FastQCFastQC Report
Thu 2 Feb 2017
SRR4062437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547813
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA57141.043056663496485No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49120.8966563407586164No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA21110.3853504754359608No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20030.36563571875804335No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG17520.3198171638862166No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC13420.24497410612745588No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA13130.23968032887134844No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10990.2006159036021416No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10830.19769519890911683No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10260.18729018844021592No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8130.14840830721432316No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG7960.14530505847798428No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA7730.1411065454817611No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC6920.12632047797332302No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA6690.12212196497709984No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6120.11171695450819896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTC300.005742422329.33321442
CTATACT551.5921124E-423.99994
ACGACGG1153.5106495E-1022.9564276
GTCTAGG500.002575982622.0039271
CTACGAC1205.966285E-1021.999914
GACGGTA1205.966285E-1021.999918
CGAATGT703.211318E-521.9999133
AACGAAT703.211318E-521.9999131
CCGTCGT803.609699E-621.9999089
GTCGTAG803.609699E-621.99990811
CGCCGGT1451.2732926E-1121.2412917
CGACGGT1259.931682E-1021.1199117
ACGGTAT1259.931682E-1021.1199119
TGGTATA2300.021.0433944
TCCAACG956.8524787E-720.84201828
GCATTCG856.1036026E-620.70579722
CGTCGTA856.1036026E-620.70579710
ATAATAC755.4658132E-520.5332473
ACTATAC654.927568E-420.3076083
CGTGCGC654.927568E-420.30760810
Produced by FastQC (version 0.11.5)