FastQCFastQC Report
Thu 2 Feb 2017
SRR4062436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118357
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29880.14105271207827577No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA26590.1255218077028565No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26250.12391679022940892No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25860.12207574077457198No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25350.1196682145644006No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24140.11395624061477834No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22240.10498702532198304No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA22150.1045621677554822No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG21630.10210743514903295No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21620.10206022875275508No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA21510.1015409583936985No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC21310.10059683046814111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC5450.019.7834571
GATTTCG3600.019.5554641
ATTTCGT3900.019.17939442
CTAACCG600.00741410218.33324437
GTATTAC1951.8189894E-1218.0546041
TAGGACG6150.017.8860934
ATAACGC8300.017.4938933
CGCAATA8700.017.4481936
ATACGAA8550.017.23968140
ACTGTTC6800.017.1469768
TAACGCC8400.017.0237274
GTAGGAC10250.016.9560173
AATAACG8700.016.9428462
TAGCGGC8750.016.84563330
TGTAGGA10600.016.8116362
AATCCCG2900.016.68957519
CGGTCCA9100.016.68123810
CATCGTT8750.016.59420628
GGCGTTA7050.016.53892942
ATCGTTT8650.016.53171229