FastQCFastQC Report
Thu 2 Feb 2017
SRR4062435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719506
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37070.5152146055766039No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32100.4461394345564874No Hit
GTATTAGAGGCACTGCCTGCCCAGT30620.4255697659227303No Hit
TATCAACGCAGAGTACTTTTTTTTT25540.3549657681798345No Hit
CCATAGGGTCTTCTCGTCTTATTAT24690.34315210714017674No Hit
CTCTAATACTTGTAATGCTAGAGGT23710.32953165088268893No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22370.3109077617142873No Hit
GTACATGGAAGCAGTGGTATCAACG21890.30423651783306876No Hit
CCATTGGGATGTCCTGATCCAACAT20150.2800532587636517No Hit
TCCATGTACTCTGCGTTGATACCAC19460.2704633456844001No Hit
GTCAGGATACCGCGGCCGTTAAACT17450.24252751193179767No Hit
CTTCTACACCATTGGGATGTCCTGA16970.23585626805057913No Hit
GATATATTTTGATCAACGGACCAAG16430.22835111868420832No Hit
CCTCTAGCATTACAAGTATTAGAGG16060.22320870152576908No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15740.2187612056049567No Hit
GTCCTAAAGTGTGTATTTCTCATTT15450.21473066242672056No Hit
ATCTATAACTTTATAGATGCAACAC14510.20166614315933434No Hit
ATCGTAAATAGATAGAAACCGACCT14490.20138817466428355No Hit
GCCTAAAGGAAAGATCCAAAAAGAT14310.19888645820882658No Hit
GTATCCTGACCGTGCAAAGGTAGCA13990.19443896228801427No Hit
CTGTTAGTATGAGTAACAAGAATTC13850.19249318282265887No Hit
GTACATGGGAGAAATCGTAAATAGA13820.19207623008008273No Hit
GTTATATAATTTAAGCTCCATAGGG13770.1913813088424558No Hit
GGTCAGGATACCGCGGCCGTTAAAC13710.1905474033573035No Hit
GTTATAGATTAACCCAATTTTAAGT13630.1894355293771004No Hit
ATCCTGACCGTGCAAAGGTAGCATA13190.18332022248598345No Hit
GTACATGGGGTGGTATCAACGCAAA13120.18234733275330575No Hit
TGCTAGAGGTGATGTTTTTGGTAAA13100.18206936425825496No Hit
ACCTATAACTTCTCTGTTAACCCAA13070.18165241151567882No Hit
GTTCATGCTAGTCCCTAATTAAGGA13060.18151342726815342No Hit
ATAAATAATCCACCTATAACTTCTC12690.17637101010971418No Hit
CCTATAACTTCTCTGTTAACCCAAC12320.1712285929512749No Hit
GTATCAACGCAGAGTACATGGGAGA12210.16969976622849567No Hit
CTATAGAACTAGTACCGCAAGGGAA12190.16942179773344487No Hit
CACTATAAATAATCCACCTATAACT12150.16886586074334334No Hit
TCCCAATGGTGTAGAAGCTATTAAT11890.16525227030768333No Hit
GTCCTACAGTGGACATTTCTAAATT11850.1646963333175818No Hit
CTTTAGGACGTGAAATATGGCGAGG11850.1646963333175818No Hit
GATAGAAACCGACCTGGATTGCTCC11730.16302852234727716No Hit
GATTAAAGATAAGAGACAGTTGGAC11670.16219461686212486No Hit
GGTATCAACGCAGAGTACTTTTTTT11640.1617776641195487No Hit
CTGTAGGACGTGGAATATGGCAAGA11390.15830305793141405No Hit
GATTAAACCTTGTACCTTTTGCATA11290.1569132154561602No Hit
GTGTAGAAGCTATTAATGGTTCGTT11000.15288267227792401No Hit
GACTATAGGCAATAATCACACTATA10980.15260470378287325No Hit
CTGTTAACCCAACACCGGAATGCCT10510.14607244414918014No Hit
AAATATATCTGGGTCAATAAGATAT10210.14190291672341857No Hit
GACCTGGATTGCTCCGGTCTGAACT9950.13828932628775853No Hit
ACCTTTGCACGGTCAGGATACCGCG9830.13662151531745392No Hit
CCATTAATAGCTTCTACACCATTGG9580.13314690912931929No Hit
AGCATGAACGGCTAAACGAGGGTCC9280.12897738170355771No Hit
GGATACCGCGGCCGTTAAACTTTAG9190.12772652347582925No Hit
CTTTATTGGTGGCTGCTTTTAGGCC9160.1273095707332531No Hit
CCTATAGTCTGATTAACTAACAATG9140.1270316022382023No Hit
CTTAAATAGGATTGCGCTGTTATCC9140.1270316022382023No Hit
GTCCTGATCCAACATCGAGGTCGTA8890.12355699605006767No Hit
GTTAGTATGAGTAACAAGAATTCCA8850.12300105905996615No Hit
GGATTGCTCCGGTCTGAACTCAGAT8830.12272309056491537No Hit
GAGTACATGGAAGCAGTGGTATCAA8790.12216715357481382No Hit
AGGGATAACAGCGCAATCCTATTTA8730.12133324808966152No Hit
ATTTAAGAGTTCATATCGACAATTA8610.11966543711935688No Hit
GTTATACGCGTATGCCTGGAGAATT8600.11952645287183152No Hit
GTTTAAAATTGAACTTAAATTCATT8560.11897051588172997No Hit
TACTAACAGTGTTGCATCTATAAAG8480.11785864190152688No Hit
GTACATGGGTGGTATCAACGCAAAA8480.11785864190152688No Hit
CTATTAAAGGTTTTTTCCGTTCCAG8380.11646879942627303No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8230.11438403571339224No Hit
GCTATCACCAAGCTCGTTAGGCTTT8140.11313317748566377No Hit
CATGTACTCTGCGTTGATACCACTG8080.11229927200051146No Hit
CAGTTGGACCCTCGTTTAGCCGTTC8010.11132638226783376No Hit
GGGCAGGCAGTGCCTCTAATACTTG7860.10924161855495299No Hit
ATTATAACCTAGACTTACAAGTCAA7700.1070178705945468No Hit
CCAATAAAGAAAGCGTTCAAGCTCA7700.1070178705945468No Hit
GTTTAGATTATAGCCAAAAGAGGGA7620.10590599661434374No Hit
ATTTCAGCCTCTTCACTGAAAGGTC7570.1052110753767168No Hit
GATAAAAGGAACTCGGCAAACAAGA7480.10396021714898834No Hit
CCTTTAGGCATTCCGGTGTTGGGTT7480.10396021714898834No Hit
TTGTAGAACAGTGTATATCAATGAG7440.1034042801588868No Hit
AATCTAAACTTACTTTTTGATTTTG7340.10201443768363294No Hit
ACGTAGGACTTTAATCGTTGAACAA7250.10076357945590446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAATG350.002167134316.28744515
TTGCTAA702.4951078E-816.2863110
TCTGTAC350.002175208916.278383
GACTTAA551.1262329E-515.5460247
GTTCTAA551.1329377E-515.5362931
CCGGCCA400.005274164514.2505239
ACTGTGC400.005274164514.2505238
TAGCCTA400.00527660114.2495315
TAGACTG602.5652682E-514.249535
AAATGTC1750.014.1148027
TCCAACG1101.8371793E-1013.82349918
CCAACGA551.9514638E-413.82349919
CATTCCG1257.2759576E-1213.6805019
TAGAAAT1900.013.4976774
ATGTCCA1850.013.3518419
CGTCTGC500.001497765413.300487510
CTGTGCA500.001497765413.30048759
CTGTACT500.00150012413.297714
ACTATAT500.001503273813.294013
CTCTAGG500.001504850913.2921611
Produced by FastQC (version 0.11.5)