FastQCFastQC Report
Thu 2 Feb 2017
SRR4062433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405021
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTG21820.15530016989069914No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21350.15195502415978124No Hit
GCGCAAGACGGACCAGAGCGAAAGC21230.1511009443986958No Hit
GTATCAACGCAGAGTACTTTTTTTT21130.15038921126445795No Hit
GAATAGGACCGCGGTTCTATTTTGT20830.14825401186174442No Hit
GGGTAGGCACACGCTGAGCCAGTCA19580.13935734768377128No Hit
GTACATGGAAGCAGTGGTATCAACG19550.13914382774349993No Hit
GTACATGGGGAATAATTGCAATCCC18710.13316526941590198No Hit
GTCCTATTCCATTATTCCTAGCTGC18680.13295174947563063No Hit
GTATCTGATCGTCTTCGAACCTCCG18440.13124358995345978No Hit
GAACTACGACGGTATCTGATCGTCT18230.12974895037156028No Hit
GAATAACGCCGCCGCATCGCCAGTC17910.12747140434199916No Hit
GATTAAGAGGGACGGCCGGGGGCAT17040.12127932607412986No Hit
CTATTGGAGCTGGAATTACCGCGGC16890.12021172637277308No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG16460.11715127389555031No Hit
TCCATGTACTCTGCGTTGATACCAC16340.1162971941344649No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC16340.1162971941344649No Hit
CTCTTAATCATGGCCTCAGTTCCGA16220.11544311437337947No Hit
ATCAGATACCGTCGTAGTTCCGACC15740.1120267953290378No Hit
CTTTAATATACGCTATTGGAGCTGG15240.10846812965784854No Hit
TCGTAGTTCCGACCATAAACGATGC15040.10704466338937284No Hit
GTGCATGGCCGTTCTTAGTTGGTGG15030.10697349007594903No Hit
TATCAACGCAGAGTACTTTTTTTTT14660.10434007747926899No Hit
GTCCTGTATTGTTATTTTTCGTCAC14560.10362834434503114No Hit
GCTTTGAACACTCTAATTTTTTCAA14170.10085258512150352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC653.383915E-614.6102353
ACTTATC500.00149978113.2995778
CGAACGA1251.0186341E-1012.92327116
TTATACA1251.0186341E-1012.9191284
CGAGACT1550.012.8700994
CGCATCG3000.012.35004613
GTATTAC701.0950773E-412.2091131
TTCTAGG951.0444055E-611.9953452
CGCCAGT3250.011.98935818
ATCGCCA3300.011.80601916
GCATCGC3150.011.76278614
AATACCC1052.7225906E-711.7606935
GGTTCTA3800.011.75004413
AGTGTTC2350.011.7230318
CCGTCAA3000.011.72130318
TCTAGGC658.0463255E-411.6881883
GCTCGTA1552.5465852E-1111.644799
AGAATTT4000.011.64169117
CGTTATT1800.011.6066072
GTATTAT907.4942654E-611.60619451
Produced by FastQC (version 0.11.5)