##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1295854
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.299020568675175	32.0	32.0	32.0	32.0	32.0
2	31.430322397430576	32.0	32.0	32.0	32.0	32.0
3	31.509840614760613	32.0	32.0	32.0	32.0	32.0
4	31.607164078669356	32.0	32.0	32.0	32.0	32.0
5	31.555125037234134	32.0	32.0	32.0	32.0	32.0
6	35.13558703372448	36.0	36.0	36.0	36.0	36.0
7	35.14479177438199	36.0	36.0	36.0	36.0	36.0
8	35.09049784929475	36.0	36.0	36.0	36.0	36.0
9	35.21066339263528	36.0	36.0	36.0	36.0	36.0
10	35.088484505198885	36.0	36.0	36.0	36.0	36.0
11	35.20888541456059	36.0	36.0	36.0	36.0	36.0
12	35.12248061895862	36.0	36.0	36.0	36.0	36.0
13	35.17185037820619	36.0	36.0	36.0	36.0	36.0
14	35.13082646656182	36.0	36.0	36.0	36.0	36.0
15	35.091452432141274	36.0	36.0	36.0	36.0	36.0
16	35.10370381231219	36.0	36.0	36.0	36.0	36.0
17	35.07549924605704	36.0	36.0	36.0	36.0	36.0
18	35.09238309253975	36.0	36.0	36.0	36.0	36.0
19	35.06808483054418	36.0	36.0	36.0	36.0	36.0
20	35.05005810839801	36.0	36.0	36.0	36.0	36.0
21	35.05351837475518	36.0	36.0	36.0	36.0	36.0
22	35.03450697377945	36.0	36.0	36.0	36.0	36.0
23	35.00460082694501	36.0	36.0	36.0	36.0	36.0
24	34.97484901848511	36.0	36.0	36.0	36.0	36.0
25	34.9543251014389	36.0	36.0	36.0	32.0	36.0
26	34.91482142278374	36.0	36.0	36.0	32.0	36.0
27	34.891863589571045	36.0	36.0	36.0	32.0	36.0
28	34.88120575311725	36.0	36.0	36.0	32.0	36.0
29	34.851413816679965	36.0	36.0	36.0	32.0	36.0
30	34.83506706774065	36.0	36.0	36.0	32.0	36.0
31	34.84013708334427	36.0	36.0	36.0	32.0	36.0
32	34.80050530383824	36.0	36.0	36.0	32.0	36.0
33	34.77827208929401	36.0	36.0	36.0	32.0	36.0
34	34.76218385713205	36.0	36.0	36.0	32.0	36.0
35	34.735937073157935	36.0	36.0	36.0	32.0	36.0
36	34.7026617196073	36.0	36.0	36.0	32.0	36.0
37	34.69102846462641	36.0	36.0	36.0	32.0	36.0
38	34.66101813938916	36.0	36.0	36.0	32.0	36.0
39	34.651534817965604	36.0	36.0	36.0	32.0	36.0
40	34.64241650679784	36.0	36.0	36.0	32.0	36.0
41	34.60262884553352	36.0	36.0	36.0	32.0	36.0
42	34.582817971777686	36.0	36.0	36.0	32.0	36.0
43	34.567611783426216	36.0	36.0	36.0	32.0	36.0
44	34.51754595810948	36.0	36.0	36.0	32.0	36.0
45	34.4856511613191	36.0	36.0	36.0	32.0	36.0
46	34.47863879727191	36.0	36.0	36.0	32.0	36.0
47	34.447969447175375	36.0	36.0	36.0	32.0	36.0
48	34.398040983011974	36.0	36.0	36.0	32.0	36.0
49	34.38245435056727	36.0	36.0	36.0	32.0	36.0
50	33.872883056270226	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	16.0
21	31.0
22	110.0
23	361.0
24	830.0
25	2126.0
26	4282.0
27	8158.0
28	13327.0
29	20650.0
30	29269.0
31	41368.0
32	60978.0
33	99275.0
34	217736.0
35	797334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79504863458115	18.01914511497025	11.553597600647688	26.632208649800916
2	16.952809737945064	20.45335804913887	35.88756572209999	26.706266490816077
3	18.685607086606275	23.895611616441062	28.325975245649005	29.09280605130366
4	12.831259149392949	15.646088940781	35.24942296606631	36.27322894375974
5	14.947903081674324	36.1084659228586	32.74767064808227	16.195960347384812
6	34.3750723465597	35.03124599682369	16.57249395375685	14.021187702859752
7	30.506291603838086	30.22254050224794	20.494438416673482	18.77672947724049
8	28.549821198993097	31.85914462586063	19.449721959418266	20.141312215728004
9	28.092593764420993	13.803869880403195	18.528939216917955	39.57459713825786
10	16.633046624079565	25.989116057827506	30.52658710008998	26.851250218002953
11	37.90149198906667	20.88198207514118	21.872062747809554	19.344463187982598
12	25.19982961043451	23.580511384770197	27.89480913744913	23.324849867346167
13	29.808759320108592	18.94989713347337	25.10660923221289	26.13473431420515
14	24.106342226824935	19.51153447842118	24.44781588049271	31.934307414261177
15	25.834314668164776	26.498124017057478	21.920679335789373	25.746881978988373
16	26.40590683827036	25.199212256936356	23.257481166859847	25.137399737933443
17	24.57946651397457	25.140100659487874	24.827256774297105	25.45317605224045
18	25.532120130817205	23.876378048761666	26.04151393598353	24.5499878844376
19	26.32287279276832	24.316936938883547	25.05814698260761	24.302043285740524
20	26.29856449877841	23.57364332710321	24.64521466152823	25.482577512590154
21	27.564910861871784	23.439137433692377	23.871439220776413	25.124512483659423
22	26.58787178185197	23.874989003390816	24.294480705388107	25.242658509369107
23	24.939460772587037	23.567083946185296	25.166106675597717	26.327348605629957
24	25.2353274365785	24.623144273969135	24.766370285541427	25.375158003910936
25	25.311107578477205	23.872596758585455	25.058532828543957	25.757762834393382
26	24.868156443549967	24.605935545208023	25.532583145940823	24.993324865301183
27	25.896898879040386	24.29008206171374	24.470657959924498	25.342361099321376
28	24.850330361290702	24.119075142724412	25.426398344257915	25.60419615172697
29	24.938226065590722	24.426285677244504	25.15468563588182	25.480802621282955
30	25.026121769890743	24.531621617867444	25.348380295928397	25.093876316313413
31	25.507734667640026	24.24918239246088	24.332293607150188	25.910789332748905
32	25.278156335513106	24.306441929414888	24.41324408459595	26.002157650476054
33	25.044410867273626	23.85669990600793	25.02604460070347	26.07284462601497
34	25.441137659026403	24.209980445327947	25.291660943285276	25.057220952360375
35	26.332441771989746	23.944364102746142	24.943627908699593	24.77956621656452
36	24.87471582446788	24.450285294485337	24.823629822495434	25.85136905855135
37	26.028935358458593	24.34433200036424	24.3512772272185	25.27545541395867
38	25.10290511122395	24.08990518993652	25.115406519561617	25.69178317927791
39	25.590460036392987	24.014433724786898	24.455764306781475	25.93934193203864
40	25.914879299674194	24.203729741159112	25.035382072363088	24.846008886803606
41	24.73666014844265	24.62229541290917	25.402244388642547	25.238800050005633
42	26.05640758912655	24.71713634406345	24.8467805786763	24.379675488133696
43	25.030057398441492	23.69094049175293	25.240806448874643	26.038195660930942
44	24.797546637198327	24.390402005164162	24.895551505030657	25.91649985260685
45	24.929428778241995	24.557318957228205	24.93228403817097	25.580968226358834
46	24.71674962379905	24.146621908399894	25.081915345140253	26.0547131226608
47	25.046533091741956	23.891540083281114	25.420881396949653	25.641045428027276
48	25.632440074267627	24.797392298823787	24.436857855900435	25.133309771008154
49	24.66273206703842	24.607556098140684	24.941775848205122	25.78793598661578
50	24.327759398635493	25.17654394441345	24.74292994652943	25.752766710421632
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	25.5
2	34.0
3	82.5
4	131.0
5	154.0
6	177.0
7	192.0
8	207.0
9	285.5
10	364.0
11	513.5
12	663.0
13	1224.5
14	1786.0
15	2487.5
16	3189.0
17	3832.5
18	4476.0
19	4711.0
20	4946.0
21	5495.0
22	6044.0
23	6189.0
24	6334.0
25	7290.5
26	8247.0
27	10362.0
28	12477.0
29	14253.5
30	16030.0
31	17190.0
32	18350.0
33	21648.5
34	24947.0
35	28930.0
36	32913.0
37	40043.5
38	47174.0
39	50493.0
40	53812.0
41	58904.5
42	63997.0
43	64500.0
44	65003.0
45	72064.5
46	79126.0
47	84159.5
48	89193.0
49	91565.0
50	93937.0
51	90692.5
52	87448.0
53	86270.5
54	85093.0
55	87332.0
56	89571.0
57	88933.0
58	88295.0
59	81232.0
60	74169.0
61	65875.0
62	57581.0
63	51519.0
64	45457.0
65	39388.5
66	33320.0
67	30880.5
68	28441.0
69	26548.5
70	24656.0
71	20093.5
72	15531.0
73	13792.5
74	12054.0
75	9226.5
76	6399.0
77	5637.0
78	4875.0
79	4259.5
80	3644.0
81	2911.0
82	2178.0
83	1920.5
84	1663.0
85	1399.5
86	1136.0
87	827.5
88	519.0
89	346.5
90	174.0
91	113.5
92	53.0
93	33.0
94	13.0
95	9.5
96	6.0
97	3.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013195931023093653
2	0.00439864367436455
3	7.716918726955352E-5
4	2.315075618086605E-4
5	0.0
6	6.173534981564282E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.086767490782141E-4
47	1.5433837453910704E-4
48	0.0
49	0.0
50	7.716918726955352E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1295854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.3384025923579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05873317623103	41.100874748355096
2	11.862182632289969	12.179710152011566
3	3.207534198623484	4.940090460530656
4	1.355419210761211	2.7834022849390023
5	0.7640156818703833	1.9611672331368286
6	0.4961742942991136	1.528367740602418
7	0.347773678853557	1.2497901595206506
8	0.26555981193135414	1.0906733229826167
9	0.21058935281672952	0.9730188878912426
>10	1.1886719718304963	11.388635755735747
>50	0.12337853973673234	4.422278002660886
>100	0.10801609706415476	11.569634815366086
>500	0.009379543403712467	3.2018689913423173
>1k	0.002571810288114709	1.6104874449249087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1493	0.1152135965934434	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1431	0.11042910698273109	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1418	0.10942590754822688	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1407	0.10857704648826179	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1358	0.10479575631205368	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1314	0.10140031207219331	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1300	0.10031994345041956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.716918726955351E-5
2	0.0	0.0	0.0	0.0	7.716918726955351E-5
3	0.0	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
4	0.0	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
5	0.0	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
6	0.0	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
7	0.0	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
8	7.716918726955351E-5	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
9	7.716918726955351E-5	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
10	7.716918726955351E-5	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
11	7.716918726955351E-5	0.0	0.0	7.716918726955351E-5	7.716918726955351E-5
12	7.716918726955351E-5	0.0	0.0	1.5433837453910702E-4	1.5433837453910702E-4
13	7.716918726955351E-5	0.0	0.0	1.5433837453910702E-4	1.5433837453910702E-4
14	7.716918726955351E-5	0.0	0.0	2.3150756180866054E-4	1.5433837453910702E-4
15	7.716918726955351E-5	0.0	0.0	3.0867674907821403E-4	2.3150756180866054E-4
16	7.716918726955351E-5	0.0	0.0	3.8584593634776755E-4	2.3150756180866054E-4
17	7.716918726955351E-5	0.0	0.0	5.401843108868746E-4	3.0867674907821403E-4
18	7.716918726955351E-5	0.0	0.0	5.401843108868746E-4	3.0867674907821403E-4
19	7.716918726955351E-5	0.0	0.0	6.173534981564281E-4	3.8584593634776755E-4
20	7.716918726955351E-5	0.0	0.0	0.0010803686217737492	3.8584593634776755E-4
21	7.716918726955351E-5	0.0	0.0	0.0011575378090433028	3.8584593634776755E-4
22	1.5433837453910702E-4	0.0	0.0	0.0013890453708519633	3.8584593634776755E-4
23	1.5433837453910702E-4	0.0	0.0	0.0021607372435474984	3.8584593634776755E-4
24	1.5433837453910702E-4	0.0	0.0	0.0035497826143994615	3.8584593634776755E-4
25	1.5433837453910702E-4	0.0	0.0	0.00416713611255589	3.8584593634776755E-4
26	1.5433837453910702E-4	0.0	0.0	0.00571051985794696	4.630151236173211E-4
27	1.5433837453910702E-4	0.0	0.0	0.0072539036033380305	4.630151236173211E-4
28	1.5433837453910702E-4	0.0	0.0	0.014121961270328293	4.630151236173211E-4
29	1.5433837453910702E-4	0.0	0.0	0.028475430102465248	4.630151236173211E-4
30	1.5433837453910702E-4	0.0	0.0	0.05424993865049612	4.630151236173211E-4
31	1.5433837453910702E-4	0.0	0.0	0.11938073270599929	4.630151236173211E-4
32	1.5433837453910702E-4	0.0	0.0	0.19485219785562263	4.630151236173211E-4
33	1.5433837453910702E-4	0.0	0.0	0.27078667812886326	4.630151236173211E-4
34	1.5433837453910702E-4	0.0	0.0	0.35629013762352857	6.945226854259816E-4
35	1.5433837453910702E-4	0.0	0.0	0.4464237483543671	6.945226854259816E-4
36	1.5433837453910702E-4	0.0	0.0	0.5846337627541375	6.945226854259816E-4
37	1.5433837453910702E-4	0.0	0.0	0.7813380211042293	6.945226854259816E-4
38	1.5433837453910702E-4	0.0	0.0	1.0191734562689934	6.945226854259816E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	290	0.0	22.004978	1
GTTATAT	185	1.8189894E-12	19.031334	1
TATGGCG	430	0.0	18.930132	16
AATCGTA	140	4.081812E-9	18.857042	13
TAGGACG	295	0.0	18.64397	4
GTATCAA	3095	0.0	18.414505	1
TAGGCGT	60	0.0074126096	18.333237	26
CCTAATA	110	2.7968817E-6	17.999907	2
TAAGACT	110	2.7968817E-6	17.999907	4
CGCAATA	470	0.0	17.78714	36
TGTAGGA	400	0.0	17.599909	2
ATACGAA	450	0.0	17.599907	40
GATATAT	215	0.0	17.399286	1
ATATGGC	620	0.0	17.032167	15
TTAACGG	145	1.2495002E-7	16.689568	35
GTGTTAG	120	6.393484E-6	16.503733	1
TAATACC	120	6.407574E-6	16.499912	4
TCCTATA	80	0.0019885246	16.499912	2
TGGCGAG	470	0.0	16.382893	18
CGAGCCG	565	0.0	16.353897	15
>>END_MODULE