FastQCFastQC Report
Thu 2 Feb 2017
SRR4062431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295854
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14930.1152135965934434No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14310.11042910698273109No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14180.10942590754822688No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14070.10857704648826179No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13580.10479575631205368No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13140.10140031207219331No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG13000.10031994345041956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA2900.022.0049781
GTTATAT1851.8189894E-1219.0313341
TATGGCG4300.018.93013216
AATCGTA1404.081812E-918.85704213
TAGGACG2950.018.643974
GTATCAA30950.018.4145051
TAGGCGT600.007412609618.33323726
CCTAATA1102.7968817E-617.9999072
TAAGACT1102.7968817E-617.9999074
CGCAATA4700.017.7871436
TGTAGGA4000.017.5999092
ATACGAA4500.017.59990740
GATATAT2150.017.3992861
ATATGGC6200.017.03216715
TTAACGG1451.2495002E-716.68956835
GTGTTAG1206.393484E-616.5037331
TAATACC1206.407574E-616.4999124
TCCTATA800.001988524616.4999122
TGGCGAG4700.016.38289318
CGAGCCG5650.016.35389715
Produced by FastQC (version 0.11.5)