FastQCFastQC Report
Thu 2 Feb 2017
SRR4062430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706612
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40620.2380154364319482No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28770.1685796185659072No Hit
TATCAACGCAGAGTACTTTTTTTTT27310.16002465703979582No Hit
GTACATGGAAGCAGTGGTATCAACG25880.15164548239435793No Hit
TCCATGTACTCTGCGTTGATACCAC22800.1335980293118764No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20350.1192421007235388No Hit
GATTAAGAGGGACGGCCGGGGGCAT18700.1095738222864951No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18460.10816752724110694No Hit
GCGCAAGACGGACCAGAGCGAAAGC17850.10459319400074533No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17390.10189779516375135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA653.365134E-614.61850113
AACCGTC400.00527705614.2509467
TTAATCG400.005279110714.2501111
CGCAAGA4100.012.5045862
TCGCCAG2700.012.31816317
CGCATCG2800.012.21688913
AAGACGG4750.012.1986625
CGAACGA1251.4060788E-912.16294916
CGCCAGT2850.012.00291118
GCTCGTA1353.7107384E-1011.9637589
CGATAAC1353.7107384E-1011.96305610
GCATCGC2950.011.91744414
AAATCGT802.8639342E-511.87613712
GCGCAAG4650.011.8408681
CAAGACG4850.011.75064
CGCGTAT658.019647E-411.6930857
ATCGCCA2850.011.66949716
CGTCAAT2550.011.55148119
CGTTATT1900.011.4930072
GTATTAG3900.011.4404131
Produced by FastQC (version 0.11.5)