0	36.0	32.0	36.0
9	34.4901521133051	36.0	36.0	36.0	32.0	36.0
10	34.17039230899817	36.0	36.0	36.0	32.0	36.0
11	34.46133478257402	36.0	36.0	36.0	32.0	36.0
12	34.248120373566856	36.0	36.0	36.0	32.0	36.0
13	34.3730695305121	36.0	36.0	36.0	32.0	36.0
14	34.280415540144084	36.0	36.0	36.0	32.0	36.0
15	34.21750915174986	36.0	36.0	36.0	32.0	36.0
16	34.2227786000016	36.0	36.0	36.0	32.0	36.0
17	34.14171568677716	36.0	36.0	36.0	32.0	36.0
18	34.15527497405153	36.0	36.0	36.0	32.0	36.0
19	34.1495458020442	36.0	36.0	36.0	32.0	36.0
20	34.13927977750965	36.0	36.0	36.0	32.0	36.0
21	34.123226474619486	36.0	36.0	36.0	32.0	36.0
22	34.08275540303588	36.0	36.0	36.0	32.0	36.0
23	34.046299935855146	36.0	36.0	36.0	32.0	36.0
24	34.02250766491095	36.0	36.0	36.0	32.0	36.0
25	33.57658872440615	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	15.0
4	64.0
5	140.0
6	308.0
7	82.0
8	259.0
9	302.0
10	210.0
11	61.0
12	115.0
13	98.0
14	230.0
15	332.0
16	522.0
17	638.0
18	904.0
19	1191.0
20	1875.0
21	2886.0
22	4279.0
23	6547.0
24	9490.0
25	13709.0
26	19177.0
27	24889.0
28	34432.0
29	45544.0
30	59708.0
31	81922.0
32	117776.0
33	172664.0
34	393393.0
35	761640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.60878368600784	17.088182109255982	11.129500345818895	26.173533858917285
2	17.29271393999608	19.46817504409372	36.623195803462785	26.615915212447415
3	19.446121873241072	23.01103153135703	27.991229009668828	29.55161758573307
4	13.086211690276942	14.790663913653852	35.098406534303265	37.024717861765936
5	15.081100456519522	36.05090953436832	33.28079170320931	15.587198305902842
6	35.1360377889713	34.60377547752005	16.38825341134604	13.871933322162608
7	30.987601539119282	29.858001995154627	20.314721390907795	18.8396750748183
8	28.536998804365858	32.48242041506807	19.171882242865966	19.808698537700106
9	27.534119646130733	14.19291748277268	18.337681147024526	39.93528172407206
10	16.20971596281886	26.444738736210777	31.20199935894584	26.14354594202452
11	37.97887011462655	20.917491470593937	21.70045159369571	19.4031868210838
12	24.86309996440599	23.308885268643504	28.338763701411896	23.48925106553861
13	29.694613298899576	19.236670130575288	25.06972494436263	25.99899162616251
14	23.687033833486478	19.3334941762873	24.61068305211483	32.3687889381114
15	25.36521296271477	27.11133441958585	21.832534992862556	25.690917624836818
16	26.032518192394555	25.596528047083517	23.38686497707416	24.98408878344777
17	24.38494786301679	25.728782740325766	24.798119623605317	25.08814977305212
18	25.169520915184783	24.763628979634674	25.650077345343536	24.416772759837006
19	25.774234378841363	24.780234541979183	25.011137293018898	24.434393786160552
20	26.0009787068522	24.0429946604583	24.657310016231936	25.29871661645756
21	26.825528740110098	23.949892110152028	24.333253288173413	24.89132586156446
22	26.08547566383672	24.107329802607595	24.599969306305386	25.207225227250298
23	24.755191380272244	24.056789438916784	25.227695119572026	25.96032406123895
24	25.030146287928133	24.618365677609834	24.966944609547102	25.384543424914924
25	25.025867546752668	24.508060293344254	24.95302772932564	25.513044430577438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	181.0
1	181.0
2	437.0
3	693.0
4	693.0
5	693.0
6	1606.5
7	2520.0
8	2520.0
9	2520.0
10	2838.0
11	3156.0
12	3156.0
13	3156.0
14	3810.5
15	4465.0
16	4465.0
17	4465.0
18	7201.5
19	9938.0
20	9938.0
21	9938.0
22	16070.5
23	22203.0
24	22203.0
25	22203.0
26	32963.0
27	43723.0
28	43723.0
29	43723.0
30	55824.0
31	67925.0
32	67925.0
33	67925.0
34	85518.0
35	103111.0
36	103111.0
37	103111.0
38	120899.5
39	138688.0
40	138688.0
41	138688.0
42	160008.0
43	181328.0
44	181328.0
45	181328.0
46	203055.0
47	224782.0
48	224782.0
49	224782.0
50	231540.0
51	238298.0
52	238298.0
53	238298.0
54	223638.5
55	208979.0
56	208979.0
57	208979.0
58	193265.5
59	177552.0
60	177552.0
61	177552.0
62	156774.0
63	135996.0
64	135996.0
65	135996.0
66	113134.5
67	90273.0
68	90273.0
69	90273.0
70	68712.5
71	47152.0
72	47152.0
73	47152.0
74	36971.5
75	26791.0
76	26791.0
77	26791.0
78	21561.5
79	16332.0
80	16332.0
81	16332.0
82	11377.5
83	6423.0
84	6423.0
85	6423.0
86	4726.5
87	3030.0
88	3030.0
89	3030.0
90	1988.0
91	946.0
92	946.0
93	946.0
94	576.5
95	207.0
96	207.0
97	207.0
98	458.5
99	710.0
100	710.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00843111720278318
2	0.002848350406345669
3	0.003987690568883937
4	0.01333027990169773
5	0.03435110590052877
6	0.046485078631561316
7	0.06562599336220422
8	0.08664681936103524
9	0.09382466238502633
10	0.1162126965789033
11	0.11712416870893391
12	0.13091018467564694
13	0.11871924493648749
14	0.12185243038346771
15	0.1123959070344001
16	0.10949058961992751
17	0.12168152935908697
18	0.1268085600905092
19	0.13005567955374325
20	0.11780777280645688
21	0.12675159308238226
22	0.14805725412184786
23	0.1367208195045921
24	0.13011264656187016
25	0.12760609820428598
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1755402.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.63812227772331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82893569096613	43.07065027305794
2	12.707818979520521	13.886627345724307
3	3.6793058726986914	6.030910925089697
4	1.5138630658305845	3.308585412103223
5	0.7996868327550782	2.184669347597862
6	0.4954025204995071	1.6240718095046638
7	0.34349803900881193	1.3137661500265145
8	0.2642369376335431	1.154992808697014
9	0.18498065765267432	0.9096296212656445
>10	1.0256822752398038	9.969470418211449
>50	0.07421406236450721	2.8283146551499527
>100	0.07084723516031784	8.307764976904265
>500	0.008698272232773772	3.233982517174315
>1k	0.0028295584371673716	2.176563739493087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3031	0.17266700163267446	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1978	0.11268074207503467	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1782	0.10151520848215964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1393401625382676E-4
2	0.0	0.0	0.0	0.0	1.1393401625382676E-4
3	0.0	0.0	0.0	0.0	1.1393401625382676E-4
4	0.0	0.0	0.0	0.0	1.1393401625382676E-4
5	0.0	5.696700812691338E-5	0.0	0.0	1.1393401625382676E-4
6	0.0	5.696700812691338E-5	0.0	0.0	1.7090102438074013E-4
7	0.0	5.696700812691338E-5	0.0	0.0	1.7090102438074013E-4
8	0.0	5.696700812691338E-5	0.0	5.696700812691338E-5	1.7090102438074013E-4
9	0.0	5.696700812691338E-5	0.0	1.7090102438074013E-4	1.7090102438074013E-4
10	0.0	5.696700812691338E-5	0.0	1.7090102438074013E-4	1.7090102438074013E-4
11	0.0	5.696700812691338E-5	0.0	1.7090102438074013E-4	1.7090102438074013E-4
12	0.0	5.696700812691338E-5	0.0	2.2786803250765352E-4	3.4180204876148025E-4
13	0.0	5.696700812691338E-5	0.0	2.2786803250765352E-4	3.9876905688839367E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCCG	60	2.5593765E-5	14.255456	18
CGCGCCA	90	3.637797E-8	13.722778	10
TCGCGTA	120	5.0931703E-11	13.4588785	9
TAAGGCG	60	4.0979858E-4	12.664652	5
GTCTTAA	135	2.7284841E-11	12.65996	1
CGCGTAA	130	1.9826984E-10	12.423581	10
CGATAAC	115	5.322363E-9	12.391807	10
GTGCCGA	70	1.0906418E-4	12.214781	11
GTCCTAG	170	1.8189894E-12	11.729081	1
GTATCAA	2495	0.0	11.569021	1
CGAACGA	115	7.0567694E-8	11.567335	16
TAATACT	190	0.0	11.497843	4
GGTTCTA	445	0.0	11.315711	13
CCGACCA	220	0.0	11.227728	9
TATACCG	85	5.3321637E-5	11.174693	5
GTCCTAA	290	0.0	11.132033	1
GATAACG	120	1.274675E-7	11.083782	11
CTATTCC	370	0.0	11.03847	4
GTTCTAA	95	1.3683688E-5	10.994176	1
ATTCCGA	130	3.26836E-8	10.961045	6
>>END_MODULE