FastQCFastQC Report
Thu 2 Feb 2017
SRR4062429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464478
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG47220.3224357074670975No Hit
TCCATGTACTCTGCGTTGATACCAC39800.27176919011415673No Hit
GTATCAACGCAGAGTACTTTTTTTT22210.1516581334782769No Hit
GAGTACATGGAAGCAGTGGTATCAA20400.13929878086253258No Hit
CATGTACTCTGCGTTGATACCACTG16930.11560433137268024No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16690.1139655221860622No Hit
TATCAACGCAGAGTACTTTTTTTTT16640.11362410360551678No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16380.11184872698668058No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16060.10966364807118986No Hit
GAATAGGACCGCGGTTCTATTTTGT15180.10365468105359042No Hit
GAATAACGCCGCCGCATCGCCAGTC14660.10010392781591801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC851.024091E-915.6418523
GCGTTAT1100.015.5360341
CGTTATT1151.8189894E-1214.8646172
TACCTCG456.7615317E-414.777917
TCGCCAG2750.014.16763817
CGCCAGT2650.013.98651818
ATCGCCA2850.013.66959316
TCGCGTA1400.013.571559
CGCATCG2750.013.4737713
CGGAGTA500.001495127913.30512418
GCGGAGT500.001496284413.30375917
TCGCATG500.001499758613.29966511
CGCGTAA1450.013.10356710
CATCGCC3000.012.98433815
ATAAGGC905.426082E-712.6624513
CGGTCCA4150.012.59047510
GCATCGC3100.012.56548914
ATCCCCG2050.012.51230812
TAACCTG1001.4439502E-712.3488455
GCGTAAC1551.8189894E-1212.25775511
Produced by FastQC (version 0.11.5)