##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062437_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547813
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.946295542457005	32.0	32.0	32.0	32.0	32.0
2	30.397491479756777	32.0	32.0	32.0	32.0	32.0
3	30.40256072783961	32.0	32.0	32.0	32.0	32.0
4	30.48650178071714	32.0	32.0	32.0	32.0	32.0
5	30.172845478292775	32.0	32.0	32.0	21.0	32.0
6	33.82879376721618	36.0	36.0	36.0	32.0	36.0
7	33.685834399694784	36.0	36.0	36.0	27.0	36.0
8	33.62586137970439	36.0	36.0	36.0	27.0	36.0
9	33.89468303965039	36.0	36.0	36.0	32.0	36.0
10	33.361376966227525	36.0	36.0	36.0	21.0	36.0
11	33.952646249723905	36.0	36.0	36.0	32.0	36.0
12	33.594642697416816	36.0	36.0	36.0	27.0	36.0
13	33.76077603123694	36.0	36.0	36.0	32.0	36.0
14	33.60600971499399	36.0	36.0	36.0	27.0	36.0
15	33.53552763443	36.0	36.0	36.0	27.0	36.0
16	33.53779483144796	36.0	36.0	36.0	27.0	36.0
17	33.44501682143359	36.0	36.0	36.0	27.0	36.0
18	33.46062798801781	36.0	36.0	36.0	27.0	36.0
19	33.47424212276817	36.0	36.0	36.0	27.0	36.0
20	33.4670444111403	36.0	36.0	36.0	27.0	36.0
21	33.418131734734295	36.0	36.0	36.0	27.0	36.0
22	33.35705432328185	36.0	36.0	36.0	21.0	36.0
23	33.25109115701891	36.0	36.0	36.0	21.0	36.0
24	33.27211110360652	36.0	36.0	36.0	21.0	36.0
25	32.68726919587523	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	13.0
5	62.0
6	88.0
7	27.0
8	85.0
9	89.0
10	75.0
11	18.0
12	32.0
13	22.0
14	89.0
15	118.0
16	183.0
17	273.0
18	440.0
19	669.0
20	1142.0
21	1697.0
22	2537.0
23	3822.0
24	5299.0
25	7332.0
26	9718.0
27	12072.0
28	15847.0
29	20631.0
30	25433.0
31	33393.0
32	45964.0
33	61765.0
34	119515.0
35	179360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.95154344258255	16.49673493753754	11.323933994074144	25.22778762580577
2	15.29200651337359	20.629029784810406	37.43820692374534	26.640756778070667
3	19.417046825740194	25.14179522706404	27.96729445396744	27.47386349322833
4	12.262752400774088	15.994815058239311	35.516303355606674	36.226129185379925
5	13.471564089220008	37.707597213472475	34.362851169118116	14.457987528189395
6	33.84851390220518	34.49244395744876	17.90914486600009	13.74989727434598
7	29.238300515106125	30.798231834289265	21.724144229715414	18.239323420889196
8	28.398201486824483	32.91202376580596	20.542586796896266	18.14718795047329
9	26.993871756361205	14.97308615718561	18.132402220346354	39.90063986610683
10	14.612357835907503	28.167817138094954	33.50987906799935	23.709945957998197
11	36.765721804923515	21.53919257269222	21.148089259279566	20.546996363104704
12	22.627342316325112	24.435394839775693	30.29310483487721	22.644158009021982
13	30.66760239561231	20.149058696887785	24.806868835291603	24.376470072208303
14	23.593207700279084	19.18270891475631	25.74563784270829	31.47844554225631
15	25.64401055563272	26.35746606334842	24.653323489206795	23.345199891812072
16	25.80514289995815	25.62458767436463	25.19330191282545	23.376967512851767
17	23.956795087360184	25.6032970246363	27.094999634476203	23.344908253527304
18	25.277039067854695	24.39241489604752	27.930363262508568	22.400182773589215
19	24.223653571284963	27.985757995591413	25.387761965513583	22.40282646761004
20	23.854951369014334	24.193595549561206	28.664685607348193	23.286767474076267
21	25.091294573501727	26.139125984683716	24.02116498821121	24.74841445360335
22	23.54416541900432	26.322476385819243	25.342992890609718	24.79036530456672
23	22.947157926676574	25.70195016112729	25.0294745038687	26.32141740832744
24	22.85568065506653	27.458144465565145	25.20160111127358	24.48457376809475
25	25.266759516159105	24.80836084090934	24.266616951977802	25.658262690953755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	94.0
2	253.0
3	412.0
4	412.0
5	412.0
6	1136.0
7	1860.0
8	1860.0
9	1860.0
10	1797.5
11	1735.0
12	1735.0
13	1735.0
14	1770.0
15	1805.0
16	1805.0
17	1805.0
18	2850.5
19	3896.0
20	3896.0
21	3896.0
22	6107.0
23	8318.0
24	8318.0
25	8318.0
26	11970.0
27	15622.0
28	15622.0
29	15622.0
30	19570.5
31	23519.0
32	23519.0
33	23519.0
34	28330.0
35	33141.0
36	33141.0
37	33141.0
38	38132.5
39	43124.0
40	43124.0
41	43124.0
42	51788.5
43	60453.0
44	60453.0
45	60453.0
46	80470.0
47	100487.0
48	100487.0
49	100487.0
50	88449.0
51	76411.0
52	76411.0
53	76411.0
54	66709.5
55	57008.0
56	57008.0
57	57008.0
58	51413.5
59	45819.0
60	45819.0
61	45819.0
62	39405.0
63	32991.0
64	32991.0
65	32991.0
66	26784.0
67	20577.0
68	20577.0
69	20577.0
70	15399.5
71	10222.0
72	10222.0
73	10222.0
74	7699.5
75	5177.0
76	5177.0
77	5177.0
78	4091.5
79	3006.0
80	3006.0
81	3006.0
82	2073.5
83	1141.0
84	1141.0
85	1141.0
86	813.5
87	486.0
88	486.0
89	486.0
90	347.0
91	208.0
92	208.0
93	208.0
94	132.0
95	56.0
96	56.0
97	56.0
98	150.5
99	245.0
100	245.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008031937905818227
2	0.0031032487363388604
3	0.0029207046930248095
4	0.013325715161925693
5	0.03249283970990101
6	0.04344548230874404
7	0.06443804728985986
8	0.08616078844423188
9	0.09328000613347986
10	0.1179234519808767
11	0.11737581985093452
12	0.12869355053640566
13	0.1179234519808767
14	0.1212092447605296
15	0.11262967472476923
16	0.11171695450819896
17	0.11920126028407503
18	0.1255903018000668
19	0.12759828627652137
20	0.11518529133116592
21	0.12467758158349657
22	0.14420979421809998
23	0.13325715161925694
24	0.12650302201663707
25	0.12613793393000897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	547813.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.83563056350049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45730683125345	50.16342255647339
2	11.677648955089394	14.208342753641315
3	2.8995813200687564	5.291935739295898
4	1.0375186012241004	2.524723933073166
5	0.5031167025190444	1.530371092238758
6	0.2911974999907647	1.062911011827186
7	0.18857361653210336	0.8030396408559173
8	0.14169324792046187	0.6895998467065361
9	0.09996310065602061	0.5473186435342514
>10	0.5800104259183602	6.814003509969099
>50	0.07072336525918922	3.02548171930383
>100	0.044540670674925474	5.173436255372259
>500	0.00361140573039933	1.4613854333574394
>1k	0.003912356207932607	4.101655958845743
>5k	6.01900955066555E-4	2.6023719055052177
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7832	1.4296849472356443	No Hit
TCCATGTACTCTGCGTTGATACCAC	6382	1.164996084430271	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3200	0.5841409386049619	No Hit
CATGTACTCTGCGTTGATACCACTG	2702	0.4932340050345647	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2267	0.4138273461929527	No Hit
CCCATGTACTCTGCGTTGATACCAC	2067	0.3773185375301426	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1734	0.3165313711065637	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1731	0.3159837389766216	No Hit
GCGTTGATACCACTGCTTCCATGTA	1639	0.29918968699172893	No Hit
GTATCAACGCAGAGTACATGGAAGC	1424	0.25994271767920807	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1243	0.22690224583936489	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1131	0.20645731298819123	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1101	0.20098099168876973	No Hit
CAGTGGTATCAACGCAGAGTACATG	1090	0.19897300721231514	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1074	0.19605230251929034	No Hit
TATCAACGCAGAGTACATGGAAGCA	991	0.18090114692422413	No Hit
TATCAACGCAGAGTACTTTTTTTTT	870	0.15881331768322404	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	794	0.14493997039135617	No Hit
GTATCAACGCAGAGTACATGGGAAG	721	0.1316142552294305	No Hit
GGTATCAACGCAGAGTACATGGAAG	673	0.12285214115035606	No Hit
ATACCACTGCTTCCATGTACTCTGC	666	0.1215743328471577	No Hit
GAGTACATGGGAAGCAGTGGTATCA	580	0.10587554512214935	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	564	0.10295484042912453	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	552	0.10076431190935593	No Hit
AAAAAGTACTCTGCGTTGATACCAC	552	0.10076431190935593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.825440433140506E-4
13	0.0	0.0	0.0	0.0	1.825440433140506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGAC	65	1.9168874E-7	16.069345	3
AAGACGG	55	1.1257971E-5	15.545229	5
CGCAAGA	50	8.746176E-5	15.190058	2
CGCGGTC	40	0.005267426	14.252399	10
CGGTCCT	40	0.005270623	14.251097	12
GGGTATA	40	0.0052994723	14.239378	1
AGAACCG	55	1.9567384E-4	13.817982	5
AACCGCG	50	0.001496512	13.3010235	7
AACATAT	50	0.001496512	13.3010235	7
TAAACTG	50	0.0014975436	13.2998085	5
TCTATAC	50	0.0015027117	13.293731	3
AGCGTGT	65	5.4359632E-5	13.154859	6
CTAGGCT	65	5.44063E-5	13.153657	4
CGAACCT	60	4.078693E-4	12.6688	16
GCCGGTC	75	1.4732706E-5	12.667643	8
GCGTGTG	60	4.0818416E-4	12.667642	7
GCGCAAG	60	4.110274E-4	12.657226	1
TAATCAT	100	1.4360376E-7	12.349822	5
TTAAACA	85	3.930765E-6	12.293941	4
GTGTAAT	70	1.0960136E-4	12.205182	1
>>END_MODULE