##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1755402
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305913403311607	32.0	32.0	32.0	32.0	32.0
2	31.42054640475515	32.0	32.0	32.0	32.0	32.0
3	31.505164628956788	32.0	32.0	32.0	32.0	32.0
4	31.609328233646767	32.0	32.0	32.0	32.0	32.0
5	31.54012926953484	32.0	32.0	32.0	32.0	32.0
6	35.13790630294371	36.0	36.0	36.0	36.0	36.0
7	35.154649476302296	36.0	36.0	36.0	36.0	36.0
8	35.094741261545785	36.0	36.0	36.0	36.0	36.0
9	35.232522806741706	36.0	36.0	36.0	36.0	36.0
10	35.10286247822436	36.0	36.0	36.0	36.0	36.0
11	35.23415320251429	36.0	36.0	36.0	36.0	36.0
12	35.1537733237173	36.0	36.0	36.0	36.0	36.0
13	35.190778522526465	36.0	36.0	36.0	36.0	36.0
14	35.156435392007076	36.0	36.0	36.0	36.0	36.0
15	35.11387534023546	36.0	36.0	36.0	36.0	36.0
16	35.13275648540904	36.0	36.0	36.0	36.0	36.0
17	35.103493103004325	36.0	36.0	36.0	36.0	36.0
18	35.116567601039534	36.0	36.0	36.0	36.0	36.0
19	35.10734521209387	36.0	36.0	36.0	36.0	36.0
20	35.09802711857455	36.0	36.0	36.0	36.0	36.0
21	35.09958915393739	36.0	36.0	36.0	36.0	36.0
22	35.080914229333224	36.0	36.0	36.0	36.0	36.0
23	35.04329321716621	36.0	36.0	36.0	36.0	36.0
24	35.021120518263054	36.0	36.0	36.0	36.0	36.0
25	34.99739034135771	36.0	36.0	36.0	36.0	36.0
26	34.95895925833513	36.0	36.0	36.0	32.0	36.0
27	34.938047808991904	36.0	36.0	36.0	32.0	36.0
28	34.932004179099714	36.0	36.0	36.0	32.0	36.0
29	34.90976710747738	36.0	36.0	36.0	32.0	36.0
30	34.90010835124946	36.0	36.0	36.0	32.0	36.0
31	34.89891375308903	36.0	36.0	36.0	32.0	36.0
32	34.865584635314306	36.0	36.0	36.0	32.0	36.0
33	34.85267306292234	36.0	36.0	36.0	32.0	36.0
34	34.83166533933538	36.0	36.0	36.0	32.0	36.0
35	34.816503000452315	36.0	36.0	36.0	32.0	36.0
36	34.7901016405359	36.0	36.0	36.0	32.0	36.0
37	34.79103988715975	36.0	36.0	36.0	32.0	36.0
38	34.757265287381465	36.0	36.0	36.0	32.0	36.0
39	34.751075821948476	36.0	36.0	36.0	32.0	36.0
40	34.74781218205288	36.0	36.0	36.0	32.0	36.0
41	34.71712063675443	36.0	36.0	36.0	32.0	36.0
42	34.698531162662455	36.0	36.0	36.0	32.0	36.0
43	34.69120406607717	36.0	36.0	36.0	32.0	36.0
44	34.65566178003671	36.0	36.0	36.0	32.0	36.0
45	34.626443971238494	36.0	36.0	36.0	32.0	36.0
46	34.61631352818329	36.0	36.0	36.0	32.0	36.0
47	34.6038468681248	36.0	36.0	36.0	32.0	36.0
48	34.564275305599516	36.0	36.0	36.0	32.0	36.0
49	34.5464839392914	36.0	36.0	36.0	32.0	36.0
50	34.046869605936415	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	41.0
22	125.0
23	411.0
24	1020.0
25	2420.0
26	4934.0
27	9159.0
28	15803.0
29	24553.0
30	36064.0
31	52557.0
32	78061.0
33	132506.0
34	300863.0
35	1096876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00770776582477	17.72415793171152	11.62380997690747	26.644324325556244
2	16.761302297176197	19.955575279619946	36.23861965477697	27.044502768426888
3	18.792576008750192	23.312255396236733	28.399273096007153	29.49589549900592
4	12.778355677743736	15.320969945277369	35.29985792395799	36.60081645302091
5	14.790173418966141	36.314473835622834	33.15793191531057	15.737420830100458
6	35.25106700057195	34.82506431626512	16.151699795145007	13.77216888801792
7	30.87465485094289	29.956004354560584	20.32452983677234	18.84481095772419
8	28.54098377465675	32.65121037802167	18.963633401351938	19.84417244596964
9	27.57117742830417	13.959708374491997	18.312272630428815	40.15684156677502
10	16.07620362743121	26.50036857654258	31.27551409876484	26.147913697261366
11	38.179459747681726	20.872597843684808	21.639487707089316	19.308454701544147
12	24.903412437720817	23.33260415562931	28.26913721187512	23.49484619477476
13	29.84296474539735	19.0964804643039	25.092884706750933	25.967670083547812
14	23.70556715783621	19.210756282606493	24.641933870418285	32.44174268913901
15	25.46698704912037	27.110770068622458	21.758548754074567	25.663694128182605
16	26.111113010011383	25.44744736533284	23.441923844224853	24.999515780430922
17	24.531588775676454	25.646490091728275	24.71325656459318	25.108664568002087
18	25.30144092350356	24.528113788180715	25.74789136619418	24.422553922121544
19	25.87566836542285	24.623818361833926	25.051697559875173	24.44881571286805
20	26.13623546059535	23.896121800020737	24.68351978635093	25.284122953032977
21	26.910189233007596	23.847073206023463	24.26219179424428	24.98054576672466
22	26.154635804220344	24.02555084248508	24.69833120846393	25.121482144830644
23	24.793010375970862	23.88592470556602	25.360344809906792	25.96072010855633
24	25.121254276798133	24.47188735115945	24.995129320805148	25.411729051237263
25	25.08183310717431	24.21758662688091	25.171214342925442	25.52936592301934
26	24.83414055583849	24.869460100877177	25.399652045514358	24.89674729776997
27	25.517345884304564	24.451094393193127	24.733650753502616	25.297908968999693
28	24.967158519814834	24.376182777506237	25.3123216220558	25.34433708062313
29	24.891677234046675	24.46305746489978	25.292497103227635	25.35276819782591
30	24.81442997102658	24.701806195959673	25.449555144633536	25.03420868838021
31	25.498945540679568	24.38051227012388	24.554318611919093	25.56622357727746
32	25.174347528372422	24.473254559354494	24.60182909669694	25.75056881557615
33	24.81705045340042	24.21069361889755	25.133046447480407	25.839209480221626
34	25.47325342001433	24.363479134693932	25.171157375917314	24.992110069374423
35	25.87105403776457	24.200724392475344	25.112025621481575	24.816195948278512
36	24.764697772931786	24.76731825530562	24.918052958809437	25.54993101295316
37	25.737979106780102	24.482141412622294	24.68186774311525	25.098011737482356
38	24.964993773506013	24.26355900243933	25.135154226781104	25.636292997273557
39	25.54394947709983	24.3297546658828	24.625698273102113	25.50059758391525
40	25.692804269335458	24.297340438258587	25.113563730701	24.896291561704952
41	24.784237456719318	24.693090243716252	25.4939324439644	25.02873985560003
42	25.853280331228973	24.723339725031646	24.924262362695266	24.499117581044114
43	24.936909038499444	23.890596000232424	25.382049240003145	25.790445721264987
44	24.701350459894655	24.521619549254243	24.986242467537352	25.790787523313746
45	24.789877190523878	24.662043224287086	25.046570529143754	25.50150905604528
46	24.714053451126183	24.290274912014258	25.27700270821774	25.718668928641826
47	24.888900029110157	24.24067207435794	25.49673835209163	25.373689544440275
48	25.47131654173802	24.708756170951155	24.732568380348205	25.08735890696262
49	24.669406021757993	24.80681052363534	24.959710060550265	25.564073394056404
50	24.54407853248346	25.034678685952667	24.898413524886905	25.522829256676964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	38.0
2	46.0
3	127.0
4	208.0
5	240.0
6	272.0
7	259.0
8	246.0
9	321.5
10	397.0
11	607.0
12	817.0
13	1429.0
14	2041.0
15	2672.0
16	3303.0
17	3615.0
18	3927.0
19	4118.5
20	4310.0
21	4948.0
22	5586.0
23	6220.5
24	6855.0
25	8218.5
26	9582.0
27	11912.5
28	14243.0
29	16641.0
30	19039.0
31	21997.0
32	24955.0
33	29594.0
34	34233.0
35	39712.5
36	45192.0
37	53125.0
38	61058.0
39	67003.0
40	72948.0
41	80544.0
42	88140.0
43	91566.0
44	94992.0
45	103929.0
46	112866.0
47	119967.0
48	127068.0
49	131215.5
50	135363.0
51	132386.5
52	129410.0
53	128034.5
54	126659.0
55	127002.5
56	127346.0
57	123890.5
58	120435.0
59	111264.0
60	102093.0
61	90129.5
62	78166.0
63	68738.0
64	59310.0
65	50920.0
66	42530.0
67	37484.0
68	32438.0
69	29259.5
70	26081.0
71	20412.5
72	14744.0
73	13014.5
74	11285.0
75	8463.0
76	5641.0
77	4980.5
78	4320.0
79	3668.0
80	3016.0
81	2366.0
82	1716.0
83	1465.0
84	1214.0
85	990.0
86	766.0
87	551.5
88	337.0
89	228.5
90	120.0
91	74.0
92	28.0
93	22.0
94	16.0
95	9.5
96	3.0
97	5.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01646346534867797
2	0.004158591593264677
3	6.836040975229606E-4
4	2.2786803250765355E-4
5	0.0
6	7.975381137767873E-4
7	5.6967008126913386E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.2786803250765355E-4
47	5.6967008126913386E-5
48	0.0
49	5.6967008126913386E-5
50	5.6967008126913386E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1755402.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.53698610722473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.55958783449353	45.77618899929779
2	12.54419058756398	14.435098391260956
3	3.4777200588718102	6.002925921363723
4	1.4318825258153183	3.2954481998005525
5	0.7614576695111949	2.190598967595267
6	0.4779010809375903	1.649819271272029
7	0.3039419096061653	1.2241531001289303
8	0.2212454261278031	1.0183836007521936
9	0.17776429973830396	0.9205219839962994
>10	0.9107850826184372	9.237773168703166
>50	0.0643502217833443	2.5729619767147374
>100	0.060116652872515934	7.36457396832888
>500	0.0071657011464867585	2.8467103220163255
>1k	0.0018909489136562276	1.4648421287691273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1772	0.1009455384008905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1393401625382676E-4
2	0.0	0.0	0.0	0.0	1.1393401625382676E-4
3	0.0	0.0	0.0	0.0	1.1393401625382676E-4
4	0.0	0.0	0.0	0.0	1.1393401625382676E-4
5	0.0	5.696700812691338E-5	0.0	0.0	1.1393401625382676E-4
6	5.696700812691338E-5	5.696700812691338E-5	0.0	0.0	1.1393401625382676E-4
7	5.696700812691338E-5	1.7090102438074013E-4	0.0	5.696700812691338E-5	1.1393401625382676E-4
8	5.696700812691338E-5	1.7090102438074013E-4	0.0	5.696700812691338E-5	1.1393401625382676E-4
9	5.696700812691338E-5	1.7090102438074013E-4	0.0	1.1393401625382676E-4	1.1393401625382676E-4
10	5.696700812691338E-5	1.7090102438074013E-4	0.0	1.1393401625382676E-4	1.1393401625382676E-4
11	5.696700812691338E-5	1.7090102438074013E-4	0.0	1.7090102438074013E-4	1.1393401625382676E-4
12	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
13	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
14	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
15	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
16	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
17	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
18	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
19	5.696700812691338E-5	1.7090102438074013E-4	0.0	2.2786803250765352E-4	2.2786803250765352E-4
20	5.696700812691338E-5	1.7090102438074013E-4	0.0	3.4180204876148025E-4	2.2786803250765352E-4
21	5.696700812691338E-5	1.7090102438074013E-4	0.0	3.4180204876148025E-4	2.2786803250765352E-4
22	5.696700812691338E-5	1.7090102438074013E-4	0.0	5.696700812691338E-4	2.2786803250765352E-4
23	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.0014811422112997478	2.2786803250765352E-4
24	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.0022217133169496217	2.2786803250765352E-4
25	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.002677449381964929	2.2786803250765352E-4
26	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.003019251430726409	3.4180204876148025E-4
27	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.0042725256095185035	3.9876905688839367E-4
28	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.008146282162148613	3.9876905688839367E-4
29	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.016406498340551055	3.9876905688839367E-4
30	5.696700812691338E-5	1.7090102438074013E-4	0.0	0.03178759053481767	3.9876905688839367E-4
31	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.07109482614238789	4.5573606501530704E-4
32	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.11695326768455316	4.5573606501530704E-4
33	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.1694768491775673	4.5573606501530704E-4
34	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.22336763886562735	4.5573606501530704E-4
35	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.2856895457564706	4.5573606501530704E-4
36	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.3819637894909542	4.5573606501530704E-4
37	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.5141272483453933	4.5573606501530704E-4
38	1.1393401625382676E-4	1.7090102438074013E-4	0.0	0.6913516106282208	4.5573606501530704E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2605	0.0	22.2998	1
ACTCCGT	135	2.5993359E-9	19.55591	6
TACCGTC	445	0.0	18.291945	7
ATACCGT	440	0.0	17.500317	6
GGTATCA	1305	0.0	17.030039	1
ATACGAA	595	0.0	17.008228	40
GGCGTTA	445	0.0	16.808815	42
ACCGTCG	465	0.0	16.558968	8
GTATTCG	80	0.0019863213	16.50312	1
AACGCAG	3520	0.0	16.437798	6
TACGAAT	630	0.0	16.412529	41
CGTCGTA	475	0.0	16.21036	10
CTAGCGG	625	0.0	16.191832	29
GTAATAC	150	1.8379978E-7	16.134085	3
TCGTTTA	520	0.0	16.076757	30
CGCAATA	605	0.0	15.999835	36
GTTATAT	195	8.894858E-10	15.797858	1
CGAATGC	655	0.0	15.786095	43
TCTAGCG	640	0.0	15.46859	28
GTATAGA	200	1.2732926E-9	15.402911	1
>>END_MODULE