##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062430_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1706612
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297924191321755	32.0	32.0	32.0	32.0	32.0
2	31.468639620487842	32.0	32.0	32.0	32.0	32.0
3	31.539792290221797	32.0	32.0	32.0	32.0	32.0
4	31.627897260771633	32.0	32.0	32.0	32.0	32.0
5	31.578361103754105	32.0	32.0	32.0	32.0	32.0
6	35.17657616376775	36.0	36.0	36.0	36.0	36.0
7	35.187767342547694	36.0	36.0	36.0	36.0	36.0
8	35.135719191005336	36.0	36.0	36.0	36.0	36.0
9	35.25499937888635	36.0	36.0	36.0	36.0	36.0
10	35.13620319088346	36.0	36.0	36.0	36.0	36.0
11	35.25844245792248	36.0	36.0	36.0	36.0	36.0
12	35.17420304087865	36.0	36.0	36.0	36.0	36.0
13	35.21365371859567	36.0	36.0	36.0	36.0	36.0
14	35.181857387619445	36.0	36.0	36.0	36.0	36.0
15	35.138614986886296	36.0	36.0	36.0	36.0	36.0
16	35.15377543343185	36.0	36.0	36.0	36.0	36.0
17	35.12857579813103	36.0	36.0	36.0	36.0	36.0
18	35.137128415832066	36.0	36.0	36.0	36.0	36.0
19	35.122604903750826	36.0	36.0	36.0	36.0	36.0
20	35.1054873632671	36.0	36.0	36.0	36.0	36.0
21	35.09966940347308	36.0	36.0	36.0	36.0	36.0
22	35.085311717015934	36.0	36.0	36.0	36.0	36.0
23	35.0549480491172	36.0	36.0	36.0	36.0	36.0
24	35.0334252894038	36.0	36.0	36.0	36.0	36.0
25	35.00610332049698	36.0	36.0	36.0	36.0	36.0
26	34.96674580982672	36.0	36.0	36.0	36.0	36.0
27	34.9458435778021	36.0	36.0	36.0	32.0	36.0
28	34.934486573398054	36.0	36.0	36.0	32.0	36.0
29	34.90499832416507	36.0	36.0	36.0	32.0	36.0
30	34.88914351944086	36.0	36.0	36.0	32.0	36.0
31	34.889882410295954	36.0	36.0	36.0	32.0	36.0
32	34.843781128926786	36.0	36.0	36.0	32.0	36.0
33	34.832636826648354	36.0	36.0	36.0	32.0	36.0
34	34.81535287458426	36.0	36.0	36.0	32.0	36.0
35	34.787422097114046	36.0	36.0	36.0	32.0	36.0
36	34.75920244320326	36.0	36.0	36.0	32.0	36.0
37	34.73614564997785	36.0	36.0	36.0	32.0	36.0
38	34.70833733736784	36.0	36.0	36.0	32.0	36.0
39	34.69062856700879	36.0	36.0	36.0	32.0	36.0
40	34.67645428486381	36.0	36.0	36.0	32.0	36.0
41	34.64052520432295	36.0	36.0	36.0	32.0	36.0
42	34.61025646133978	36.0	36.0	36.0	32.0	36.0
43	34.60211108324564	36.0	36.0	36.0	32.0	36.0
44	34.56237680269446	36.0	36.0	36.0	32.0	36.0
45	34.51564444642367	36.0	36.0	36.0	32.0	36.0
46	34.51334808380581	36.0	36.0	36.0	32.0	36.0
47	34.480982203336204	36.0	36.0	36.0	32.0	36.0
48	34.431781799260754	36.0	36.0	36.0	32.0	36.0
49	34.419523008158855	36.0	36.0	36.0	32.0	36.0
50	33.92153108029242	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	11.0
21	49.0
22	133.0
23	398.0
24	1063.0
25	2469.0
26	5297.0
27	9831.0
28	16580.0
29	25475.0
30	36945.0
31	52790.0
32	76957.0
33	125848.0
34	280004.0
35	1072761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47110615195642	18.429146077319057	11.724852084876558	26.37489568584797
2	16.131423431553227	20.8986578144731	36.92272984304545	26.04718891092822
3	18.426946145678784	24.624576058652153	28.89621728297836	28.0522605126907
4	12.231082537262445	16.102146300475034	36.553789938070246	35.112981224192275
5	14.119972272533108	37.084256459146225	33.60150614287614	15.194265125444522
6	33.60353825077376	36.003037607977475	16.952126032605065	13.441298108643707
7	29.62570805615112	30.944170574513553	21.22179128318203	18.208330086153303
8	27.810422052581373	33.045296763411955	19.93095091327144	19.213330270735234
9	27.68297656409307	14.138245834436885	18.995647516834524	39.18313008463553
10	15.888965974691377	26.82531237328696	31.818187145056992	25.46753450696468
11	37.07374611218015	21.24419610315643	22.674105186181745	19.00795259848167
12	24.61156958933841	24.037273850178014	28.928660996172535	22.422495564311042
13	29.53289910067432	19.64676212284925	25.430209092634996	25.390129683841433
14	23.367115665423658	20.2638326696402	25.33094810068135	31.038103564254794
15	24.98382760697804	27.702723290355397	22.65488582056144	24.65856328210513
16	25.437826524130852	25.782661788385408	24.41451249610339	24.36499919138035
17	23.64427298061891	25.99776633470291	25.760219663286087	24.597741021392093
18	24.56768146479692	24.621765228417473	27.172608653870945	23.637944652914662
19	25.351925335108387	25.16623579349026	25.85813295582124	23.62370591558011
20	25.46630399879996	24.44000159380105	25.680178036952743	24.413516370446242
21	26.557530358394292	24.34308442692305	24.695361335792786	24.404023878889873
22	25.465776638157937	24.995781114863835	25.12375396399416	24.414688282984066
23	23.96115813084638	24.578580251398677	26.02841184756699	25.431849770187952
24	24.49771828628886	25.379406684120347	25.707014834068904	24.415860195521887
25	24.55672408256827	24.782493032979964	25.867684043004502	24.793098841447264
26	23.989987179276838	25.6340046829625	26.267306218402307	24.108701919358356
27	24.951717203441675	25.275223659507844	25.33463962517549	24.43841951187499
28	24.03223462626537	24.940525438705457	26.11571933163484	24.911520603394326
29	24.13899585846109	25.20162755213253	25.952237532608468	24.707139056797914
30	24.125225886141667	25.231276939339466	26.3496916698113	24.29380550470757
31	24.711064963799622	24.996484262386527	25.2642076816523	25.028243092161546
32	24.224486878095313	25.307392658671095	25.3193461665569	25.14877429667669
33	23.893597373040855	24.900387434285005	26.05267043709994	25.153344755574203
34	24.536508591290815	25.115609171856285	26.286759966530177	24.06112227032272
35	25.323330669185495	24.972811629122496	25.902841419139204	23.8010162825528
36	24.126104820545034	25.32538151612669	25.74082451078511	24.807689152543166
37	25.131547182370685	25.2469805673463	25.09920239632676	24.52226985395626
38	24.134249612682908	25.15311037306664	25.825495191642855	24.8871448226076
39	24.747277061218366	24.894703658476562	25.365343733666467	24.992675546638605
40	25.025137523936316	24.975858601720834	26.028529038820775	23.970474835522076
41	24.073310160716087	25.234148125057132	26.30662388404629	24.3859178301805
42	25.17865806639119	25.71990587198496	25.687971255329273	23.413464806294577
43	24.17491497774538	24.66811436928839	26.03743557410823	25.119535078857997
44	23.821173178203363	25.28448176855665	25.82127630650669	25.073068746733295
45	23.92816879290665	25.4846444300169	25.855496152611142	24.731690624465315
46	23.84838949730753	24.93076918569562	26.04420459273062	25.17663672426624
47	24.283434078747835	24.672684828185904	26.37277834680642	24.67110274625984
48	24.561118754585106	25.78758382104427	25.340206209730155	24.311091214640467
49	23.68710638387636	25.494254112827054	25.81072909366628	25.007910409630306
50	23.516944683384388	26.147360970156075	25.435599890308985	24.900094456150548
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	64.5
2	60.0
3	190.5
4	321.0
5	351.5
6	382.0
7	350.0
8	318.0
9	424.5
10	531.0
11	837.0
12	1143.0
13	1900.0
14	2657.0
15	3604.5
16	4552.0
17	5289.0
18	6026.0
19	6603.5
20	7181.0
21	8073.5
22	8966.0
23	9787.0
24	10608.0
25	12277.0
26	13946.0
27	16900.5
28	19855.0
29	23330.5
30	26806.0
31	29782.0
32	32758.0
33	38188.0
34	43618.0
35	49950.5
36	56283.0
37	64788.0
38	73293.0
39	77023.0
40	80753.0
41	86795.5
42	92838.0
43	93849.5
44	94861.0
45	103188.5
46	111516.0
47	117266.0
48	123016.0
49	124842.0
50	126668.0
51	119551.5
52	112435.0
53	110082.0
54	107729.0
55	108932.0
56	110135.0
57	107209.0
58	104283.0
59	95090.0
60	85897.0
61	75814.0
62	65731.0
63	57865.0
64	49999.0
65	43042.0
66	36085.0
67	31905.5
68	27726.0
69	25931.0
70	24136.0
71	19135.0
72	14134.0
73	12614.5
74	11095.0
75	8237.5
76	5380.0
77	4906.5
78	4433.0
79	3840.0
80	3247.0
81	2613.0
82	1979.0
83	1748.0
84	1517.0
85	1247.0
86	977.0
87	718.5
88	460.0
89	296.5
90	133.0
91	87.0
92	41.0
93	28.5
94	16.0
95	10.5
96	5.0
97	8.0
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01335980293118764
2	0.003925907001708649
3	4.6876501512939085E-4
4	5.8595626891173856E-5
5	5.8595626891173856E-5
6	3.515737613470432E-4
7	1.757868806735216E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1719125378234771E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1706612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.855547198976964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.8718897362314	44.05432559834579
2	13.470413256290684	15.047946068529386
3	3.7709660066125132	6.318881093042486
4	1.404331701658128	3.1375886257994074
5	0.6749322088927315	1.8849353924958874
6	0.40540184708593224	1.3586365202676438
7	0.2562333982527205	1.0018439669041364
8	0.16645452999020144	0.7437927085080978
9	0.13482716372406983	0.6777760506384577
>10	0.6749896256590184	7.048679709744536
>50	0.07353252870211394	2.9020455309147373
>100	0.08298625062692994	9.838066483555052
>500	0.009886485078384775	3.6440905074009886
>1k	0.003155261195229183	2.3413917438534573
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1940	0.11367551616887728	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1877	0.10998399167473333	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1781	0.10435881149318064	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1764	0.10336268583603068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1719125378234771E-4
2	0.0	0.0	0.0	0.0	1.1719125378234771E-4
3	0.0	0.0	0.0	0.0	1.1719125378234771E-4
4	0.0	0.0	0.0	5.8595626891173856E-5	1.1719125378234771E-4
5	5.8595626891173856E-5	0.0	0.0	5.8595626891173856E-5	1.1719125378234771E-4
6	5.8595626891173856E-5	0.0	0.0	1.1719125378234771E-4	1.7578688067352156E-4
7	5.8595626891173856E-5	0.0	0.0	1.1719125378234771E-4	1.7578688067352156E-4
8	5.8595626891173856E-5	0.0	0.0	1.1719125378234771E-4	1.7578688067352156E-4
9	5.8595626891173856E-5	0.0	0.0	1.1719125378234771E-4	1.7578688067352156E-4
10	5.8595626891173856E-5	0.0	0.0	1.7578688067352156E-4	1.7578688067352156E-4
11	5.8595626891173856E-5	0.0	0.0	1.7578688067352156E-4	1.7578688067352156E-4
12	5.8595626891173856E-5	0.0	0.0	1.7578688067352156E-4	5.273606420205647E-4
13	5.8595626891173856E-5	0.0	0.0	1.7578688067352156E-4	5.859562689117386E-4
14	5.8595626891173856E-5	0.0	0.0	1.7578688067352156E-4	6.445518958029125E-4
15	5.8595626891173856E-5	0.0	0.0	3.515737613470431E-4	9.375300302587817E-4
16	5.8595626891173856E-5	0.0	0.0	3.515737613470431E-4	0.0010547212840411294
17	5.8595626891173856E-5	0.0	0.0	3.515737613470431E-4	0.0010547212840411294
18	5.8595626891173856E-5	0.0	0.0	4.6876501512939085E-4	0.0010547212840411294
19	5.8595626891173856E-5	0.0	0.0	4.6876501512939085E-4	0.0010547212840411294
20	5.8595626891173856E-5	0.0	0.0	5.859562689117386E-4	0.0010547212840411294
21	5.8595626891173856E-5	0.0	0.0	9.375300302587817E-4	0.0011133169109323034
22	5.8595626891173856E-5	0.0	0.0	0.0013476994184969987	0.0011133169109323034
23	5.8595626891173856E-5	0.0	0.0	0.002402420702538128	0.0011719125378234772
24	5.8595626891173856E-5	0.0	0.0	0.004570458897511561	0.001289103791605825
25	5.8595626891173856E-5	0.0	0.0	0.005449393300879169	0.001289103791605825
26	5.8595626891173856E-5	0.0	0.0	0.006855688346267342	0.001289103791605825
27	5.8595626891173856E-5	0.0	0.0	0.009492491556370166	0.001289103791605825
28	5.8595626891173856E-5	0.0	0.0	0.01611379739507281	0.001289103791605825
29	5.8595626891173856E-5	0.0	0.0	0.029415004699369276	0.001289103791605825
30	5.8595626891173856E-5	0.0	0.0	0.060529282578582595	0.001289103791605825
31	5.8595626891173856E-5	0.0	0.0	0.12557042842778557	0.0013476994184969987
32	5.8595626891173856E-5	0.0	0.0	0.2017447433863116	0.0013476994184969987
33	5.8595626891173856E-5	0.0	0.0	0.27405174697002016	0.0014062950453881725
34	5.8595626891173856E-5	0.0	0.0	0.3570817502748135	0.0016992731798440418
35	5.8595626891173856E-5	0.0	0.0	0.4541161084065974	0.0016992731798440418
36	5.8595626891173856E-5	0.0	0.0	0.5888860502562973	0.0016992731798440418
37	5.8595626891173856E-5	0.0	0.0	0.7893416898510031	0.0016992731798440418
38	5.8595626891173856E-5	0.0	0.0	1.0414786723637242	0.0016992731798440418
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	55	0.004483786	20.000439	5
TAGGACG	770	0.0	19.71472	4
GTCCTAA	515	0.0	19.653221	1
CTAGCGG	580	0.0	19.344685	29
TAGCGGC	595	0.0	18.857004	30
TGTAGGA	880	0.0	18.750412	2
CGGTCCA	595	0.0	18.487259	10
CGCAATA	550	0.0	18.399864	36
TCTAGCG	640	0.0	17.531122	28
AGGACGT	855	0.0	17.49746	5
GGACGTG	850	0.0	17.082727	6
GTAGGAC	1015	0.0	16.690022	3
GCCGGTC	695	0.0	16.460312	8
CGCCGGT	670	0.0	16.41827	7
TATGGCG	905	0.0	16.04408	16
ATACGAA	645	0.0	16.03089	40
ATTTCGT	440	0.0	15.999882	42
AGCGGCG	730	0.0	15.972485	31
CAATACG	625	0.0	15.839884	38
CCGGTCC	750	0.0	15.839883	9
>>END_MODULE