##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062433_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1405021
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.053130878470856	32.0	32.0	32.0	32.0	32.0
2	30.471071250892336	32.0	32.0	32.0	32.0	32.0
3	30.473143106046102	32.0	32.0	32.0	32.0	32.0
4	30.57952016375556	32.0	32.0	32.0	32.0	32.0
5	30.20648374650628	32.0	32.0	32.0	21.0	32.0
6	33.907131637178374	36.0	36.0	36.0	32.0	36.0
7	33.76571168687158	36.0	36.0	36.0	32.0	36.0
8	33.72492297268155	36.0	36.0	36.0	32.0	36.0
9	34.01833211033856	36.0	36.0	36.0	32.0	36.0
10	33.45072778271641	36.0	36.0	36.0	27.0	36.0
11	33.9988733264485	36.0	36.0	36.0	32.0	36.0
12	33.63110658132512	36.0	36.0	36.0	27.0	36.0
13	33.85222356107133	36.0	36.0	36.0	32.0	36.0
14	33.69606148235507	36.0	36.0	36.0	27.0	36.0
15	33.58428806402182	36.0	36.0	36.0	27.0	36.0
16	33.607007297399825	36.0	36.0	36.0	27.0	36.0
17	33.48633935008801	36.0	36.0	36.0	27.0	36.0
18	33.51419444976267	36.0	36.0	36.0	27.0	36.0
19	33.506144036281306	36.0	36.0	36.0	27.0	36.0
20	33.51571898213621	36.0	36.0	36.0	27.0	36.0
21	33.501071514233594	36.0	36.0	36.0	27.0	36.0
22	33.44205246754319	36.0	36.0	36.0	27.0	36.0
23	33.401707163095786	36.0	36.0	36.0	27.0	36.0
24	33.35334845528999	36.0	36.0	36.0	21.0	36.0
25	32.84060594112116	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	13.0
4	54.0
5	129.0
6	262.0
7	77.0
8	231.0
9	235.0
10	156.0
11	53.0
12	108.0
13	67.0
14	188.0
15	235.0
16	425.0
17	625.0
18	890.0
19	1417.0
20	2456.0
21	3710.0
22	5797.0
23	8733.0
24	12334.0
25	17531.0
26	23654.0
27	29863.0
28	39391.0
29	51502.0
30	63800.0
31	85425.0
32	117890.0
33	159473.0
34	315151.0
35	463142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26091091116029	16.9441832556173	10.745739379500762	26.04916645372165
2	17.398477681246895	19.30180318044189	36.04802122762039	27.251697910690826
3	19.22839848876969	23.18918564957564	27.90786562919055	29.67455023246412
4	12.479169825450077	14.601089255295902	35.21899355999695	37.70074735925707
5	14.9557636799378	36.33124053935132	33.076777391559425	15.636218389151457
6	35.67982112593816	34.672515202871104	16.23075608470883	13.416907586481905
7	31.17705042263392	29.874392116531297	20.614122553865528	18.334434906969257
8	29.033416254199473	31.95025032983128	19.126723599962386	19.88960981600686
9	27.21129678496726	13.895641097248898	18.31702354423021	40.57603857355364
10	15.995393892061447	26.018381677039436	31.191274617808478	26.79494981309064
11	38.42893994242226	20.56850896445572	21.578969871447708	19.423581221674315
12	24.6930349999608	23.408945297550623	28.139224457711205	23.75879524477737
13	29.44126661531149	18.48166356564068	25.39615577293221	26.680914046115618
14	23.820691522718203	19.442092811128216	24.410167607319995	32.32704805883359
15	25.67351013338027	26.741179295597718	21.78945740830864	25.795853162713378
16	26.807336011052897	25.140637280155165	23.023970506607725	25.028056202184217
17	24.490368904005432	25.638290367336293	24.644931052739967	25.226409675918305
18	25.898612519607095	23.901307804853367	25.58910369293988	24.610975982599662
19	25.906264208817415	24.883229355078257	24.50872234313822	24.701784092966108
20	25.84153484040108	23.748253223297386	24.42401797478656	25.986193961514974
21	27.32186812732593	23.838335464174083	23.761867500188856	25.077928908311137
22	26.064215004219793	23.76157592208207	24.426906320567504	25.74730275313063
23	24.374035126732498	23.52059893060325	25.184564308491453	26.920801634172797
24	25.15028824697802	24.808411438084807	24.439523724340532	25.601776590596643
25	25.36077007993991	24.11018123845924	24.436511663871823	26.09253701772903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	137.0
2	377.0
3	617.0
4	617.0
5	617.0
6	1607.5
7	2598.0
8	2598.0
9	2598.0
10	2761.5
11	2925.0
12	2925.0
13	2925.0
14	3151.5
15	3378.0
16	3378.0
17	3378.0
18	5460.0
19	7542.0
20	7542.0
21	7542.0
22	12286.5
23	17031.0
24	17031.0
25	17031.0
26	25457.5
27	33884.0
28	33884.0
29	33884.0
30	41944.5
31	50005.0
32	50005.0
33	50005.0
34	64766.5
35	79528.0
36	79528.0
37	79528.0
38	92065.5
39	104603.0
40	104603.0
41	104603.0
42	122095.5
43	139588.0
44	139588.0
45	139588.0
46	161814.0
47	184040.0
48	184040.0
49	184040.0
50	186971.5
51	189903.0
52	189903.0
53	189903.0
54	178874.0
55	167845.0
56	167845.0
57	167845.0
58	156082.0
59	144319.0
60	144319.0
61	144319.0
62	129655.5
63	114992.0
64	114992.0
65	114992.0
66	95831.5
67	76671.0
68	76671.0
69	76671.0
70	57641.0
71	38611.0
72	38611.0
73	38611.0
74	30198.5
75	21786.0
76	21786.0
77	21786.0
78	18290.5
79	14795.0
80	14795.0
81	14795.0
82	10214.5
83	5634.0
84	5634.0
85	5634.0
86	4181.5
87	2729.0
88	2729.0
89	2729.0
90	1863.0
91	997.0
92	997.0
93	997.0
94	608.5
95	220.0
96	220.0
97	220.0
98	431.5
99	643.0
100	643.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008540797610854215
2	0.004199225492003323
3	0.005195651879936314
4	0.014804049192147306
5	0.03693894966694448
6	0.048469026441597673
7	0.06896694070764778
8	0.09060362798847847
9	0.09715157282346669
10	0.1186459134774498
11	0.12106580613385849
12	0.1315282832071549
13	0.122560445715758
14	0.12391273867080989
15	0.11316556834381836
16	0.11302322171697077
17	0.1211369794472823
18	0.13024716356552676
19	0.13117241664003598
20	0.122560445715758
21	0.12946425711786513
22	0.15010451801076283
23	0.14142137377306105
24	0.13409052249041117
25	0.13110124332661222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1405021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.439625783571714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15928877196023	39.13605614179404
2	12.347711798846866	12.209325012367644
3	3.7244374749777176	5.524043850516272
4	1.5244548816187247	3.0147391548467546
5	0.8191318298347097	2.0248785567220198
6	0.4961694890991784	1.4718260319773608
7	0.3308329992624566	1.1449381776274759
8	0.23046770434781522	0.9115389646523864
9	0.1786589651636195	0.7949549142512571
>10	0.9594787608294938	8.793670518339459
>50	0.10384085743416605	3.589298215270073
>100	0.10330685731652041	11.17447249778995
>500	0.015438299974072051	5.258459400711102
>1k	0.00678130933440548	4.951798563134193
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTG	2182	0.15530016989069914	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2135	0.15195502415978124	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2123	0.1511009443986958	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2113	0.15038921126445795	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2083	0.14825401186174442	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1958	0.13935734768377128	No Hit
GTACATGGAAGCAGTGGTATCAACG	1955	0.13914382774349993	No Hit
GTACATGGGGAATAATTGCAATCCC	1871	0.13316526941590198	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1868	0.13295174947563063	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1844	0.13124358995345978	No Hit
GAACTACGACGGTATCTGATCGTCT	1823	0.12974895037156028	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1791	0.12747140434199916	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1704	0.12127932607412986	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1689	0.12021172637277308	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1646	0.11715127389555031	No Hit
TCCATGTACTCTGCGTTGATACCAC	1634	0.1162971941344649	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1634	0.1162971941344649	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1622	0.11544311437337947	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1574	0.1120267953290378	No Hit
CTTTAATATACGCTATTGGAGCTGG	1524	0.10846812965784854	No Hit
TCGTAGTTCCGACCATAAACGATGC	1504	0.10704466338937284	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1503	0.10697349007594903	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1466	0.10434007747926899	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1456	0.10362834434503114	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1417	0.10085258512150352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.117331342378513E-5
7	0.0	0.0	0.0	7.117331342378513E-5	7.117331342378513E-5
8	0.0	0.0	0.0	7.117331342378513E-5	7.117331342378513E-5
9	0.0	0.0	0.0	7.117331342378513E-5	7.117331342378513E-5
10	0.0	0.0	0.0	7.117331342378513E-5	7.117331342378513E-5
11	0.0	0.0	0.0	7.117331342378513E-5	7.117331342378513E-5
12	0.0	0.0	0.0	7.117331342378513E-5	4.2703988054271073E-4
13	0.0	0.0	0.0	7.117331342378513E-5	4.2703988054271073E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTAC	65	3.383915E-6	14.610235	3
ACTTATC	50	0.001499781	13.299577	8
CGAACGA	125	1.0186341E-10	12.923271	16
TTATACA	125	1.0186341E-10	12.919128	4
CGAGACT	155	0.0	12.870099	4
CGCATCG	300	0.0	12.350046	13
GTATTAC	70	1.0950773E-4	12.209113	1
TTCTAGG	95	1.0444055E-6	11.995345	2
CGCCAGT	325	0.0	11.989358	18
ATCGCCA	330	0.0	11.806019	16
GCATCGC	315	0.0	11.762786	14
AATACCC	105	2.7225906E-7	11.760693	5
GGTTCTA	380	0.0	11.750044	13
AGTGTTC	235	0.0	11.723031	8
CCGTCAA	300	0.0	11.721303	18
TCTAGGC	65	8.0463255E-4	11.688188	3
GCTCGTA	155	2.5465852E-11	11.64479	9
AGAATTT	400	0.0	11.641691	17
CGTTATT	180	0.0	11.606607	2
GTATTAT	90	7.4942654E-6	11.6061945	1
>>END_MODULE