##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547813
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19987842566715	32.0	32.0	32.0	32.0	32.0
2	31.37261437753394	32.0	32.0	32.0	32.0	32.0
3	31.49270827818982	32.0	32.0	32.0	32.0	32.0
4	31.611069835874652	32.0	32.0	32.0	32.0	32.0
5	31.537878801707883	32.0	32.0	32.0	32.0	32.0
6	35.129177292251185	36.0	36.0	36.0	36.0	36.0
7	35.149804769145675	36.0	36.0	36.0	36.0	36.0
8	35.095912291238065	36.0	36.0	36.0	36.0	36.0
9	35.211992048381475	36.0	36.0	36.0	36.0	36.0
10	35.10057629154474	36.0	36.0	36.0	36.0	36.0
11	35.22588730095854	36.0	36.0	36.0	36.0	36.0
12	35.14592570822525	36.0	36.0	36.0	36.0	36.0
13	35.17853172524201	36.0	36.0	36.0	36.0	36.0
14	35.14686763548875	36.0	36.0	36.0	36.0	36.0
15	35.10866116722312	36.0	36.0	36.0	36.0	36.0
16	35.10940777236028	36.0	36.0	36.0	36.0	36.0
17	35.092673959909675	36.0	36.0	36.0	36.0	36.0
18	35.103058890533816	36.0	36.0	36.0	36.0	36.0
19	35.0820498965888	36.0	36.0	36.0	36.0	36.0
20	35.067566852192265	36.0	36.0	36.0	36.0	36.0
21	35.045144967351995	36.0	36.0	36.0	36.0	36.0
22	35.01775970997402	36.0	36.0	36.0	36.0	36.0
23	34.95921966072364	36.0	36.0	36.0	32.0	36.0
24	34.95698714707391	36.0	36.0	36.0	32.0	36.0
25	34.94167535272073	36.0	36.0	36.0	32.0	36.0
26	34.89035126950255	36.0	36.0	36.0	32.0	36.0
27	34.878233995907365	36.0	36.0	36.0	32.0	36.0
28	34.84222170704237	36.0	36.0	36.0	32.0	36.0
29	34.82244488538972	36.0	36.0	36.0	32.0	36.0
30	34.79591028325359	36.0	36.0	36.0	32.0	36.0
31	34.76783500939189	36.0	36.0	36.0	32.0	36.0
32	34.72923242055227	36.0	36.0	36.0	32.0	36.0
33	34.68138397591879	36.0	36.0	36.0	32.0	36.0
34	34.66082586576076	36.0	36.0	36.0	32.0	36.0
35	34.59255256812087	36.0	36.0	36.0	32.0	36.0
36	34.55791483590203	36.0	36.0	36.0	32.0	36.0
37	34.51154682345983	36.0	36.0	36.0	32.0	36.0
38	34.422949072037355	36.0	36.0	36.0	32.0	36.0
39	34.337544015932444	36.0	36.0	36.0	32.0	36.0
40	34.21888856233788	36.0	36.0	36.0	32.0	36.0
41	34.24031010582078	36.0	36.0	36.0	32.0	36.0
42	34.10500481003554	36.0	36.0	36.0	32.0	36.0
43	34.09397184805764	36.0	36.0	36.0	32.0	36.0
44	34.12168385927315	36.0	36.0	36.0	32.0	36.0
45	33.910000310324875	36.0	36.0	36.0	32.0	36.0
46	34.01943911517251	36.0	36.0	36.0	32.0	36.0
47	33.94118065836334	36.0	36.0	36.0	32.0	36.0
48	33.901084859249416	36.0	36.0	36.0	32.0	36.0
49	33.96053032695463	36.0	36.0	36.0	32.0	36.0
50	33.30872761325489	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	12.0
22	31.0
23	136.0
24	419.0
25	951.0
26	1921.0
27	3575.0
28	6240.0
29	9549.0
30	13948.0
31	21022.0
32	32349.0
33	49446.0
34	97025.0
35	311185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.60497041376905	16.97999528957261	11.74430047414651	25.670733822511828
2	14.818560532762795	20.99597287992026	37.10682638840715	27.078640198909792
3	18.890673773753676	25.491867618334823	28.23825779010971	27.37920081780179
4	12.124414945273196	16.398326433155898	35.66552028798201	35.81173833358889
5	13.21691891211052	37.83535622557332	34.37632914881538	14.571395713500776
6	34.09716124721848	34.70521767543916	17.63911275969267	13.5585083176497
7	29.231690376095493	30.67451849444975	21.71762992115923	18.37616120829553
8	28.481434358074743	33.17172100698596	20.375018482584387	17.971826152354907
9	27.044995281236478	14.640214817830172	17.883292291347594	40.431497609585755
10	14.520465925416154	28.286294775772024	33.585548353178915	23.60769094563291
11	37.134022011160745	21.302524766663076	21.073797080390573	20.48965614178561
12	22.645501293324546	24.36451854921296	30.38171784897401	22.60826230848848
13	30.82858566700681	20.099559521223483	24.769218693240212	24.3026361185295
14	23.70608218497918	19.068733308629042	25.632286930029043	31.592897576362738
15	25.767917154211382	26.495902798947817	24.489196130796458	23.246983916044346
16	25.91778581377222	25.478219757471983	25.083194447740382	23.52079998101542
17	23.878951393997585	25.69325664049594	27.097750509754242	23.330041455752237
18	25.48716441559437	24.202601982793396	27.87228488553576	22.437948716076473
19	24.14948166618901	28.03146329130561	25.36960605170012	22.44944899080526
20	23.973509208434265	24.110052152833177	28.58566700680707	23.330771631925494
21	25.07187671705491	25.99664484048389	24.072630623953795	24.85884781850741
22	23.49652162325465	26.234134640835467	25.334922683470456	24.934421052439426
23	22.93994483519011	25.424551808737654	25.137227484561336	26.498275871510902
24	22.9255238557683	27.332137061369483	25.221380288529115	24.520958794333104
25	25.291659745205024	24.64983488891282	24.447211000834226	25.611294365047925
26	23.157902422907085	25.198379739071548	25.921619238681814	25.722098599339553
27	25.577888805121457	26.066741753116485	25.3004218592841	23.054947582477965
28	24.352470642354234	24.080845105902927	25.910484052039656	25.656200199703182
29	23.119385629767823	26.384185844439617	27.050106514449272	23.446322011343288
30	25.50505373183915	25.020034208753717	26.34858975599338	23.126322303413758
31	23.15899768716697	25.683764350243603	25.71899535060322	25.438242611986205
32	23.43299629618136	26.03461400149321	25.830712305111415	24.701677397214013
33	23.15078320521784	25.352081823541976	25.953199358175144	25.543935613065045
34	23.041074235186095	26.514157203279222	26.888372492073024	23.55639606946166
35	25.129378090698832	25.562372561439762	25.98350166936528	23.32474767849613
36	24.217205506258523	26.40335296898759	25.750758014139862	23.628683510614024
37	25.415607150615266	25.971271218463233	25.847871445182935	22.765250185738566
38	24.177045816729432	25.96469963290393	24.737821117790197	25.120433432576444
39	23.789687356817016	25.90683317117338	26.199268728562487	24.104210743447123
40	25.302794931847185	25.850244517746017	24.61588169685641	24.23107885355039
41	22.7267333925993	25.902999746263777	26.44789371555622	24.9223731455807
42	26.217705676937204	26.025304255284194	25.170267956401183	22.586722111377423
43	25.043947478427857	24.19949873405706	24.99100970586678	25.7655440816483
44	24.362510564736507	25.214261070839868	27.05740827618184	23.36582008824179
45	25.59340504880315	25.16935523618461	25.576063364688316	23.661176350323924
46	23.920797645907722	25.202989346710186	27.023139325169947	23.853073682212145
47	25.218824671922718	24.48664051419006	27.02016929134577	23.27436552254145
48	23.995597037675264	28.177315981913537	24.563491556425276	23.263595423985922
49	23.110440971645435	25.329081274084402	28.119632064226295	23.440845690043865
50	23.494513638778198	27.55684877868908	24.016041970526437	24.932595612006285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	12.5
2	13.0
3	60.0
4	107.0
5	103.5
6	100.0
7	110.0
8	120.0
9	153.5
10	187.0
11	278.0
12	369.0
13	598.5
14	828.0
15	1127.0
16	1426.0
17	1605.5
18	1785.0
19	2005.5
20	2226.0
21	2561.0
22	2896.0
23	3332.5
24	3769.0
25	4050.0
26	4331.0
27	5078.0
28	5825.0
29	6678.0
30	7531.0
31	8502.0
32	9473.0
33	10789.5
34	12106.0
35	13785.5
36	15465.0
37	17469.0
38	19473.0
39	21176.0
40	22879.0
41	25326.0
42	27773.0
43	29420.5
44	31068.0
45	38605.0
46	46142.0
47	52392.5
48	58643.0
49	55038.5
50	51434.0
51	45166.0
52	38898.0
53	36346.5
54	33795.0
55	33464.5
56	33134.0
57	31559.5
58	29985.0
59	27442.5
60	24900.0
61	21958.5
62	19017.0
63	16349.0
64	13681.0
65	11537.5
66	9394.0
67	7928.0
68	6462.0
69	5788.5
70	5115.0
71	3905.0
72	2695.0
73	2364.0
74	2033.0
75	1464.5
76	896.0
77	800.5
78	705.0
79	592.5
80	480.0
81	376.0
82	272.0
83	221.5
84	171.0
85	147.5
86	124.0
87	89.0
88	54.0
89	33.5
90	13.0
91	9.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016794051984892654
2	0.004928689169479366
3	5.476321299421518E-4
4	1.825440433140506E-4
5	0.0
6	3.650880866281012E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.825440433140506E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	547813.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.1784221733135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9704900458557	51.78766225870463
2	12.003231256033585	15.166904234752037
3	2.998824954655822	5.683830870273397
4	1.1254844967514592	2.844253387411319
5	0.5088057897011222	1.6072773492981836
6	0.2916936322421115	1.105724606583562
7	0.19166727111349935	0.8476465039850917
8	0.12623255974064465	0.6380139161049971
9	0.09604297912994442	0.5461059494028929
>10	0.577487799259965	7.015287722330201
>50	0.06312776366891945	2.717906837720293
>100	0.04169906830867941	4.967277980019787
>500	0.002316614906037627	0.9822590503011119
>1k	0.0026061917692923302	3.0444725006632156
>5k	2.895768632547034E-4	1.0453768324493489
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5714	1.043056663496485	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4912	0.8966563407586164	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2111	0.3853504754359608	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2003	0.36563571875804335	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1752	0.3198171638862166	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1342	0.24497410612745588	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1313	0.23968032887134844	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1099	0.2006159036021416	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1083	0.19769519890911683	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1026	0.18729018844021592	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	813	0.14840830721432316	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	796	0.14530505847798428	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	773	0.1411065454817611	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	692	0.12632047797332302	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	669	0.12212196497709984	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	612	0.11171695450819896	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	1.825440433140506E-4
16	0.0	0.0	0.0	0.0	1.825440433140506E-4
17	0.0	0.0	0.0	0.0	1.825440433140506E-4
18	0.0	0.0	0.0	0.0	1.825440433140506E-4
19	0.0	0.0	0.0	1.825440433140506E-4	1.825440433140506E-4
20	0.0	0.0	0.0	1.825440433140506E-4	3.650880866281012E-4
21	0.0	0.0	0.0	1.825440433140506E-4	3.650880866281012E-4
22	0.0	0.0	0.0	7.301761732562024E-4	3.650880866281012E-4
23	0.0	0.0	0.0	0.0010952642598843036	3.650880866281012E-4
24	0.0	0.0	0.0	0.0023730725630826577	3.650880866281012E-4
25	0.0	0.0	0.0	0.0034683368229669613	3.650880866281012E-4
26	0.0	0.0	0.0	0.0038334249095950627	3.650880866281012E-4
27	0.0	0.0	0.0	0.0045636010828512645	3.650880866281012E-4
28	0.0	0.0	0.0	0.006571585559305822	3.650880866281012E-4
29	0.0	0.0	0.0	0.010405010468900884	3.650880866281012E-4
30	0.0	0.0	0.0	0.01569878772500835	3.650880866281012E-4
31	0.0	0.0	0.0	0.02792923862704974	3.650880866281012E-4
32	0.0	0.0	0.0	0.044540746568628345	3.650880866281012E-4
33	0.0	0.0	0.0	0.06370787111660366	3.650880866281012E-4
34	0.0	0.0	0.0	0.08981166931051289	7.301761732562024E-4
35	0.0	0.0	0.0	0.11792345198087668	7.301761732562024E-4
36	0.0	0.0	0.0	0.1537020844704306	7.301761732562024E-4
37	0.0	0.0	0.0	0.21010819385447224	7.301761732562024E-4
38	1.825440433140506E-4	0.0	0.0	0.2816654588335801	7.301761732562024E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGTC	30	0.0057424223	29.333214	42
CTATACT	55	1.5921124E-4	23.9999	4
ACGACGG	115	3.5106495E-10	22.956427	6
GTCTAGG	50	0.0025759826	22.003927	1
CTACGAC	120	5.966285E-10	21.99991	4
GACGGTA	120	5.966285E-10	21.99991	8
CGAATGT	70	3.211318E-5	21.99991	33
AACGAAT	70	3.211318E-5	21.99991	31
CCGTCGT	80	3.609699E-6	21.999908	9
GTCGTAG	80	3.609699E-6	21.999908	11
CGCCGGT	145	1.2732926E-11	21.241291	7
CGACGGT	125	9.931682E-10	21.119911	7
ACGGTAT	125	9.931682E-10	21.119911	9
TGGTATA	230	0.0	21.04339	44
TCCAACG	95	6.8524787E-7	20.842018	28
GCATTCG	85	6.1036026E-6	20.705797	22
CGTCGTA	85	6.1036026E-6	20.705797	10
ATAATAC	75	5.4658132E-5	20.533247	3
ACTATAC	65	4.927568E-4	20.307608	3
CGTGCGC	65	4.927568E-4	20.307608	10
>>END_MODULE