FastQCFastQC Report
Thu 2 Feb 2017
SRR4062434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568091
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28400.18111193801890324No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27890.17785957575166236No Hit
GCGCAAGACGGACCAGAGCGAAAGC26910.17160993845382697No Hit
GATTAAGAGGGACGGCCGGGGGCAT25830.16472258306437573No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25630.163447146881144No Hit
GAATAGGACCGCGGTTCTATTTTGT24690.15745259681995497No Hit
GAACTACGACGGTATCTGATCGTCT24460.1559858452092385No Hit
GAATAACGCCGCCGCATCGCCAGTC23410.149289805247272No Hit
GGGTAGGCACACGCTGAGCCAGTCA22580.1439967450868604No Hit
GTATCTGATCGTCTTCGAACCTCCG22520.14361411423189088No Hit
GTCCTATTCCATTATTCCTAGCTGC21610.13781087959818658No Hit
CTATTGGAGCTGGAATTACCGCGGC21070.13436720190346096No Hit
TCGTAGTTCCGACCATAAACGATGC20700.1320076449644823No Hit
GTGCATGGCCGTTCTTAGTTGGTGG20330.12964808802550362No Hit
ATCAGATACCGTCGTAGTTCCGACC20110.12824510822394874No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC19870.1267145848040707No Hit
TATCAACGCAGAGTACTTTTTTTTT19730.1258217794758085No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG19240.1226969608268908No Hit
CTCTTAATCATGGCCTCAGTTCCGA18870.12033740388791211No Hit
CTTTAATATACGCTATTGGAGCTGG18740.1195083703688115No Hit
GCTTTGAACACTCTAATTTTTTCAA18160.11580960543743954No Hit
GTACATGGGGAATAATTGCAATCCC17240.10994259899457365No Hit
GAATAATGGAATAGGACCGCGGTTC17210.1097512835670889No Hit
GAATAATTGCAATCCCCGATCCCCA16990.108348303765534No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16740.10675400853649437No Hit
CATCTAAGGGCATCACAGACCTGTT16480.10509594149829314No Hit
ATATTAAAGTTGCTGCAGTTAAAAA16010.10209866646769862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA851.7300408E-814.5207481
ATCGCCA4600.014.0463116
TCGCCAG4600.013.63449117
CGCCAGT4650.013.2843718
TTAGAAC655.4732955E-513.148523
CATCGCC5100.012.482511515
CGACCAT3750.012.41385610
AAACGCT2000.012.35407119
GCATCGC4850.012.34151414
CGCATCG4850.012.34111913
GTGTTCG550.00306896612.0906449
GCTCGTA2050.012.0485169
CCGACCA3900.011.93563659
AGAATTT5750.011.89919317
ATCCCCG3300.011.80352612
CGTCGTA3550.011.77514310
CGAGCCG4600.011.77373815
TAGAGAG1052.7216265E-711.7612725
CGGTCCA5400.011.61159910
AGGCCCG4450.011.52857410
Produced by FastQC (version 0.11.5)