FastQCFastQC Report
Thu 2 Feb 2017
SRR4062436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118357
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG70580.3331827449292069No Hit
TCCATGTACTCTGCGTTGATACCAC59070.2788481828133785No Hit
GTATCAACGCAGAGTACTTTTTTTT37500.17702398604201275No Hit
GAGTACATGGAAGCAGTGGTATCAA28400.13406616542915098No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27870.13156422642642387No Hit
TATCAACGCAGAGTACTTTTTTTTT27310.12892066823486314No Hit
GAATAGGACCGCGGTTCTATTTTGT25350.1196682145644006No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25170.11881849943139895No Hit
GAATAACGCCGCCGCATCGCCAGTC23980.11320093827433242No Hit
GATTAAGAGGGACGGCCGGGGGCAT23480.11084061846043892No Hit
CATGTACTCTGCGTTGATACCACTG23360.11027414170510447No Hit
GCGCAAGACGGACCAGAGCGAAAGC22710.10720572594704293No Hit
GTATCTGATCGTCTTCGAACCTCCG22630.10682807477681996No Hit
GTCCTATTCCATTATTCCTAGCTGC22520.1063088044177634No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22470.10607277243637404No Hit
CTATTGGAGCTGGAATTACCGCGGC22280.1051758509070945No Hit
GAACTACGACGGTATCTGATCGTCT21920.1034764206410912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT4200.012.8949119
CGTCTTA1800.012.66748515
CGCATCG4100.012.51330113
AATCGTA1001.4417128E-712.3510913
CCGTCGT3650.012.2348259
GCGTAAC1800.012.14082111
ATCGCCA4400.012.09226316
CGTCGTA3700.012.06977510
CGGACAT2450.012.0183455
CGCGTAA1900.012.00219510
ACGCGCG951.0392414E-612.001060513
CAAGACG5150.011.9876664
CTATACT1353.7289283E-1011.9603444
CCGCATC4550.011.90327612
AGGCCCG5200.011.87717210
CGGTCCA4800.011.877171510
CGCCAGT4500.011.82466518
GTAATAC1052.7392707E-711.7559963
GCGTTAT1302.6411726E-911.6853291
CATCGCC4400.011.65984615
Produced by FastQC (version 0.11.5)