FastQCFastQC Report
Thu 2 Feb 2017
SRR4062433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405021
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22580.1607093417109068No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21380.1521685441000526No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20970.1492504382496774No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20620.14675937227984492No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19830.1411366805193659No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17710.12604793807352344No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA17630.1254785515661332No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17370.12362804541711477No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC17260.12284513896945312No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16740.1191441266714163No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16700.11885943341772116No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16580.11800535365663574No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16180.11515842111968434No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT15710.11181327538876643No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14780.10519415724035441No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT14690.10455359741954035No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14340.10206253144970787No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT14180.1009237584349273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT5050.019.1682246
CGTCGTA5050.018.73258210
TACCGTC5150.017.941667
AGAGCGA7250.017.90336215
ACCGTCG5200.017.7691468
ATACGAA6200.017.74184840
GTCGTAG5350.017.68215811
TTAATAC2250.017.5999153
CCGTCGT5300.017.4338789
TACGAAT6650.017.20292541
ATTACTC902.2157759E-417.1110273
GAGCGAA7400.016.94586416
ACGGACC7400.016.9458648
CATGGGT10400.016.9229974
CGAATGC6500.016.92299543
CAAGACG7700.016.8570614
TATACTG2350.016.8509835
CATTCGA4200.016.76182441
TGTATAC1451.2497185E-716.6895753
GTATATA1854.2200554E-1016.6521241
Produced by FastQC (version 0.11.5)