FastQCFastQC Report
Thu 2 Feb 2017
SRR4062438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755402
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17720.1009455384008905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.022.29981
ACTCCGT1352.5993359E-919.555916
TACCGTC4450.018.2919457
ATACCGT4400.017.5003176
GGTATCA13050.017.0300391
ATACGAA5950.017.00822840
GGCGTTA4450.016.80881542
ACCGTCG4650.016.5589688
GTATTCG800.001986321316.503121
AACGCAG35200.016.4377986
TACGAAT6300.016.41252941
CGTCGTA4750.016.2103610
CTAGCGG6250.016.19183229
GTAATAC1501.8379978E-716.1340853
TCGTTTA5200.016.07675730
CGCAATA6050.015.99983536
GTTATAT1958.894858E-1015.7978581
CGAATGC6550.015.78609543
TCTAGCG6400.015.4685928
GTATAGA2001.2732926E-915.4029111
Produced by FastQC (version 0.11.5)