FastQCFastQC Report
Thu 2 Feb 2017
SRR4062437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547813
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG78321.4296849472356443No Hit
TCCATGTACTCTGCGTTGATACCAC63821.164996084430271No Hit
GAGTACATGGAAGCAGTGGTATCAA32000.5841409386049619No Hit
CATGTACTCTGCGTTGATACCACTG27020.4932340050345647No Hit
GCTTCCATGTACTCTGCGTTGATAC22670.4138273461929527No Hit
CCCATGTACTCTGCGTTGATACCAC20670.3773185375301426No Hit
CATGGAAGCAGTGGTATCAACGCAG17340.3165313711065637No Hit
GTACATGGGAAGCAGTGGTATCAAC17310.3159837389766216No Hit
GCGTTGATACCACTGCTTCCATGTA16390.29918968699172893No Hit
GTATCAACGCAGAGTACATGGAAGC14240.25994271767920807No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12430.22690224583936489No Hit
ACGCAGAGTACATGGAAGCAGTGGT11310.20645731298819123No Hit
ACTCTGCGTTGATACCACTGCTTCC11010.20098099168876973No Hit
CAGTGGTATCAACGCAGAGTACATG10900.19897300721231514No Hit
GTATCAACGCAGAGTACTTTTTTTT10740.19605230251929034No Hit
TATCAACGCAGAGTACATGGAAGCA9910.18090114692422413No Hit
TATCAACGCAGAGTACTTTTTTTTT8700.15881331768322404No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7940.14493997039135617No Hit
GTATCAACGCAGAGTACATGGGAAG7210.1316142552294305No Hit
GGTATCAACGCAGAGTACATGGAAG6730.12285214115035606No Hit
ATACCACTGCTTCCATGTACTCTGC6660.1215743328471577No Hit
GAGTACATGGGAAGCAGTGGTATCA5800.10587554512214935No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5640.10295484042912453No Hit
AAGCAGTGGTATCAACGCAGAGTAC5520.10076431190935593No Hit
AAAAAGTACTCTGCGTTGATACCAC5520.10076431190935593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGAC651.9168874E-716.0693453
AAGACGG551.1257971E-515.5452295
CGCAAGA508.746176E-515.1900582
CGCGGTC400.00526742614.25239910
CGGTCCT400.00527062314.25109712
GGGTATA400.005299472314.2393781
AGAACCG551.9567384E-413.8179825
AACCGCG500.00149651213.30102357
AACATAT500.00149651213.30102357
TAAACTG500.001497543613.29980855
TCTATAC500.001502711713.2937313
AGCGTGT655.4359632E-513.1548596
CTAGGCT655.44063E-513.1536574
CGAACCT604.078693E-412.668816
GCCGGTC751.4732706E-512.6676438
GCGTGTG604.0818416E-412.6676427
GCGCAAG604.110274E-412.6572261
TAATCAT1001.4360376E-712.3498225
TTAAACA853.930765E-612.2939414
GTGTAAT701.0960136E-412.2051821