##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568091
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31643635477788	32.0	32.0	32.0	32.0	32.0
2	31.438598907843996	32.0	32.0	32.0	32.0	32.0
3	31.5142577822333	32.0	32.0	32.0	32.0	32.0
4	31.610876537139745	32.0	32.0	32.0	32.0	32.0
5	31.542786738779828	32.0	32.0	32.0	32.0	32.0
6	35.14561591132147	36.0	36.0	36.0	36.0	36.0
7	35.15949712102167	36.0	36.0	36.0	36.0	36.0
8	35.09731514306249	36.0	36.0	36.0	36.0	36.0
9	35.229514103454456	36.0	36.0	36.0	36.0	36.0
10	35.103331375538794	36.0	36.0	36.0	36.0	36.0
11	35.23118556257258	36.0	36.0	36.0	36.0	36.0
12	35.13691616111565	36.0	36.0	36.0	36.0	36.0
13	35.189573819376555	36.0	36.0	36.0	36.0	36.0
14	35.143760789392964	36.0	36.0	36.0	36.0	36.0
15	35.111738413140564	36.0	36.0	36.0	36.0	36.0
16	35.11730059033564	36.0	36.0	36.0	36.0	36.0
17	35.08567806332668	36.0	36.0	36.0	36.0	36.0
18	35.10682096893611	36.0	36.0	36.0	36.0	36.0
19	35.08675899549197	36.0	36.0	36.0	36.0	36.0
20	35.07909489946693	36.0	36.0	36.0	36.0	36.0
21	35.08049532839612	36.0	36.0	36.0	36.0	36.0
22	35.06426667840068	36.0	36.0	36.0	36.0	36.0
23	35.02347440295238	36.0	36.0	36.0	36.0	36.0
24	35.0034915065516	36.0	36.0	36.0	36.0	36.0
25	34.967184940159726	36.0	36.0	36.0	32.0	36.0
26	34.92527155630636	36.0	36.0	36.0	32.0	36.0
27	34.91747800350873	36.0	36.0	36.0	32.0	36.0
28	34.9055992286162	36.0	36.0	36.0	32.0	36.0
29	34.87351244283654	36.0	36.0	36.0	32.0	36.0
30	34.85993733782032	36.0	36.0	36.0	32.0	36.0
31	34.86545997649371	36.0	36.0	36.0	32.0	36.0
32	34.83123683510715	36.0	36.0	36.0	32.0	36.0
33	34.811009692677274	36.0	36.0	36.0	32.0	36.0
34	34.796937167549586	36.0	36.0	36.0	32.0	36.0
35	34.76974550584118	36.0	36.0	36.0	32.0	36.0
36	34.74959170099184	36.0	36.0	36.0	32.0	36.0
37	34.74455117719571	36.0	36.0	36.0	32.0	36.0
38	34.70294644889869	36.0	36.0	36.0	32.0	36.0
39	34.70686395113549	36.0	36.0	36.0	32.0	36.0
40	34.701892938611344	36.0	36.0	36.0	32.0	36.0
41	34.65822327913367	36.0	36.0	36.0	32.0	36.0
42	34.628907378462095	36.0	36.0	36.0	32.0	36.0
43	34.64108779401195	36.0	36.0	36.0	32.0	36.0
44	34.58969281757245	36.0	36.0	36.0	32.0	36.0
45	34.55269687792354	36.0	36.0	36.0	32.0	36.0
46	34.564716588514315	36.0	36.0	36.0	32.0	36.0
47	34.5387499832599	36.0	36.0	36.0	32.0	36.0
48	34.49810055666412	36.0	36.0	36.0	32.0	36.0
49	34.492264798407746	36.0	36.0	36.0	32.0	36.0
50	33.97417751903429	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	13.0
21	32.0
22	131.0
23	343.0
24	921.0
25	2261.0
26	4640.0
27	8822.0
28	14461.0
29	22490.0
30	33309.0
31	48331.0
32	72028.0
33	120925.0
34	273482.0
35	965897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78171172349131	18.239865802204953	11.605047725047598	26.37337474925614
2	16.929726158353546	20.34957395395348	35.72492240228619	26.995777485406787
3	18.402093774731725	23.840857304838984	28.469010131517773	29.288038788911514
4	12.339134893283777	15.284704135747829	35.80050558515998	36.57565538580842
5	14.89008921681791	36.27731826617095	32.81603734479526	16.01655517221588
6	35.005248450017284	35.302681875055	16.2013848765562	13.490684798371513
7	30.84176874939018	30.251178024744735	20.4589529561741	18.448100269690983
8	28.811146802066972	31.850511226708143	19.303407774166168	20.034934197058714
9	27.77185762816061	13.748245478100444	18.626597563534258	39.85329933020469
10	16.61370417915797	25.75296969372313	30.44549072726009	27.187835399858812
11	38.58162568371351	20.730939722248262	21.580826622944716	19.106607971093513
12	25.1618687946044	23.55698744524393	27.677220263364816	23.60392349678686
13	29.385539487185376	18.651022166443145	25.371741818555172	26.591696527816307
14	23.94994933329762	19.768686893809097	24.12525803668282	32.15610573621046
15	25.743276378730574	26.831287214836387	21.675336444122184	25.750099962310863
16	26.692455986291613	25.198728900299795	22.96939399562908	25.139421117779513
17	24.515860367797533	25.45330596247284	24.52746683706494	25.503366832664685
18	25.857619232557294	23.69530849931541	25.752587062868166	24.694485205259134
19	25.989371790285134	24.600294243127472	24.895557719545614	24.514776247041787
20	26.220735913923367	23.48256574395236	24.303245156052807	25.99345318607147
21	27.838307853306983	23.577394424175637	23.704874270689647	24.87942345182773
22	26.443171984278973	23.685041238040395	24.370715730145765	25.501071047534868
23	24.533716474362777	23.337739965346397	25.289603728355054	26.838939831935775
24	25.139421117779513	24.664703770380672	24.64595485848717	25.549920253352642
25	25.169776498940433	23.9611093999009	24.884907827415628	25.984206273743045
26	25.044146034892105	24.96781117932569	25.204085732269366	24.78395705351284
27	26.104288590394308	23.975394285153094	24.26644882216657	25.653868302286025
28	24.92808134221802	23.872976759639585	25.503239289046363	25.695702609096028
29	24.775347859276025	24.241769132021037	25.364535604119915	25.618347404583027
30	24.647485381907046	24.61343123581476	25.690409548935616	25.048673833342583
31	25.597876653842157	24.37422317964965	24.08788775651413	25.94001240999406
32	25.22187806702545	24.127107419148505	24.136290559667774	26.51472395415827
33	24.661834038968404	23.662529789406356	24.985284655035965	26.690351516589278
34	25.829113233862067	23.939235669358474	25.168437290948038	25.06321380583142
35	26.61510078177861	23.596079564259984	25.13183227248929	24.65698738147212
36	24.84065019185749	24.615663249135412	24.56005423154651	25.983632327460587
37	26.316010996810775	24.27014758709794	24.303755330526098	25.110086085565186
38	25.030562639540687	23.82495658734091	25.217732899429947	25.926747873688456
39	25.86036142035124	23.932284542159863	24.165306732836296	26.0420473046526
40	26.024191198087355	24.125130493064496	25.062958718594775	24.78771959025337
41	24.643977932403157	24.750413081893843	25.52606959672621	25.07953938897679
42	26.472251929256657	24.83707897054444	24.664384911334867	24.02628418886404
43	25.29642731193534	23.312932731582542	25.13540349380234	26.255236462679783
44	24.812781911253875	24.35968320716081	24.68102935352604	26.146505528059276
45	24.910416551080264	24.619617101303433	24.8693475059802	25.600618841636102
46	24.7149874783893	24.196139377292997	25.071854977619257	26.017018166698445
47	24.934330979515856	23.82444641286762	25.591116842071028	25.650105765545494
48	26.22417959161809	24.804300260635383	24.212242784379225	24.759277363367303
49	24.515541508751724	25.080495966114214	24.586710847776054	25.817251677358012
50	24.61931394243953	25.124386993093506	24.55496814596101	25.701330918505956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	52.0
2	55.0
3	128.0
4	201.0
5	219.5
6	238.0
7	230.5
8	223.0
9	320.0
10	417.0
11	564.0
12	711.0
13	1278.0
14	1845.0
15	2455.0
16	3065.0
17	3535.0
18	4005.0
19	4342.0
20	4679.0
21	5517.5
22	6356.0
23	6579.0
24	6802.0
25	8323.5
26	9845.0
27	12032.0
28	14219.0
29	16168.0
30	18117.0
31	19432.0
32	20747.0
33	23681.5
34	26616.0
35	30926.0
36	35236.0
37	44977.5
38	54719.0
39	59310.5
40	63902.0
41	70891.0
42	77880.0
43	78581.0
44	79282.0
45	88750.5
46	98219.0
47	105164.0
48	112109.0
49	116861.5
50	121614.0
51	114219.0
52	106824.0
53	106422.5
54	106021.0
55	110331.0
56	114641.0
57	112986.0
58	111331.0
59	103121.0
60	94911.0
61	83564.5
62	72218.0
63	63105.0
64	53992.0
65	47049.0
66	40106.0
67	35868.5
68	31631.0
69	29827.5
70	28024.0
71	21473.5
72	14923.0
73	13795.5
74	12668.0
75	8979.5
76	5291.0
77	5001.0
78	4711.0
79	4139.0
80	3567.0
81	2898.5
82	2230.0
83	2005.5
84	1781.0
85	1506.5
86	1232.0
87	919.0
88	606.0
89	383.5
90	161.0
91	99.5
92	38.0
93	26.5
94	15.0
95	11.0
96	7.0
97	6.0
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016325583145365925
2	0.004336483022987824
3	8.928053282621991E-4
4	4.4640266413109955E-4
5	6.377180916158565E-5
6	5.739462824542708E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.275436183231713E-4
47	0.0
48	0.0
49	0.0
50	6.377180916158565E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1568091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.75568780684436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82202625446031	37.64200078110896
2	12.68440447832189	12.115049205649573
3	3.6118330175313225	5.174567099870353
4	1.5081638726306776	2.8809361225164807
5	0.8216262049913046	1.9618662269743525
6	0.5080137823087086	1.4556328553705327
7	0.34263155180663124	1.145382379459647
8	0.24582205208525187	0.9391520940336897
9	0.18923982005938936	0.8133549990641642
>10	1.0287186954860883	8.962085416489433
>50	0.10110487273611167	3.4290292066519594
>100	0.10937335691199505	11.415902026157818
>500	0.019545237316240253	6.433849215797101
>1k	0.007496803354174343	5.6311923708560485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2848	0.18162211249219593	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2694	0.17180125388131173	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2609	0.16638065010257697	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2539	0.16191662346126595	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2474	0.15777145586576288	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2282	0.14552726850673844	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2272	0.14488955041512258	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2236	0.14259376528530548	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2208	0.1408081546287811	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2201	0.14036175196465	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2201	0.14036175196465	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2049	0.130668436972089	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1853	0.11816916237641821	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1813	0.11561829000995479	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1773	0.11306741764349135	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	1735	0.1106440888953511	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1726	0.11007014261289683	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1692	0.10790190110140292	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1670	0.10649892129984803	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1652	0.10535102873493948	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	1635	0.10426690797919254	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1627	0.10375673350589984	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1576	0.10050437123865898	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.275436183231713E-4
2	0.0	0.0	0.0	0.0	1.275436183231713E-4
3	0.0	0.0	0.0	0.0	1.275436183231713E-4
4	0.0	0.0	0.0	0.0	1.275436183231713E-4
5	0.0	0.0	0.0	0.0	1.275436183231713E-4
6	0.0	0.0	0.0	0.0	2.550872366463426E-4
7	0.0	0.0	0.0	0.0	2.550872366463426E-4
8	0.0	0.0	0.0	0.0	2.550872366463426E-4
9	0.0	0.0	0.0	0.0	2.550872366463426E-4
10	0.0	0.0	0.0	0.0	2.550872366463426E-4
11	0.0	0.0	0.0	0.0	2.550872366463426E-4
12	0.0	0.0	0.0	1.275436183231713E-4	6.377180916158565E-4
13	0.0	0.0	0.0	1.275436183231713E-4	0.0010203489465853703
14	0.0	0.0	0.0	2.550872366463426E-4	0.0010203489465853703
15	0.0	0.0	0.0	3.1885904580792826E-4	0.0010203489465853703
16	0.0	0.0	0.0	3.826308549695139E-4	0.001084120755746956
17	0.0	0.0	0.0	4.4640266413109955E-4	0.001084120755746956
18	0.0	0.0	0.0	5.739462824542708E-4	0.0011478925649085416
19	0.0	0.0	0.0	8.290335191006134E-4	0.0011478925649085416
20	0.0	0.0	0.0	0.001275436183231713	0.001275436183231713
21	0.0	0.0	0.0	0.0014667516107164699	0.001275436183231713
22	0.0	0.0	0.0	0.0020406978931707407	0.0013392079923932986
23	0.0	0.0	0.0	0.0031885904580792822	0.0014667516107164699
24	0.0	0.0	0.0	0.004400254832149409	0.0014667516107164699
25	0.0	0.0	0.0	0.005101744732926852	0.0014667516107164699
26	0.0	0.0	0.0	0.006313409106996979	0.0015305234198780555
27	0.0	0.0	0.0	0.008417878809329305	0.0016580670382012267
28	0.0	0.0	0.0	0.014157341633872014	0.0017218388473628126
29	0.0	0.0	0.0	0.025572495473795843	0.0017218388473628126
30	0.0	0.0	0.0	0.04955069571855205	0.0017218388473628126
31	0.0	0.0	0.0	0.09852744515464983	0.0017218388473628126
32	0.0	0.0	0.0	0.15228708027786653	0.0017218388473628126
33	0.0	0.0	0.0	0.21255143993556497	0.0017218388473628126
34	0.0	0.0	0.0	0.28078727573846163	0.0018493824656859838
35	0.0	0.0	0.0	0.354762574365901	0.0018493824656859838
36	0.0	0.0	0.0	0.4670647302994533	0.0018493824656859838
37	0.0	0.0	0.0	0.6182039180124113	0.0018493824656859838
38	0.0	0.0	0.0	0.8153863519400341	0.0019131542748475694
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	175	0.0	22.628458	4
GTCTACG	55	0.0044793636	20.003725	1
AGGACGT	200	0.0	19.7999	5
GTATCAA	2865	0.0	19.35439	1
TAACGGC	255	0.0	18.117554	36
CGTCGTA	650	0.0	17.93837	10
GTGTACG	75	0.0012894915	17.603277	1
CGCAATA	685	0.0	17.02181	36
AGAGCGA	925	0.0	16.8864	15
TTAACGG	275	0.0	16.799915	35
GTATTAG	590	0.0	16.782787	1
GTCTATT	105	3.769472E-5	16.765028	1
GATCGAA	105	3.775661E-5	16.76182	11
CGTCTTA	225	1.8189894E-12	16.622137	15
GAGCGAA	955	0.0	16.355936	16
TAGGGAG	135	1.0860058E-6	16.296213	5
ACCGTCG	730	0.0	16.27389	8
ATACCGT	710	0.0	16.112595	6
TCTAGTA	110	5.621662E-5	16.000431	2
TACCGTC	715	0.0	15.999919	7
>>END_MODULE