##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062434_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568091
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20112735804236	32.0	32.0	32.0	32.0	32.0
2	30.842992530407994	32.0	32.0	32.0	32.0	32.0
3	30.825615350129553	32.0	32.0	32.0	32.0	32.0
4	30.907813385830288	32.0	32.0	32.0	32.0	32.0
5	30.694883141348303	32.0	32.0	32.0	32.0	32.0
6	34.357289851162975	36.0	36.0	36.0	32.0	36.0
7	34.228551786854204	36.0	36.0	36.0	32.0	36.0
8	34.23700537787666	36.0	36.0	36.0	32.0	36.0
9	34.39600316563261	36.0	36.0	36.0	32.0	36.0
10	34.059441703319514	36.0	36.0	36.0	32.0	36.0
11	34.37344643901406	36.0	36.0	36.0	32.0	36.0
12	34.13739317424818	36.0	36.0	36.0	32.0	36.0
13	34.288267071235026	36.0	36.0	36.0	32.0	36.0
14	34.17771290059059	36.0	36.0	36.0	32.0	36.0
15	34.11062368191642	36.0	36.0	36.0	32.0	36.0
16	34.105293634106694	36.0	36.0	36.0	32.0	36.0
17	34.02897408377447	36.0	36.0	36.0	32.0	36.0
18	34.0516385847505	36.0	36.0	36.0	32.0	36.0
19	34.03124053387208	36.0	36.0	36.0	32.0	36.0
20	34.03564780360323	36.0	36.0	36.0	32.0	36.0
21	34.00322557810739	36.0	36.0	36.0	32.0	36.0
22	33.96040663456394	36.0	36.0	36.0	32.0	36.0
23	33.93324367016965	36.0	36.0	36.0	32.0	36.0
24	33.89915317414614	36.0	36.0	36.0	32.0	36.0
25	33.46165624316446	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	62.0
5	148.0
6	263.0
7	70.0
8	236.0
9	321.0
10	192.0
11	66.0
12	103.0
13	100.0
14	277.0
15	323.0
16	459.0
17	650.0
18	829.0
19	1209.0
20	1917.0
21	2862.0
22	4327.0
23	6541.0
24	9564.0
25	13377.0
26	18578.0
27	24003.0
28	32145.0
29	43490.0
30	55606.0
31	76654.0
32	109236.0
33	159473.0
34	356518.0
35	648485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22114863356612	17.624732931534805	11.020759590548169	26.1333588443509
2	17.51224782115694	19.958432023795403	35.886211651022876	26.643108504024788
3	19.092246037050455	23.534142334556105	28.05333336734642	29.320278261047022
4	12.693032831845539	14.787278010648855	35.61613605226971	36.9035531052359
5	15.284865179699795	35.97383401030028	32.8830498408006	15.858250969199334
6	34.94532207023275	34.920694671294406	16.49780522662311	13.636178031849736
7	30.821456673190774	30.12892714719571	20.519537539350445	18.530078640263074
8	28.873961337300962	31.60987771577823	19.483390971214252	20.032769975706557
9	27.66984248575669	13.86316847800067	18.750035907502273	39.716953128740364
10	16.63791298040578	25.58767341601455	30.512662689250764	27.261750914328914
11	38.356376926524845	20.858506335870747	21.711291679915615	19.073825057688794
12	25.158723164577818	23.66166134715767	27.64803625184714	23.53157923641737
13	29.23636256831247	18.750387095991506	25.353334835957497	26.659915499738528
14	23.856635044610787	19.8143031643553	24.266442585759158	32.06261920527476
15	25.781511875353956	26.771087460502212	21.75859565695139	25.688805007192446
16	26.64406056782979	25.18049550389339	23.028851187937512	25.146592740339308
17	24.380500911816842	25.41108381052599	24.686355439996323	25.522059837660844
18	25.690023671820573	23.790083889049598	25.925975592545836	24.593916846583998
19	25.964652144121985	24.58385457276247	24.903654518994188	24.54783876412136
20	26.015504331341393	23.609593759956784	24.34918139659022	26.0257205121116
21	27.690889074501207	23.637792882193224	23.80784527084499	24.863472772460582
22	26.433952960773393	23.66792022850252	24.36634237567	25.531784435054085
23	24.51977422778776	23.409625335126634	25.352816979664404	26.717783457421195
24	25.044414160933165	24.746127251200875	24.712217949323794	25.497240638542166
25	24.985504247830107	24.18166051076134	24.832500625807803	26.00033461560075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	210.0
1	210.0
2	517.0
3	824.0
4	824.0
5	824.0
6	2081.5
7	3339.0
8	3339.0
9	3339.0
10	3434.0
11	3529.0
12	3529.0
13	3529.0
14	3920.0
15	4311.0
16	4311.0
17	4311.0
18	6890.5
19	9470.0
20	9470.0
21	9470.0
22	15678.0
23	21886.0
24	21886.0
25	21886.0
26	32414.0
27	42942.0
28	42942.0
29	42942.0
30	51535.5
31	60129.0
32	60129.0
33	60129.0
34	77019.5
35	93910.0
36	93910.0
37	93910.0
38	105666.0
39	117422.0
40	117422.0
41	117422.0
42	135233.0
43	153044.0
44	153044.0
45	153044.0
46	175585.0
47	198126.0
48	198126.0
49	198126.0
50	201765.5
51	205405.0
52	205405.0
53	205405.0
54	191333.0
55	177261.0
56	177261.0
57	177261.0
58	165821.0
59	154381.0
60	154381.0
61	154381.0
62	140763.0
63	127145.0
64	127145.0
65	127145.0
66	107874.5
67	88604.0
68	88604.0
69	88604.0
70	66692.0
71	44780.0
72	44780.0
73	44780.0
74	36266.5
75	27753.0
76	27753.0
77	27753.0
78	23674.0
79	19595.0
80	19595.0
81	19595.0
82	13652.0
83	7709.0
84	7709.0
85	7709.0
86	5811.0
87	3913.0
88	3913.0
89	3913.0
90	2658.5
91	1404.0
92	1404.0
93	1404.0
94	847.0
95	290.0
96	290.0
97	290.0
98	499.5
99	709.0
100	709.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008991825091783576
2	0.0036349931222103816
3	0.004272711213826239
4	0.0142211134430336
5	0.03571221313048797
6	0.04661719249711911
7	0.06900109751283566
8	0.09010956634532052
9	0.09986665314704313
10	0.12212301454443651
11	0.12301581987269872
12	0.13755579236154025
13	0.12384485339179932
14	0.12690590023155543
15	0.11887065227719565
16	0.11778653152144869
17	0.1267145848040707
18	0.13353816838436036
19	0.13468606094926888
20	0.12422748424676885
21	0.1341121146668146
22	0.15426400636187568
23	0.1444431477509915
24	0.13704561788824757
25	0.13487737637675362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1568091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.91533811184455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35757706926559	35.194570676909805
2	12.759101829078462	11.461587453130267
3	3.665804081551047	4.939524893239351
4	1.5654189906375335	2.812452930047489
5	0.8335969840101722	1.8720645192915382
6	0.5390353748470733	1.45265736693007
7	0.3909524119336182	1.2291831837347713
8	0.27243160693521246	0.9789086190278561
9	0.21197609801959513	0.8568880302761656
>10	1.1289792704626127	9.277156289313691
>50	0.11505400992618098	3.625723029828697
>100	0.1281146810009477	12.479641155769691
>500	0.022583365248012778	7.085831155267409
>1k	0.009374227084080776	6.733810697233291
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2840	0.18111193801890324	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2789	0.17785957575166236	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2691	0.17160993845382697	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2583	0.16472258306437573	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2563	0.163447146881144	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2469	0.15745259681995497	No Hit
GAACTACGACGGTATCTGATCGTCT	2446	0.1559858452092385	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2341	0.149289805247272	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2258	0.1439967450868604	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2252	0.14361411423189088	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2161	0.13781087959818658	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2107	0.13436720190346096	No Hit
TCGTAGTTCCGACCATAAACGATGC	2070	0.1320076449644823	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2033	0.12964808802550362	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2011	0.12824510822394874	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1987	0.1267145848040707	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1973	0.1258217794758085	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1924	0.1226969608268908	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1887	0.12033740388791211	No Hit
CTTTAATATACGCTATTGGAGCTGG	1874	0.1195083703688115	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1816	0.11580960543743954	No Hit
GTACATGGGGAATAATTGCAATCCC	1724	0.10994259899457365	No Hit
GAATAATGGAATAGGACCGCGGTTC	1721	0.1097512835670889	No Hit
GAATAATTGCAATCCCCGATCCCCA	1699	0.108348303765534	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1674	0.10675400853649437	No Hit
CATCTAAGGGCATCACAGACCTGTT	1648	0.10509594149829314	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1601	0.10209866646769862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.377180916158565E-5
2	0.0	0.0	0.0	0.0	6.377180916158565E-5
3	0.0	0.0	0.0	0.0	6.377180916158565E-5
4	0.0	0.0	0.0	0.0	6.377180916158565E-5
5	0.0	0.0	0.0	0.0	6.377180916158565E-5
6	0.0	0.0	0.0	0.0	1.275436183231713E-4
7	0.0	0.0	0.0	0.0	1.275436183231713E-4
8	0.0	0.0	0.0	0.0	1.275436183231713E-4
9	0.0	0.0	0.0	6.377180916158565E-5	1.275436183231713E-4
10	0.0	0.0	0.0	6.377180916158565E-5	1.275436183231713E-4
11	0.0	0.0	0.0	6.377180916158565E-5	1.275436183231713E-4
12	0.0	0.0	0.0	1.275436183231713E-4	4.4640266413109955E-4
13	0.0	0.0	0.0	1.275436183231713E-4	5.739462824542708E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAA	85	1.7300408E-8	14.520748	1
ATCGCCA	460	0.0	14.04631	16
TCGCCAG	460	0.0	13.634491	17
CGCCAGT	465	0.0	13.28437	18
TTAGAAC	65	5.4732955E-5	13.14852	3
CATCGCC	510	0.0	12.4825115	15
CGACCAT	375	0.0	12.413856	10
AAACGCT	200	0.0	12.354071	19
GCATCGC	485	0.0	12.341514	14
CGCATCG	485	0.0	12.341119	13
GTGTTCG	55	0.003068966	12.090644	9
GCTCGTA	205	0.0	12.048516	9
CCGACCA	390	0.0	11.9356365	9
AGAATTT	575	0.0	11.899193	17
ATCCCCG	330	0.0	11.803526	12
CGTCGTA	355	0.0	11.775143	10
CGAGCCG	460	0.0	11.773738	15
TAGAGAG	105	2.7216265E-7	11.761272	5
CGGTCCA	540	0.0	11.611599	10
AGGCCCG	445	0.0	11.528574	10
>>END_MODULE