##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062436_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2118357
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.123504678389903	32.0	32.0	32.0	32.0	32.0
2	30.66854925775023	32.0	32.0	32.0	32.0	32.0
3	30.66825705015727	32.0	32.0	32.0	32.0	32.0
4	30.761573710191435	32.0	32.0	32.0	32.0	32.0
5	30.49154604252258	32.0	32.0	32.0	32.0	32.0
6	34.14758607732313	36.0	36.0	36.0	32.0	36.0
7	34.02158937327372	36.0	36.0	36.0	32.0	36.0
8	34.01703253984102	36.0	36.0	36.0	32.0	36.0
9	34.2268706360637	36.0	36.0	36.0	32.0	36.0
10	33.81713705480238	36.0	36.0	36.0	32.0	36.0
11	34.21105460505477	36.0	36.0	36.0	32.0	36.0
12	33.93072225314241	36.0	36.0	36.0	32.0	36.0
13	34.08454099096611	36.0	36.0	36.0	32.0	36.0
14	33.96950561213242	36.0	36.0	36.0	32.0	36.0
15	33.88580914359572	36.0	36.0	36.0	32.0	36.0
16	33.8875317049959	36.0	36.0	36.0	32.0	36.0
17	33.80006769397226	36.0	36.0	36.0	32.0	36.0
18	33.81741746079627	36.0	36.0	36.0	32.0	36.0
19	33.807144404838276	36.0	36.0	36.0	32.0	36.0
20	33.80482232220537	36.0	36.0	36.0	32.0	36.0
21	33.76964505982703	36.0	36.0	36.0	32.0	36.0
22	33.721103666662415	36.0	36.0	36.0	27.0	36.0
23	33.670864259423695	36.0	36.0	36.0	27.0	36.0
24	33.63383839456711	36.0	36.0	36.0	27.0	36.0
25	33.15268247986529	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	102.0
5	202.0
6	353.0
7	101.0
8	324.0
9	370.0
10	238.0
11	83.0
12	158.0
13	105.0
14	334.0
15	450.0
16	714.0
17	973.0
18	1311.0
19	1961.0
20	3229.0
21	4738.0
22	7174.0
23	10813.0
24	15144.0
25	21569.0
26	29356.0
27	37900.0
28	50344.0
29	66350.0
30	84696.0
31	114121.0
32	161616.0
33	228817.0
34	482693.0
35	792006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58786733151353	17.30601997373219	11.235200752343284	25.87091194241099
2	16.52977771399383	19.88743602066222	37.3620921302464	26.220694135097546
3	18.887864457861188	23.862539295427496	28.413043036961188	28.836553209750125
4	12.266110302973647	15.303259288426702	36.08545451712645	36.3451758914732
5	14.345558984975614	36.71218308821585	33.73148396504762	15.210773961760918
6	34.27430247912963	35.40543016856873	16.950101919563835	13.37016543273781
7	30.178358096499586	30.568547432804532	20.849998653705075	18.40309581699081
8	28.22202125980755	33.04696189242337	19.5209786806597	19.210038167109385
9	27.28503851731377	14.294098107045198	18.591603069666654	39.82926030597438
10	15.671422413956074	26.64393713112554	31.726039465077676	25.958600989840708
11	37.472085963652795	21.00477770147613	22.202913126556393	19.320223208314687
12	24.4029496943906	23.76734075152828	28.95780836629075	22.87190118779037
13	29.557312159559512	19.342431439273096	25.41462550069122	25.685630900476177
14	23.467195270545552	19.85743197958939	24.941841284605733	31.733531465259325
15	25.225605857174653	27.365382007014283	22.45396789839549	24.955044237415578
16	25.841238515569582	25.697855924597956	24.139078277726504	24.321827282105957
17	23.924528658597108	25.89326817187123	25.626556761840842	24.55564640769082
18	25.05336476923164	24.548171984866904	26.769403694789446	23.62905955111201
19	25.429621324419166	25.48596510467086	25.20150464363504	23.882908927274926
20	25.25314158346772	24.355566562624656	25.61224721289037	24.779044641017258
21	26.551869522431147	24.48581397701158	24.045765084790947	24.91655141576632
22	25.364774828397575	24.95124595252762	24.80908505967968	24.874894159395122
23	23.871053135392316	24.539044976352493	25.398911340167196	26.19099054808799
24	24.39400120157481	25.59406340741469	25.1096992423272	24.9022361486833
25	24.831277976564678	24.6700478821711	25.09890764365833	25.399766497605892
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	225.0
1	225.0
2	641.5
3	1058.0
4	1058.0
5	1058.0
6	2676.0
7	4294.0
8	4294.0
9	4294.0
10	4535.5
11	4777.0
12	4777.0
13	4777.0
14	5478.0
15	6179.0
16	6179.0
17	6179.0
18	10117.5
19	14056.0
20	14056.0
21	14056.0
22	22836.5
23	31617.0
24	31617.0
25	31617.0
26	47562.0
27	63507.0
28	63507.0
29	63507.0
30	79085.0
31	94663.0
32	94663.0
33	94663.0
34	115516.0
35	136369.0
36	136369.0
37	136369.0
38	153095.0
39	169821.0
40	169821.0
41	169821.0
42	195607.0
43	221393.0
44	221393.0
45	221393.0
46	258505.5
47	295618.0
48	295618.0
49	295618.0
50	288014.5
51	280411.0
52	280411.0
53	280411.0
54	256689.5
55	232968.0
56	232968.0
57	232968.0
58	213703.5
59	194439.0
60	194439.0
61	194439.0
62	173470.0
63	152501.0
64	152501.0
65	152501.0
66	126389.5
67	100278.0
68	100278.0
69	100278.0
70	75783.0
71	51288.0
72	51288.0
73	51288.0
74	40463.0
75	29638.0
76	29638.0
77	29638.0
78	24396.5
79	19155.0
80	19155.0
81	19155.0
82	13514.0
83	7873.0
84	7873.0
85	7873.0
86	5757.5
87	3642.0
88	3642.0
89	3642.0
90	2520.0
91	1398.0
92	1398.0
93	1398.0
94	845.5
95	293.0
96	293.0
97	293.0
98	594.5
99	896.0
100	896.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008261119348627262
2	0.004484607646397657
3	0.005098290798009967
4	0.015011634016362681
5	0.03460228847167876
6	0.04725360267414794
7	0.06778838505502141
8	0.08851199302100637
9	0.09611222282174345
10	0.11617494123983822
11	0.11735510114678498
12	0.12972317697158695
13	0.119526595375567
14	0.12250059834107282
15	0.11272887431155372
16	0.11046296729021596
17	0.12212294717084986
18	0.1288262554423074
19	0.1309033368785337
20	0.12014027852717932
21	0.1266547612135254
22	0.14860573548273495
23	0.13746502596115764
24	0.13217790957803618
25	0.12967597057530908
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2118357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.4507807116506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82038387709612	37.400887509602086
2	12.20026993205489	11.57824666337762
3	3.7734174106647282	5.371548062609294
4	1.5983075983762876	3.033637734412726
5	0.8413307405528915	1.996090023797293
6	0.5126946429244754	1.4596656644068375
7	0.3533044740938734	1.17352011872714
8	0.27399436811653116	1.0400997342179834
9	0.20142258819892608	0.860189315670031
>10	1.1884349430114722	10.49151880849297
>50	0.11136737811895465	3.6882013394194937
>100	0.10153558343030389	10.43278388148018
>500	0.01625611664314045	5.374902682582251
>1k	0.007080885163576517	5.485165347779308
>5k	1.9946155390356386E-4	0.6135431134248309
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7058	0.3331827449292069	No Hit
TCCATGTACTCTGCGTTGATACCAC	5907	0.2788481828133785	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3750	0.17702398604201275	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2840	0.13406616542915098	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2787	0.13156422642642387	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2731	0.12892066823486314	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2535	0.1196682145644006	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2517	0.11881849943139895	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2398	0.11320093827433242	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2348	0.11084061846043892	No Hit
CATGTACTCTGCGTTGATACCACTG	2336	0.11027414170510447	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2271	0.10720572594704293	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2263	0.10682807477681996	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2252	0.1063088044177634	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2247	0.10607277243637404	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2228	0.1051758509070945	No Hit
GAACTACGACGGTATCTGATCGTCT	2192	0.1034764206410912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.7206396277870065E-5	0.0
10	0.0	0.0	0.0	4.7206396277870065E-5	0.0
11	0.0	0.0	0.0	9.441279255574013E-5	0.0
12	0.0	0.0	0.0	1.8882558511148026E-4	0.0
13	0.0	0.0	0.0	1.8882558511148026E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAT	420	0.0	12.894911	9
CGTCTTA	180	0.0	12.667485	15
CGCATCG	410	0.0	12.513301	13
AATCGTA	100	1.4417128E-7	12.35109	13
CCGTCGT	365	0.0	12.234825	9
GCGTAAC	180	0.0	12.140821	11
ATCGCCA	440	0.0	12.092263	16
CGTCGTA	370	0.0	12.069775	10
CGGACAT	245	0.0	12.018345	5
CGCGTAA	190	0.0	12.002195	10
ACGCGCG	95	1.0392414E-6	12.0010605	13
CAAGACG	515	0.0	11.987666	4
CTATACT	135	3.7289283E-10	11.960344	4
CCGCATC	455	0.0	11.903276	12
AGGCCCG	520	0.0	11.877172	10
CGGTCCA	480	0.0	11.8771715	10
CGCCAGT	450	0.0	11.824665	18
GTAATAC	105	2.7392707E-7	11.755996	3
GCGTTAT	130	2.6411726E-9	11.685329	1
CATCGCC	440	0.0	11.659846	15
>>END_MODULE