FastQCFastQC Report
Thu 2 Feb 2017
SRR4062430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706612
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19400.11367551616887728No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18770.10998399167473333No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17810.10435881149318064No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17640.10336268583603068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG550.00448378620.0004395
TAGGACG7700.019.714724
GTCCTAA5150.019.6532211
CTAGCGG5800.019.34468529
TAGCGGC5950.018.85700430
TGTAGGA8800.018.7504122
CGGTCCA5950.018.48725910
CGCAATA5500.018.39986436
TCTAGCG6400.017.53112228
AGGACGT8550.017.497465
GGACGTG8500.017.0827276
GTAGGAC10150.016.6900223
GCCGGTC6950.016.4603128
CGCCGGT6700.016.418277
TATGGCG9050.016.0440816
ATACGAA6450.016.0308940
ATTTCGT4400.015.99988242
AGCGGCG7300.015.97248531
CAATACG6250.015.83988438
CCGGTCC7500.015.8398839
Produced by FastQC (version 0.11.5)