FastQCFastQC Report
Thu 2 Feb 2017
SRR4062432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580213
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG197293.4003029921770107No Hit
TCCATGTACTCTGCGTTGATACCAC165772.8570542197434388No Hit
GAGTACATGGAAGCAGTGGTATCAA77331.3327864077502571No Hit
CATGTACTCTGCGTTGATACCACTG62891.0839122873841158No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52980.9131129430054135No Hit
GTATCAACGCAGAGTACTTTTTTTT50340.8676124113041245No Hit
GCTTCCATGTACTCTGCGTTGATAC47530.8191819211220707No Hit
CCCATGTACTCTGCGTTGATACCAC47390.8167690141379115No Hit
GTACATGGGAAGCAGTGGTATCAAC44160.7610998030033799No Hit
GCGTTGATACCACTGCTTCCATGTA43540.7504140720735316No Hit
CATGGAAGCAGTGGTATCAACGCAG42890.7392112896470779No Hit
TATCAACGCAGAGTACTTTTTTTTT39040.6728563475826981No Hit
GTATCAACGCAGAGTACATGGAAGC33070.5699630997581923No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32750.5644478837943997No Hit
GTCCTAAAGTGTGTATTTCTCATTT30030.5175685481021625No Hit
ACTCTGCGTTGATACCACTGCTTCC27820.47947908785221977No Hit
ACGCAGAGTACATGGAAGCAGTGGT26880.46327814095857894No Hit
GTCCTACAGTGGACATTTCTAAATT26220.4519030080332567No Hit
CAGTGGTATCAACGCAGAGTACATG25240.43501265914414183No Hit
CTGTAGGACGTGGAATATGGCAAGA25170.43380620565206224No Hit
CTTTAGGACGTGAAATATGGCGAGG24210.41726055776068444No Hit
GTACATGGGGTGGTATCAACGCAAA24160.4163988052663418No Hit
TATCAACGCAGAGTACATGGAAGCA22260.38365221048132325No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21910.37761994302092505No Hit
GTATCAACGCAGAGTACATGGGAAG20270.34935446120648794No Hit
GGTATCAACGCAGAGTACTTTTTTT18770.3235018863762101No Hit
GTCCTACAGTGTGCATTTCTCATTT17610.30350922850746187No Hit
GGTATCAACGCAGAGTACATGGAAG15910.27420964369981365No Hit
GTACATGGGTGGTATCAACGCAAAA15490.2669709227473359No Hit
GAGTACATGGGAAGCAGTGGTATCA14480.24956352236161547No Hit
AAGCAGTGGTATCAACGCAGAGTAC14310.24663356388085067No Hit
CTGTAGGACCTGGAATATGGCGAGA14260.24577181138650808No Hit
ATACCACTGCTTCCATGTACTCTGC13750.23698193594421357No Hit
GCGTTGATACCACTGCTTCCCATGT13430.23146671998042098No Hit
TATCAACGCAGAGTACATGGGAAGC13210.22767500900531357No Hit
GATATACACTGTTCTACAAATCCCG12900.22233214354038947No Hit
GTACTCTGCGTTGATACCACTGCTT12270.2114740621116728No Hit
AAAAAGTACTCTGCGTTGATACCAC12170.2097505571229876No Hit
CTGAAGGACCTGGAATATGGCGAGA12090.20837175313203946No Hit
GTGGTATCAACGCAGAGTACATGGA11780.20302888766711535No Hit
TTGTAGAACAGTGTATATCAATGAG11670.20113303217956166No Hit
GTACATGGGAGTGGTATCAACGCAA11630.20044363018408756No Hit
GCTTCCCATGTACTCTGCGTTGATA11580.199581877689745No Hit
ATTTAGAAATGTCCACTGTAGGACG11530.19872012519540236No Hit
CCACTGCTTCCATGTACTCTGCGTT11170.1925155072361357No Hit
GAAGCAGTGGTATCAACGCAGAGTA10900.1878620437666857No Hit
ACATGGAAGCAGTGGTATCAACGCA10530.1814850753085505No Hit
GTCCTTCAGTGTGCATTTCTCATTT10390.1790721683243912No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10270.17700396233796897No Hit
GGTATCAACGCAGAGTACATGGGAA10180.17545280784815231No Hit
GTGTATATCAATGAGTTACAATGAA9810.1690758393900171No Hit
GTATCAACGCAGAGTACATGGGGTG9750.168041736396806No Hit
ACGCAGAGTACATGGGAAGCAGTGG9410.16218181943527638No Hit
TTTCTAAATTTTCCACCTTTTTCAG9320.1606306649454597No Hit
TGGAAGCAGTGGTATCAACGCAGAG9080.1564942529726152No Hit
CTGCTTCCATGTACTCTGCGTTGAT9020.1554601499794041No Hit
GTTGATACCACTGCTTCCATGTACT8790.15149608850542817No Hit
GATACCACTGCTTCCATGTACTCTG8120.1399486050812374No Hit
GTGTATATCAATGAGTTACAATGAG8070.13908685258689482No Hit
GTTCTACAGTGTGGTTTTTATCATT7790.13426103861857627No Hit
GCAGAGTACATGGAAGCAGTGGTAT7670.13219283263215406No Hit
TATCAACGCAGAGTACATGGGGTGG7650.131848131634417No Hit
CCCCATGTACTCTGCGTTGATACCA7490.12909052365252072No Hit
GAATATGGCAAGAAAACTGAAAATC7070.12185180270004291No Hit
CCATGTACTCTGCGTTGATACCACT6920.11926654521701513No Hit
GCAGTGGTATCAACGCAGAGTACAT6920.11926654521701513No Hit
GGAATATGGCGAGAAAACTGAAAAT6480.11168312326680029No Hit
ATCAACGCAGAGTACTTTTTTTTTT6130.10565085580640213No Hit
CTTCCATGTACTCTGCGTTGATACC6060.1044444023143225No Hit
GTTCTACAAATCCCGTTTCCAACGA5990.10323794882224285No Hit
CTGTAGGACATGGAATATGGCAAGA5950.10254854682676878No Hit
GATATACACTGTTCTACAATGCCGG5940.10237619632790028No Hit
GTATTAGAGGCACTGCCTGCCCAGT5860.10099739233695212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT250.006029036418.9992226
AGTGTAC402.7582288E-416.624328
CCAGGAC350.00216983416.2836443
CCAGTAC350.00216983416.2836443
GAACTCG601.4635625E-615.8326859
AATGTCC3850.015.7915628
AAATGTC3800.015.7493567
ACTGCCT807.676135E-915.43820112
GAAATGT3900.015.3455266
TAACAGT755.826587E-815.1993794
CTGGGTC508.7019514E-515.1993789
GCGTATG508.7019514E-515.1993788
ATGTCCA3900.015.1019479
TTCATGC456.7730754E-414.7720752
TGTCCAC4000.014.72439810
CTCGGCA653.3617252E-614.61604912
TAGGACC5700.014.4994084
AAAGGAA1058.731149E-1114.4755995
TAGAAAT4250.014.3052984
AGTATGG400.00527222814.25064814
Produced by FastQC (version 0.11.5)