##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062432_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580213
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87540437735797	32.0	32.0	32.0	32.0	32.0
2	31.440831211985945	32.0	32.0	32.0	32.0	32.0
3	31.5177150460262	32.0	32.0	32.0	32.0	32.0
4	31.645269926733803	32.0	32.0	32.0	32.0	32.0
5	31.586918941836878	32.0	32.0	32.0	32.0	32.0
6	35.168476059653955	36.0	36.0	36.0	36.0	36.0
7	35.19255687135586	36.0	36.0	36.0	36.0	36.0
8	35.168043459901796	36.0	36.0	36.0	36.0	36.0
9	35.20625528900593	36.0	36.0	36.0	36.0	36.0
10	35.156532170082365	36.0	36.0	36.0	36.0	36.0
11	35.21377838828155	36.0	36.0	36.0	36.0	36.0
12	35.143731698531404	36.0	36.0	36.0	36.0	36.0
13	35.14952439879837	36.0	36.0	36.0	36.0	36.0
14	35.0921247886552	36.0	36.0	36.0	36.0	36.0
15	35.091125155761766	36.0	36.0	36.0	36.0	36.0
16	35.06756311906145	36.0	36.0	36.0	36.0	36.0
17	35.07326102655404	36.0	36.0	36.0	36.0	36.0
18	35.05788908555996	36.0	36.0	36.0	36.0	36.0
19	34.959177060838	36.0	36.0	36.0	36.0	36.0
20	34.91313362506528	36.0	36.0	36.0	36.0	36.0
21	34.76944846116857	36.0	36.0	36.0	32.0	36.0
22	34.73265162966014	36.0	36.0	36.0	32.0	36.0
23	34.625930477255764	36.0	36.0	36.0	32.0	36.0
24	34.61357811700186	36.0	36.0	36.0	32.0	36.0
25	34.560714771988906	36.0	36.0	36.0	32.0	36.0
26	34.47067025385505	36.0	36.0	36.0	32.0	36.0
27	34.38566871131808	36.0	36.0	36.0	32.0	36.0
28	34.26038196317559	36.0	36.0	36.0	32.0	36.0
29	34.19116772633498	36.0	36.0	36.0	32.0	36.0
30	34.10751051768919	36.0	36.0	36.0	32.0	36.0
31	34.04191047080986	36.0	36.0	36.0	32.0	36.0
32	33.947891550172095	36.0	36.0	36.0	32.0	36.0
33	33.865709661796615	36.0	36.0	36.0	32.0	36.0
34	33.75189283935382	36.0	36.0	36.0	27.0	36.0
35	33.54616321936944	36.0	36.0	36.0	21.0	36.0
36	33.444640158010934	36.0	36.0	36.0	21.0	36.0
37	33.22222011571613	36.0	36.0	36.0	14.0	36.0
38	33.07264401176809	36.0	36.0	36.0	14.0	36.0
39	32.59546580307577	36.0	36.0	36.0	14.0	36.0
40	32.38421752011761	36.0	36.0	36.0	14.0	36.0
41	32.44153267851634	36.0	36.0	36.0	14.0	36.0
42	32.035488691222014	36.0	36.0	36.0	14.0	36.0
43	32.136608452413164	36.0	36.0	36.0	14.0	36.0
44	32.07877279550786	36.0	36.0	36.0	14.0	36.0
45	31.595350328241526	36.0	32.0	36.0	14.0	36.0
46	31.812153467778213	36.0	36.0	36.0	14.0	36.0
47	31.54528250832022	36.0	32.0	36.0	14.0	36.0
48	31.54169244742879	36.0	32.0	36.0	14.0	36.0
49	31.629654971536315	36.0	36.0	36.0	14.0	36.0
50	30.967577424152854	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	5.0
21	30.0
22	130.0
23	393.0
24	1201.0
25	2810.0
26	5977.0
27	11343.0
28	18043.0
29	23798.0
30	29262.0
31	36239.0
32	49923.0
33	52441.0
34	84866.0
35	263749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4593837535014	18.487739711269125	14.27347554406378	22.779400991165698
2	9.83138078847536	23.10133182116677	43.2530770463802	23.814210343977678
3	19.85643129211837	29.986901294358937	29.14737767360094	21.00928973992175
4	10.845863236196426	19.084748333367806	36.16833157535522	33.901056855080554
5	9.543047122349895	41.484075675657046	35.86493236104672	13.107944840946342
6	29.79128246669309	35.21466365626239	20.678202719704935	14.315851157339585
7	24.422582741165744	33.103015616678704	23.996187606965027	18.478214035190526
8	26.734147631990318	36.93453955702475	22.25406876440204	14.077244046582893
9	26.805500738521886	16.20008514114644	20.01816574258074	36.976248377750935
10	15.76024666803398	29.755624227654327	34.955266428018675	19.528862676293016
11	32.73332379660573	21.914538281631057	24.283323538079983	21.068814383683236
12	22.793181124862766	25.540965128323563	33.69710778627849	17.96874596053518
13	33.24089601577352	22.697009546494133	25.977356591458655	18.084737846273697
14	22.746646490168267	22.652198416788316	26.959409734011473	27.641745359031944
15	24.1457878399829	31.034809630256476	29.095004765491296	15.72439776426933
16	21.228583296134353	26.063187139895177	34.15917947374499	18.549050090225485
17	19.411147285565818	28.000234396678465	33.66815290246858	18.920465415287143
18	22.051039876734922	22.789561764386526	38.880376689250326	16.27902166962822
19	21.74770299872633	31.02446860032436	28.37733728820278	18.850491112746525
20	21.490900755412238	24.908438797476098	37.1975464182981	16.403114028813555
21	20.484029141022347	28.01660769407097	26.059912480416674	25.439450684490005
22	19.793937743552796	32.00307473289982	27.894238839874326	20.308748683673063
23	17.76692352636015	30.51982633963734	28.882324249887542	22.830925884114972
24	19.907516722307154	31.736620861649083	29.52467455917051	18.831187856873253
25	23.37693226453044	27.111767575011246	28.196541614889703	21.31475854556861
26	15.65218290524342	28.305811831172345	32.911534212435775	23.130471051148458
27	21.42437346284899	31.055836391118437	31.092202346379693	16.427587799652887
28	19.241382044180327	26.94958575557597	29.713226004932668	24.09580619531103
29	18.164880828247558	29.348187648329148	32.295208828481954	20.19172269494134
30	22.148590259094505	26.33963734008028	32.17732108725589	19.334451313569325
31	18.964759493496352	26.811533005982284	31.034464929258736	23.18924257126262
32	17.272277594607495	31.10064752082425	31.440005653096364	20.18706923147189
33	17.13456954601155	30.292151330632027	32.212652939523934	20.36062618383249
34	16.503422019155035	31.872605405256344	34.48388781361328	17.140084761975345
35	20.446284381770145	30.733368607735436	31.142356341550432	17.67799066894399
36	18.537674957300162	30.782488499912965	32.30037934344801	18.379457199338862
37	20.49454252145333	30.555330542404256	29.677377101168016	19.272749834974398
38	19.97059700489303	32.53891243388204	26.36428346141848	21.12620709980645
39	19.804623474482646	28.931788843062805	31.221982272027688	20.04160541042686
40	23.271970810719512	29.053985346760587	28.34958885788495	19.324454984634954
41	19.190193946016375	27.90440751930757	32.42671225911863	20.478686275557425
42	22.67167402316046	31.540658344435577	29.991572060605332	15.796095571798633
43	19.52937972778962	29.333020804428717	28.83837487267607	22.299224595105592
44	18.661939666984367	29.729599302325184	33.080782402324665	18.52767862836579
45	20.555209897055047	29.935902849470796	29.780270348992527	19.72861690448163
46	19.117733234518536	27.423346719291292	32.431188707536926	21.027731338653247
47	23.607192531018782	26.512504890445403	31.614769058949044	18.26553351958677
48	17.61904679833096	35.219307392285245	26.128507978966347	21.033137830417452
49	18.84049478381215	27.864939255066673	34.30257508880359	18.99199087231758
50	16.785732136301668	36.45109640769855	24.505655681620368	22.257515774379407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20.0
1	29.5
2	39.0
3	176.0
4	313.0
5	340.5
6	368.0
7	366.5
8	365.0
9	471.5
10	578.0
11	833.5
12	1089.0
13	1941.5
14	2794.0
15	4046.0
16	5298.0
17	6474.0
18	7650.0
19	8279.0
20	8908.0
21	9550.0
22	10192.0
23	10148.0
24	10104.0
25	9500.0
26	8896.0
27	11318.5
28	13741.0
29	16139.5
30	18538.0
31	21373.5
32	24209.0
33	30286.0
34	36363.0
35	42011.5
36	47660.0
37	47516.5
38	47373.0
39	41595.0
40	35817.0
41	33693.5
42	31570.0
43	31869.5
44	32169.0
45	40685.0
46	49201.0
47	55988.5
48	62776.0
49	53407.0
50	44038.0
51	33204.5
52	22371.0
53	17161.0
54	11951.0
55	10955.5
56	9960.0
57	9141.5
58	8323.0
59	7622.0
60	6921.0
61	6205.0
62	5489.0
63	4840.0
64	4191.0
65	3845.0
66	3499.0
67	3010.5
68	2522.0
69	2201.5
70	1881.0
71	1540.5
72	1200.0
73	1008.0
74	816.0
75	652.0
76	488.0
77	368.5
78	249.0
79	193.5
80	138.0
81	102.5
82	67.0
83	53.0
84	39.0
85	28.0
86	17.0
87	12.0
88	7.0
89	3.5
90	0.0
91	1.5
92	3.0
93	3.0
94	3.0
95	3.0
96	3.0
97	3.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01516684390042967
2	0.005170514966055569
3	5.170514966055568E-4
4	1.7235049886851897E-4
5	0.0
6	5.170514966055568E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.170514966055568E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	580213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.313932899518306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.48030916140961	32.36182669990301
2	12.024434590489804	10.17602236833265
3	4.354630968361771	5.527846877922733
4	2.0802550011168828	3.5209508212458864
5	1.1749448331113534	2.485826841445477
6	0.7343981808184433	1.8645165206807954
7	0.5076282108410599	1.5035822236001797
8	0.3765142499636937	1.2745438966940958
9	0.2796299886401536	1.0649020118411272
>10	1.65619669781261	13.33431173988886
>50	0.18765422995017123	5.491697509517565
>100	0.12496624480790386	10.457214251334257
>500	0.010652860213132788	3.2612805226030592
>1k	0.006965331677817592	4.896341742563159
>5k	8.194507856255991E-4	2.779135972427145
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	8402	1.4480888914932963	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	7628	1.3146896053690629	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	3030	0.5222220115716125	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2729	0.47034451141218825	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2185	0.37658584002771395	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1900	0.32746594785018607	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1875	0.3231571853784731	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1815	0.31281615544636193	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1754	0.3023027750153823	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1686	0.29058294109232297	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1679	0.2893764876002433	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1463	0.25214877984464323	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1381	0.23801603893742468	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1195	0.20595884614788015	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	1157	0.19940952719087646	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.19854777469653387	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1089	0.18768969326781715	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1083	0.18665559027460604	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	1069	0.1842426832904468	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	996	0.1716610968730449	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	990	0.1706269938798338	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	966	0.16649058190698932	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	942	0.16235416993414487	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	926	0.15959656195224858	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	925	0.15942421145338004	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	845	0.14563617154389855	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	828	0.14270621306313372	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	824	0.14201681106765965	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	775	0.1335716366231022	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	767	0.13219283263215406	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	764	0.1316757811355485	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	711	0.12254120469551699	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	689	0.11874949372040956	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	637	0.10978726777924658	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	620	0.10685730929848178	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	606	0.1044444023143225	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	589	0.10151444383355768	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	586	0.10099739233695212	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	584	0.10065269133921508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.7235049886851897E-4	0.0
10	0.0	0.0	0.0	1.7235049886851897E-4	0.0
11	0.0	0.0	0.0	1.7235049886851897E-4	0.0
12	0.0	0.0	0.0	1.7235049886851897E-4	0.0
13	0.0	0.0	0.0	1.7235049886851897E-4	0.0
14	0.0	0.0	0.0	3.4470099773703793E-4	0.0
15	0.0	0.0	0.0	5.17051496605557E-4	0.0
16	0.0	0.0	0.0	8.617524943425949E-4	0.0
17	0.0	0.0	0.0	8.617524943425949E-4	0.0
18	0.0	0.0	0.0	8.617524943425949E-4	0.0
19	0.0	0.0	0.0	0.001034102993211114	0.0
20	0.0	0.0	0.0	0.0013788039909481517	0.0
21	0.0	0.0	0.0	0.0032746594785018606	0.0
22	0.0	0.0	0.0	0.006204617959266683	0.0
23	0.0	0.0	0.0	0.012064534920796328	0.0
24	0.0	0.0	0.0	0.023784368843855618	0.0
25	0.0	0.0	0.0	0.031195440295201934	0.0
26	0.0	0.0	0.0	0.03929591374202233	0.0
27	0.0	0.0	0.0	0.05377335564697792	0.0
28	0.0	0.0	0.0	0.07531716800554279	0.0
29	0.0	0.0	0.0	0.1044444023143225	0.0
30	0.0	0.0	0.0	0.15477074798393003	0.0
31	0.0	0.0	0.0	0.23181142097815802	0.0
32	0.0	0.0	0.0	0.34194338975514166	0.0
33	0.0	0.0	0.0	0.42742923719392706	0.0
34	0.0	0.0	0.0	0.5484192873996274	0.0
35	0.0	0.0	0.0	0.6607918126619018	0.0
36	0.0	0.0	0.0	0.8283164975621022	0.0
37	0.0	0.0	0.0	1.1135565731895012	0.0
38	0.0	0.0	0.0	1.3610518895646944	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	530	0.0	34.452763	4
TCGAATA	35	3.2158522E-4	31.42851	11
TCCTATA	80	1.3096724E-10	30.249939	2
GTACTAA	30	0.005740219	29.335804	1
ATACATG	185	0.0	28.542946	1
GTGTGCT	55	4.955291E-6	27.999943	9
TACACCG	40	7.0277764E-4	27.499945	5
CCGAGGG	50	8.334202E-5	26.399948	15
TCGTATA	125	0.0	26.399948	44
CCCCGAG	60	9.741721E-6	25.666618	13
TCCCCGA	60	9.741721E-6	25.666618	12
AGGACCT	1060	0.0	25.320704	5
TGGTATA	445	0.0	25.213432	44
CGCGGTA	150	0.0	24.933285	43
AAGTACT	395	0.0	24.50628	4
GCAAGAT	45	0.0013973541	24.444399	20
AGATAAC	45	0.0013973541	24.444399	23
TGTGCAA	45	0.0013973541	24.444399	10
ACGGGCC	45	0.0013973541	24.444399	30
GACGGGC	45	0.0013973541	24.444399	29
>>END_MODULE