##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062430_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1706612
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17288053757972	32.0	32.0	32.0	32.0	32.0
2	30.860618582313965	32.0	32.0	32.0	32.0	32.0
3	30.847274600202038	32.0	32.0	32.0	32.0	32.0
4	30.913520472140124	32.0	32.0	32.0	32.0	32.0
5	30.739875847585743	32.0	32.0	32.0	32.0	32.0
6	34.39265691322925	36.0	36.0	36.0	32.0	36.0
7	34.271805190635014	36.0	36.0	36.0	32.0	36.0
8	34.26662475126157	36.0	36.0	36.0	32.0	36.0
9	34.40367406299733	36.0	36.0	36.0	32.0	36.0
10	34.09293207829313	36.0	36.0	36.0	32.0	36.0
11	34.400979250116606	36.0	36.0	36.0	32.0	36.0
12	34.187002083660495	36.0	36.0	36.0	32.0	36.0
13	34.306577007544774	36.0	36.0	36.0	32.0	36.0
14	34.214071505415404	36.0	36.0	36.0	32.0	36.0
15	34.16577933355678	36.0	36.0	36.0	32.0	36.0
16	34.165317014060605	36.0	36.0	36.0	32.0	36.0
17	34.08765319826651	36.0	36.0	36.0	32.0	36.0
18	34.104113295816504	36.0	36.0	36.0	32.0	36.0
19	34.08875889774594	36.0	36.0	36.0	32.0	36.0
20	34.08143678820962	36.0	36.0	36.0	32.0	36.0
21	34.057491099324274	36.0	36.0	36.0	32.0	36.0
22	33.99698584095272	36.0	36.0	36.0	32.0	36.0
23	33.97755318725053	36.0	36.0	36.0	32.0	36.0
24	33.954224510316344	36.0	36.0	36.0	32.0	36.0
25	33.523025151586886	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	10.0
4	60.0
5	150.0
6	291.0
7	69.0
8	237.0
9	328.0
10	221.0
11	69.0
12	152.0
13	96.0
14	314.0
15	393.0
16	595.0
17	726.0
18	1065.0
19	1494.0
20	2197.0
21	3353.0
22	4848.0
23	7156.0
24	10225.0
25	14222.0
26	19544.0
27	25203.0
28	34196.0
29	45325.0
30	59188.0
31	80204.0
32	115273.0
33	167457.0
34	380388.0
35	731561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.923294916819565	17.832708554672188	11.23810483741007	26.005891691098178
2	16.668610153203385	20.327794885016893	37.32074266577989	25.682852295999837
3	18.99901260756571	24.362575189787375	28.490754549476566	28.14765765317035
4	12.519024381095933	15.62287742994896	36.2742863036763	35.583811885278806
5	14.469685202064461	36.74170508465635	33.68633921741602	15.102270495863165
6	33.47475089736743	35.737119267356576	17.30472622306066	13.483403612215335
7	29.656284447780095	30.901329830898046	21.201010859112976	18.24137486220888
8	27.77662110686564	32.949149162651715	20.02433896257371	19.24989076790893
9	27.563926603179965	14.375538147154945	19.04249104358904	39.01804420607605
10	15.899535198055611	26.73147964005568	31.84048781031528	25.52849735157342
11	36.97700150069285	21.296345185169542	22.684808740861204	19.041844573276403
12	24.770304689897397	24.03458734054519	28.80052666073274	22.394581308824677
13	29.426227921294295	19.809659805916834	25.408984427938076	25.3551278448508
14	23.245384505267307	20.287407977583726	25.348494199977	31.118713317171963
15	24.975522820240414	27.686814118331693	22.6416112642544	24.696051797173492
16	25.332146787526955	25.912787709982236	24.435139276386955	24.319926226103853
17	23.58700058727692	26.053446306403664	25.816658130323848	24.54289497599557
18	24.47262978972079	24.732141757018645	27.151417576053788	23.643810877206775
19	25.256715438835105	25.321139997852516	25.821281109933057	23.600863453379326
20	25.407391241088316	24.49334234475249	25.691990162534083	24.407276251625106
21	26.408103419040618	24.471959384334475	24.66012308381939	24.459814112805518
22	25.463513684019023	25.054181497011434	25.07888814227741	24.403416676692128
23	23.91845845379475	24.80156506441984	25.866221964299026	25.413754517486392
24	24.375227004815585	25.533619009786655	25.72062110277839	24.370532882619365
25	24.421704087338306	25.017411217459212	25.814542811059393	24.74634188414309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	311.0
1	311.0
2	741.0
3	1171.0
4	1171.0
5	1171.0
6	3016.5
7	4862.0
8	4862.0
9	4862.0
10	4979.0
11	5096.0
12	5096.0
13	5096.0
14	5620.0
15	6144.0
16	6144.0
17	6144.0
18	9713.0
19	13282.0
20	13282.0
21	13282.0
22	21229.0
23	29176.0
24	29176.0
25	29176.0
26	43124.5
27	57073.0
28	57073.0
29	57073.0
30	70207.5
31	83342.0
32	83342.0
33	83342.0
34	100823.0
35	118304.0
36	118304.0
37	118304.0
38	129924.5
39	141545.0
40	141545.0
41	141545.0
42	159129.5
43	176714.0
44	176714.0
45	176714.0
46	200637.5
47	224561.0
48	224561.0
49	224561.0
50	222781.0
51	221001.0
52	221001.0
53	221001.0
54	201824.5
55	182648.0
56	182648.0
57	182648.0
58	167107.0
59	151566.0
60	151566.0
61	151566.0
62	134683.5
63	117801.0
64	117801.0
65	117801.0
66	98078.0
67	78355.0
68	78355.0
69	78355.0
70	59812.0
71	41269.0
72	41269.0
73	41269.0
74	32828.5
75	24388.0
76	24388.0
77	24388.0
78	20211.5
79	16035.0
80	16035.0
81	16035.0
82	11373.0
83	6711.0
84	6711.0
85	6711.0
86	4982.5
87	3254.0
88	3254.0
89	3254.0
90	2178.0
91	1102.0
92	1102.0
93	1102.0
94	665.5
95	229.0
96	229.0
97	229.0
98	450.5
99	672.0
100	672.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008261983391655514
2	0.00333995073279691
3	0.005683775808443864
4	0.014590311095902291
5	0.0349815892540308
6	0.04623194961713618
7	0.0663302496408088
8	0.08947552226282247
9	0.09890941819230147
10	0.11807018818571532
11	0.12105856515716518
12	0.13412538995389695
13	0.1221718820680975
14	0.12516025903954736
15	0.11502321558737429
16	0.11390989867644198
17	0.1251016634126562
18	0.13236752114716174
19	0.13435977246146166
20	0.1235195814865945
21	0.1315471823706853
22	0.153344755574202
23	0.14068810016570843
24	0.1378755100749321
25	0.13189875613203236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1706612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.089302461464094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00725631378279	40.63343568317587
2	13.701230727572348	14.273751029256434
3	4.029994707084278	6.29758839646237
4	1.5400440530990596	3.20879281943424
5	0.7346190182383839	1.9132896117481493
6	0.42115108904038095	1.316247987539964
7	0.2789503804722608	1.0171231518112056
8	0.19660360758827206	0.8192755824544401
9	0.13186545390982068	0.6181901561634212
>10	0.7500889809549153	7.30703902631666
>50	0.09065777613811658	3.2918545489027062
>100	0.0993773668247855	10.910257145037997
>500	0.013761391838228209	4.7937130775455845
>1k	0.004399133456482788	3.599441784150907
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4062	0.2380154364319482	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2877	0.1685796185659072	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2731	0.16002465703979582	No Hit
GTACATGGAAGCAGTGGTATCAACG	2588	0.15164548239435793	No Hit
TCCATGTACTCTGCGTTGATACCAC	2280	0.1335980293118764	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2035	0.1192421007235388	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1870	0.1095738222864951	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1846	0.10816752724110694	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1785	0.10459319400074533	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1739	0.10189779516375135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1719125378234771E-4
2	0.0	0.0	0.0	0.0	1.1719125378234771E-4
3	0.0	0.0	0.0	0.0	1.1719125378234771E-4
4	0.0	0.0	0.0	1.1719125378234771E-4	1.1719125378234771E-4
5	0.0	0.0	0.0	1.1719125378234771E-4	1.1719125378234771E-4
6	0.0	0.0	0.0	1.1719125378234771E-4	1.1719125378234771E-4
7	0.0	0.0	0.0	1.7578688067352156E-4	1.1719125378234771E-4
8	0.0	0.0	0.0	1.7578688067352156E-4	1.7578688067352156E-4
9	0.0	0.0	0.0	2.3438250756469543E-4	1.7578688067352156E-4
10	0.0	0.0	0.0	5.273606420205647E-4	1.7578688067352156E-4
11	0.0	0.0	0.0	5.273606420205647E-4	1.7578688067352156E-4
12	0.0	0.0	0.0	5.273606420205647E-4	5.859562689117386E-4
13	0.0	0.0	0.0	5.273606420205647E-4	5.859562689117386E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	65	3.365134E-6	14.618501	13
AACCGTC	40	0.005277056	14.250946	7
TTAATCG	40	0.0052791107	14.25011	11
CGCAAGA	410	0.0	12.504586	2
TCGCCAG	270	0.0	12.318163	17
CGCATCG	280	0.0	12.216889	13
AAGACGG	475	0.0	12.198662	5
CGAACGA	125	1.4060788E-9	12.162949	16
CGCCAGT	285	0.0	12.002911	18
GCTCGTA	135	3.7107384E-10	11.963758	9
CGATAAC	135	3.7107384E-10	11.963056	10
GCATCGC	295	0.0	11.917444	14
AAATCGT	80	2.8639342E-5	11.876137	12
GCGCAAG	465	0.0	11.840868	1
CAAGACG	485	0.0	11.7506	4
CGCGTAT	65	8.019647E-4	11.693085	7
ATCGCCA	285	0.0	11.669497	16
CGTCAAT	255	0.0	11.551481	19
CGTTATT	190	0.0	11.493007	2
GTATTAG	390	0.0	11.440413	1
>>END_MODULE