##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062435_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719506
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.951935077678296	32.0	32.0	32.0	32.0	32.0
2	30.749164009751134	32.0	32.0	32.0	32.0	32.0
3	30.783343015902577	32.0	32.0	32.0	32.0	32.0
4	30.773993545571546	32.0	32.0	32.0	32.0	32.0
5	30.723599247261316	32.0	32.0	32.0	32.0	32.0
6	34.271769797611135	36.0	36.0	36.0	32.0	36.0
7	34.05599119395808	36.0	36.0	36.0	32.0	36.0
8	34.02290043446476	36.0	36.0	36.0	32.0	36.0
9	34.134260172952	36.0	36.0	36.0	32.0	36.0
10	33.92440090840104	36.0	36.0	36.0	32.0	36.0
11	34.264549565952194	36.0	36.0	36.0	32.0	36.0
12	34.053118389561725	36.0	36.0	36.0	32.0	36.0
13	34.18126881499251	36.0	36.0	36.0	32.0	36.0
14	34.08986721444992	36.0	36.0	36.0	32.0	36.0
15	34.08495273145741	36.0	36.0	36.0	32.0	36.0
16	34.06416068802762	36.0	36.0	36.0	32.0	36.0
17	34.03691421614274	36.0	36.0	36.0	32.0	36.0
18	34.09786158836757	36.0	36.0	36.0	32.0	36.0
19	34.01747587928384	36.0	36.0	36.0	32.0	36.0
20	33.946781541780055	36.0	36.0	36.0	32.0	36.0
21	33.915555950888525	36.0	36.0	36.0	32.0	36.0
22	33.89064719404703	36.0	36.0	36.0	32.0	36.0
23	33.91976856343102	36.0	36.0	36.0	32.0	36.0
24	33.88240820785372	36.0	36.0	36.0	32.0	36.0
25	33.48416691452191	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	33.0
5	76.0
6	124.0
7	20.0
8	94.0
9	146.0
10	85.0
11	24.0
12	52.0
13	37.0
14	258.0
15	270.0
16	380.0
17	537.0
18	743.0
19	1131.0
20	1739.0
21	2275.0
22	3103.0
23	4258.0
24	5402.0
25	6870.0
26	9066.0
27	11205.0
28	14700.0
29	18876.0
30	24169.0
31	32906.0
32	46226.0
33	67140.0
34	153969.0
35	313589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.21725303562692	22.558155050482586	12.2624594137793	23.962132500111196
2	13.948947028998399	22.496136141256002	41.78290673367728	21.772010096068314
3	17.04687762780606	28.275853441913412	31.829446438970965	22.847822491309564
4	9.773252969173969	18.673963569236243	42.254709532494104	29.29807392909568
5	10.293489942870332	41.296867114082765	36.986767995706586	11.42287494734032
6	26.322917593663792	42.499666281035864	19.81595399127881	11.361462134021535
7	25.070582377631712	35.548901418323325	23.2085983939206	16.171917810124363
8	24.197416486521828	36.876413684887034	21.496217653916027	17.429952174675115
9	25.996722342328805	16.012387208787906	23.16856008436212	34.822330364521164
10	14.512333765169341	28.051575412899698	35.63273252003445	21.803358301896505
11	31.003830863147876	24.607277387610157	27.46087384868856	16.928017900553407
12	22.064384318517416	28.803430341712026	31.839772646615515	17.292412693155043
13	25.400246862384655	23.230390512914354	29.589392988495987	21.779969636205
14	19.98266043963996	25.4204077997406	28.649935707248105	25.946996053371336
15	22.680634712142126	31.3838550247117	26.274683868382386	19.66082639476379
16	21.482241102462563	29.780487058253858	28.260899813825862	20.476372025457714
17	18.46880257611665	29.714403403279427	30.152615985783488	21.664178034820438
18	18.793707875176224	28.752388494034502	33.08013788899574	19.37376574179353
19	21.773305777647884	28.889909469831395	29.519236791015175	19.81754796150554
20	20.736104288630585	29.89433929707036	30.25934393790329	19.110212476395763
21	22.222175834116133	27.33855199526841	28.46912291688411	21.970149253731343
22	20.543994209273514	30.548170213950637	28.50443352496555	20.4034020518103
23	20.950433698181698	29.919050429244287	29.724192452872206	19.406323419701813
24	20.95759131117334	30.664465883217453	29.02473376272734	19.353209042881875
25	20.702535697386367	29.72193614830072	30.418905001809225	19.156623152503688
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	345.0
2	768.5
3	1192.0
4	1192.0
5	1192.0
6	3685.5
7	6179.0
8	6179.0
9	6179.0
10	5977.5
11	5776.0
12	5776.0
13	5776.0
14	6035.5
15	6295.0
16	6295.0
17	6295.0
18	10221.0
19	14147.0
20	14147.0
21	14147.0
22	21803.5
23	29460.0
24	29460.0
25	29460.0
26	43461.0
27	57462.0
28	57462.0
29	57462.0
30	68258.5
31	79055.0
32	79055.0
33	79055.0
34	83816.5
35	88578.0
36	88578.0
37	88578.0
38	84616.0
39	80654.0
40	80654.0
41	80654.0
42	75997.5
43	71341.0
44	71341.0
45	71341.0
46	83235.0
47	95129.0
48	95129.0
49	95129.0
50	82126.0
51	69123.0
52	69123.0
53	69123.0
54	56928.5
55	44734.0
56	44734.0
57	44734.0
58	35624.5
59	26515.0
60	26515.0
61	26515.0
62	21827.5
63	17140.0
64	17140.0
65	17140.0
66	14403.0
67	11666.0
68	11666.0
69	11666.0
70	9313.5
71	6961.0
72	6961.0
73	6961.0
74	5614.0
75	4267.0
76	4267.0
77	4267.0
78	3097.0
79	1927.0
80	1927.0
81	1927.0
82	1366.5
83	806.0
84	806.0
85	806.0
86	558.5
87	311.0
88	311.0
89	311.0
90	208.0
91	105.0
92	105.0
93	105.0
94	68.5
95	32.0
96	32.0
97	32.0
98	169.0
99	306.0
100	306.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006949212376269274
2	0.0025017164554569384
3	0.005142417158439262
4	0.015844204217893943
5	0.03683082559422715
6	0.04697667566358029
7	0.06782431279238811
8	0.0889499184162467
9	0.09728897326776983
10	0.12077731109955997
11	0.12077731109955997
12	0.1357876098323016
13	0.12327902755501692
14	0.12758753922830385
15	0.11368911447576532
16	0.11452301996091763
17	0.12578074401047384
18	0.1330079248817939
19	0.13356386187189545
20	0.12300105905996615
21	0.12939433444613388
22	0.15510642023833018
23	0.14370971194124857
24	0.13537065708972545
25	0.13425878310952236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	719506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.405938651116752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54802420614222	22.3629671192281
2	13.818027752900688	8.403002082682539
3	4.8222148952326185	4.398719108008332
4	2.1810644672118453	2.6526924953669604
5	1.1379390150823137	1.7300051940652525
6	0.750243977318133	1.3687123408622988
7	0.48980727188613304	1.0425134901888435
8	0.38275282828891305	0.9310367212395291
9	0.2955194161124637	0.8086990712876474
>10	1.9503762609160917	11.568198823178028
>50	0.2538271435660866	5.425622484052518
>100	0.30147700444971187	19.29142722537808
>500	0.04719168914638347	9.639235631753722
>1k	0.02153407174640799	10.37716821270816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3707	0.5152146055766039	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3210	0.4461394345564874	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	3062	0.4255697659227303	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2554	0.3549657681798345	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2469	0.34315210714017674	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2371	0.32953165088268893	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2237	0.3109077617142873	No Hit
GTACATGGAAGCAGTGGTATCAACG	2189	0.30423651783306876	No Hit
CCATTGGGATGTCCTGATCCAACAT	2015	0.2800532587636517	No Hit
TCCATGTACTCTGCGTTGATACCAC	1946	0.2704633456844001	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1745	0.24252751193179767	No Hit
CTTCTACACCATTGGGATGTCCTGA	1697	0.23585626805057913	No Hit
GATATATTTTGATCAACGGACCAAG	1643	0.22835111868420832	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1606	0.22320870152576908	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1574	0.2187612056049567	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1545	0.21473066242672056	No Hit
ATCTATAACTTTATAGATGCAACAC	1451	0.20166614315933434	No Hit
ATCGTAAATAGATAGAAACCGACCT	1449	0.20138817466428355	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1431	0.19888645820882658	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1399	0.19443896228801427	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1385	0.19249318282265887	No Hit
GTACATGGGAGAAATCGTAAATAGA	1382	0.19207623008008273	No Hit
GTTATATAATTTAAGCTCCATAGGG	1377	0.1913813088424558	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1371	0.1905474033573035	No Hit
GTTATAGATTAACCCAATTTTAAGT	1363	0.1894355293771004	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1319	0.18332022248598345	No Hit
GTACATGGGGTGGTATCAACGCAAA	1312	0.18234733275330575	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	1310	0.18206936425825496	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1307	0.18165241151567882	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1306	0.18151342726815342	No Hit
ATAAATAATCCACCTATAACTTCTC	1269	0.17637101010971418	No Hit
CCTATAACTTCTCTGTTAACCCAAC	1232	0.1712285929512749	No Hit
GTATCAACGCAGAGTACATGGGAGA	1221	0.16969976622849567	No Hit
CTATAGAACTAGTACCGCAAGGGAA	1219	0.16942179773344487	No Hit
CACTATAAATAATCCACCTATAACT	1215	0.16886586074334334	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	1189	0.16525227030768333	No Hit
GTCCTACAGTGGACATTTCTAAATT	1185	0.1646963333175818	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1185	0.1646963333175818	No Hit
GATAGAAACCGACCTGGATTGCTCC	1173	0.16302852234727716	No Hit
GATTAAAGATAAGAGACAGTTGGAC	1167	0.16219461686212486	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1164	0.1617776641195487	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1139	0.15830305793141405	No Hit
GATTAAACCTTGTACCTTTTGCATA	1129	0.1569132154561602	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	1100	0.15288267227792401	No Hit
GACTATAGGCAATAATCACACTATA	1098	0.15260470378287325	No Hit
CTGTTAACCCAACACCGGAATGCCT	1051	0.14607244414918014	No Hit
AAATATATCTGGGTCAATAAGATAT	1021	0.14190291672341857	No Hit
GACCTGGATTGCTCCGGTCTGAACT	995	0.13828932628775853	No Hit
ACCTTTGCACGGTCAGGATACCGCG	983	0.13662151531745392	No Hit
CCATTAATAGCTTCTACACCATTGG	958	0.13314690912931929	No Hit
AGCATGAACGGCTAAACGAGGGTCC	928	0.12897738170355771	No Hit
GGATACCGCGGCCGTTAAACTTTAG	919	0.12772652347582925	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	916	0.1273095707332531	No Hit
CCTATAGTCTGATTAACTAACAATG	914	0.1270316022382023	No Hit
CTTAAATAGGATTGCGCTGTTATCC	914	0.1270316022382023	No Hit
GTCCTGATCCAACATCGAGGTCGTA	889	0.12355699605006767	No Hit
GTTAGTATGAGTAACAAGAATTCCA	885	0.12300105905996615	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	883	0.12272309056491537	No Hit
GAGTACATGGAAGCAGTGGTATCAA	879	0.12216715357481382	No Hit
AGGGATAACAGCGCAATCCTATTTA	873	0.12133324808966152	No Hit
ATTTAAGAGTTCATATCGACAATTA	861	0.11966543711935688	No Hit
GTTATACGCGTATGCCTGGAGAATT	860	0.11952645287183152	No Hit
GTTTAAAATTGAACTTAAATTCATT	856	0.11897051588172997	No Hit
TACTAACAGTGTTGCATCTATAAAG	848	0.11785864190152688	No Hit
GTACATGGGTGGTATCAACGCAAAA	848	0.11785864190152688	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	838	0.11646879942627303	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	823	0.11438403571339224	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	814	0.11313317748566377	No Hit
CATGTACTCTGCGTTGATACCACTG	808	0.11229927200051146	No Hit
CAGTTGGACCCTCGTTTAGCCGTTC	801	0.11132638226783376	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	786	0.10924161855495299	No Hit
ATTATAACCTAGACTTACAAGTCAA	770	0.1070178705945468	No Hit
CCAATAAAGAAAGCGTTCAAGCTCA	770	0.1070178705945468	No Hit
GTTTAGATTATAGCCAAAAGAGGGA	762	0.10590599661434374	No Hit
ATTTCAGCCTCTTCACTGAAAGGTC	757	0.1052110753767168	No Hit
GATAAAAGGAACTCGGCAAACAAGA	748	0.10396021714898834	No Hit
CCTTTAGGCATTCCGGTGTTGGGTT	748	0.10396021714898834	No Hit
TTGTAGAACAGTGTATATCAATGAG	744	0.1034042801588868	No Hit
AATCTAAACTTACTTTTTGATTTTG	734	0.10201443768363294	No Hit
ACGTAGGACTTTAATCGTTGAACAA	725	0.10076357945590446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.3898424752538548E-4	0.0
8	0.0	0.0	0.0	1.3898424752538548E-4	0.0
9	0.0	0.0	0.0	4.1695274257615644E-4	0.0
10	0.0	0.0	0.0	4.1695274257615644E-4	0.0
11	0.0	0.0	0.0	4.1695274257615644E-4	0.0
12	0.0	0.0	0.0	5.559369901015419E-4	0.0
13	0.0	0.0	0.0	5.559369901015419E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAATG	35	0.0021671343	16.287445	15
TTGCTAA	70	2.4951078E-8	16.28631	10
TCTGTAC	35	0.0021752089	16.27838	3
GACTTAA	55	1.1262329E-5	15.546024	7
GTTCTAA	55	1.1329377E-5	15.536293	1
CCGGCCA	40	0.0052741645	14.250523	9
ACTGTGC	40	0.0052741645	14.250523	8
TAGCCTA	40	0.005276601	14.249531	5
TAGACTG	60	2.5652682E-5	14.24953	5
AAATGTC	175	0.0	14.114802	7
TCCAACG	110	1.8371793E-10	13.823499	18
CCAACGA	55	1.9514638E-4	13.823499	19
CATTCCG	125	7.2759576E-12	13.680501	9
TAGAAAT	190	0.0	13.497677	4
ATGTCCA	185	0.0	13.351841	9
CGTCTGC	50	0.0014977654	13.3004875	10
CTGTGCA	50	0.0014977654	13.3004875	9
CTGTACT	50	0.001500124	13.29771	4
ACTATAT	50	0.0015032738	13.29401	3
CTCTAGG	50	0.0015048509	13.292161	1
>>END_MODULE