FastQCFastQC Report
Thu 2 Feb 2017
SRR4062431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295854
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30160.2327422688049734No Hit
TATCAACGCAGAGTACTTTTTTTTT20840.16082058626974952No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18910.1459269331267257No Hit
GATTAAGAGGGACGGCCGGGGGCAT14900.11498208903163473No Hit
GAATAACGCCGCCGCATCGCCAGTC14830.11444190472074786No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14560.11235833666446993No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14320.11050627617000064No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13890.10718800111740984No Hit
GAACTACGACGGTATCTGATCGTCT13610.10502726387386233No Hit
GTATCTGATCGTCTTCGAACCTCCG13220.10201766557034973No Hit
GCGCAAGACGGACCAGAGCGAAAGC13180.10170898882127152No Hit
GAATAGGACCGCGGTTCTATTTTGT13120.10124597369765422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCA402.759557E-416.625889
TCGACGC551.1266275E-515.54747814
AATCGTA551.1266275E-515.54747813
CTCGACG508.702481E-515.2019813
CGAACGA1101.8553692E-1013.82158216
AGAACCG1750.013.5695265
ACTGAAC852.7088026E-713.4072943
GTACCGT500.001499150313.3001896
AACCGCG1750.013.0287587
GTATAGA957.4489435E-812.9911531
CGGTCCA2850.012.66733610
CGTTATT1352.7284841E-1112.6600012
TAGAACC2050.012.5089914
CGCGGTC1750.012.48637510
CGCGTAA1001.4406942E-712.35065310
CGACCAT1405.2750693E-1112.21493210
TAGTACG701.0960017E-412.2078582
TACCGTG550.003067642512.0910817
GCGTTAT1650.012.0826811
GCGTAAC1052.7178066E-711.76207211
Produced by FastQC (version 0.11.5)