Basic Statistics
Measure | Value |
---|---|
Filename | SRR4062437_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 547813 |
Sequences flagged as poor quality | 0 |
Sequence length | 25 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTACATGGAAGCAGTGGTATCAACG | 7832 | 1.4296849472356443 | No Hit |
TCCATGTACTCTGCGTTGATACCAC | 6382 | 1.164996084430271 | No Hit |
GAGTACATGGAAGCAGTGGTATCAA | 3200 | 0.5841409386049619 | No Hit |
CATGTACTCTGCGTTGATACCACTG | 2702 | 0.4932340050345647 | No Hit |
GCTTCCATGTACTCTGCGTTGATAC | 2267 | 0.4138273461929527 | No Hit |
CCCATGTACTCTGCGTTGATACCAC | 2067 | 0.3773185375301426 | No Hit |
CATGGAAGCAGTGGTATCAACGCAG | 1734 | 0.3165313711065637 | No Hit |
GTACATGGGAAGCAGTGGTATCAAC | 1731 | 0.3159837389766216 | No Hit |
GCGTTGATACCACTGCTTCCATGTA | 1639 | 0.29918968699172893 | No Hit |
GTATCAACGCAGAGTACATGGAAGC | 1424 | 0.25994271767920807 | No Hit |
GTACTTTTTTTTTTTTTTTTTTTTT | 1243 | 0.22690224583936489 | No Hit |
ACGCAGAGTACATGGAAGCAGTGGT | 1131 | 0.20645731298819123 | No Hit |
ACTCTGCGTTGATACCACTGCTTCC | 1101 | 0.20098099168876973 | No Hit |
CAGTGGTATCAACGCAGAGTACATG | 1090 | 0.19897300721231514 | No Hit |
GTATCAACGCAGAGTACTTTTTTTT | 1074 | 0.19605230251929034 | No Hit |
TATCAACGCAGAGTACATGGAAGCA | 991 | 0.18090114692422413 | No Hit |
TATCAACGCAGAGTACTTTTTTTTT | 870 | 0.15881331768322404 | No Hit |
GAGTACTTTTTTTTTTTTTTTTTTT | 794 | 0.14493997039135617 | No Hit |
GTATCAACGCAGAGTACATGGGAAG | 721 | 0.1316142552294305 | No Hit |
GGTATCAACGCAGAGTACATGGAAG | 673 | 0.12285214115035606 | No Hit |
ATACCACTGCTTCCATGTACTCTGC | 666 | 0.1215743328471577 | No Hit |
GAGTACATGGGAAGCAGTGGTATCA | 580 | 0.10587554512214935 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTT | 564 | 0.10295484042912453 | No Hit |
AAGCAGTGGTATCAACGCAGAGTAC | 552 | 0.10076431190935593 | No Hit |
AAAAAGTACTCTGCGTTGATACCAC | 552 | 0.10076431190935593 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCAAGAC | 65 | 1.9168874E-7 | 16.069345 | 3 |
AAGACGG | 55 | 1.1257971E-5 | 15.545229 | 5 |
CGCAAGA | 50 | 8.746176E-5 | 15.190058 | 2 |
CGCGGTC | 40 | 0.005267426 | 14.252399 | 10 |
CGGTCCT | 40 | 0.005270623 | 14.251097 | 12 |
GGGTATA | 40 | 0.0052994723 | 14.239378 | 1 |
AGAACCG | 55 | 1.9567384E-4 | 13.817982 | 5 |
AACCGCG | 50 | 0.001496512 | 13.3010235 | 7 |
AACATAT | 50 | 0.001496512 | 13.3010235 | 7 |
TAAACTG | 50 | 0.0014975436 | 13.2998085 | 5 |
TCTATAC | 50 | 0.0015027117 | 13.293731 | 3 |
AGCGTGT | 65 | 5.4359632E-5 | 13.154859 | 6 |
CTAGGCT | 65 | 5.44063E-5 | 13.153657 | 4 |
CGAACCT | 60 | 4.078693E-4 | 12.6688 | 16 |
GCCGGTC | 75 | 1.4732706E-5 | 12.667643 | 8 |
GCGTGTG | 60 | 4.0818416E-4 | 12.667642 | 7 |
GCGCAAG | 60 | 4.110274E-4 | 12.657226 | 1 |
TAATCAT | 100 | 1.4360376E-7 | 12.349822 | 5 |
TTAAACA | 85 | 3.930765E-6 | 12.293941 | 4 |
GTGTAAT | 70 | 1.0960136E-4 | 12.205182 | 1 |