##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062429_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1464478
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.091735075569588	32.0	32.0	32.0	32.0	32.0
2	30.580666285188308	32.0	32.0	32.0	32.0	32.0
3	30.578233336383338	32.0	32.0	32.0	32.0	32.0
4	30.671850311168896	32.0	32.0	32.0	32.0	32.0
5	30.371478438050964	32.0	32.0	32.0	27.0	32.0
6	34.051277656612115	36.0	36.0	36.0	32.0	36.0
7	33.9253829692218	36.0	36.0	36.0	32.0	36.0
8	33.88827759788812	36.0	36.0	36.0	32.0	36.0
9	34.13680096252726	36.0	36.0	36.0	32.0	36.0
10	33.654575896667616	36.0	36.0	36.0	27.0	36.0
11	34.126172602114885	36.0	36.0	36.0	32.0	36.0
12	33.81271825182761	36.0	36.0	36.0	32.0	36.0
13	33.98876732870006	36.0	36.0	36.0	32.0	36.0
14	33.85318659617966	36.0	36.0	36.0	32.0	36.0
15	33.767686506728	36.0	36.0	36.0	32.0	36.0
16	33.771257062243336	36.0	36.0	36.0	32.0	36.0
17	33.665523824871386	36.0	36.0	36.0	27.0	36.0
18	33.691862219848986	36.0	36.0	36.0	27.0	36.0
19	33.68647736599662	36.0	36.0	36.0	27.0	36.0
20	33.686336018704274	36.0	36.0	36.0	27.0	36.0
21	33.65767666021613	36.0	36.0	36.0	27.0	36.0
22	33.60600227521342	36.0	36.0	36.0	27.0	36.0
23	33.54642131872244	36.0	36.0	36.0	27.0	36.0
24	33.522430517904674	36.0	36.0	36.0	27.0	36.0
25	33.01965410200768	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	9.0
4	67.0
5	136.0
6	248.0
7	71.0
8	220.0
9	254.0
10	184.0
11	57.0
12	96.0
13	86.0
14	190.0
15	296.0
16	417.0
17	661.0
18	912.0
19	1388.0
20	2272.0
21	3530.0
22	5418.0
23	8122.0
24	11428.0
25	16102.0
26	22258.0
27	28474.0
28	37932.0
29	49140.0
30	62317.0
31	83422.0
32	116782.0
33	160958.0
34	328222.0
35	522807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.17229993888136	16.980056201834927	10.846954140531903	26.00068971875181
2	16.907465703379472	19.613160069105387	36.81910367856436	26.660270548950788
3	19.135348486245267	23.62099282643804	28.0890190896644	29.154639597652288
4	12.546567986357587	14.938907582480399	35.507223117253325	37.00730131390869
5	14.662297670961891	36.495185634501674	33.644545722810584	15.19797097172585
6	35.041375159601614	34.79762618006136	16.6586851628056	13.502313497531427
7	30.490833890791112	30.196400519580262	20.84682563381071	18.465939955817912
8	28.498144815769848	32.83515480143822	19.376474938097875	19.29022544469405
9	27.208006709166256	14.200111682739003	18.330527595000387	40.261354013094355
10	15.561557940101945	26.83161602240161	31.875259784296993	25.73156625319945
11	37.744701593094106	20.959137953076088	21.76494837467211	19.531212079157697
12	24.218124483347818	23.630858895579678	28.99973402455042	23.15128259652208
13	29.7067236565403	19.196549432699168	25.144662395160783	25.952064515599748
14	23.50109968694644	19.46162171179246	24.959851342288072	32.07742725897303
15	25.51292945216202	26.952748213556937	22.340296809661865	25.194025524619178
16	26.217148326013024	25.555004870712487	23.654162322902604	24.57368448037188
17	24.29447710152775	25.772591842064603	25.28793155549357	24.644999500914082
18	25.22463882070602	24.510143104261676	26.259871322398244	24.005346752634065
19	25.402626172154097	25.625655944782693	25.018853605089618	23.95286427797359
20	25.304455132478065	24.26555091799726	25.405706589548743	25.024287359975933
21	26.446915548675847	24.57394191416073	24.1380041214445	24.841138415718923
22	25.413153341676413	24.608046283568925	24.694896361186906	25.28390401356776
23	24.08146659008233	24.328109187385465	25.342099504936954	26.24832471759525
24	24.500692637259096	25.448641596798964	24.927420093728248	25.12324567221369
25	25.109071329675402	24.542126044818215	24.72468078560074	25.624121839905644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	168.0
2	411.0
3	654.0
4	654.0
5	654.0
6	1795.0
7	2936.0
8	2936.0
9	2936.0
10	3116.5
11	3297.0
12	3297.0
13	3297.0
14	3679.5
15	4062.0
16	4062.0
17	4062.0
18	6617.0
19	9172.0
20	9172.0
21	9172.0
22	14687.0
23	20202.0
24	20202.0
25	20202.0
26	29516.5
27	38831.0
28	38831.0
29	38831.0
30	48166.0
31	57501.0
32	57501.0
33	57501.0
34	72673.5
35	87846.0
36	87846.0
37	87846.0
38	101129.0
39	114412.0
40	114412.0
41	114412.0
42	133281.0
43	152150.0
44	152150.0
45	152150.0
46	178491.5
47	204833.0
48	204833.0
49	204833.0
50	201995.5
51	199158.0
52	199158.0
53	199158.0
54	184590.0
55	170022.0
56	170022.0
57	170022.0
58	156333.0
59	142644.0
60	142644.0
61	142644.0
62	125932.0
63	109220.0
64	109220.0
65	109220.0
66	90105.0
67	70990.0
68	70990.0
69	70990.0
70	53375.0
71	35760.0
72	35760.0
73	35760.0
74	27716.0
75	19672.0
76	19672.0
77	19672.0
78	15948.0
79	12224.0
80	12224.0
81	12224.0
82	8461.0
83	4698.0
84	4698.0
85	4698.0
86	3480.0
87	2262.0
88	2262.0
89	2262.0
90	1572.5
91	883.0
92	883.0
93	883.0
94	541.0
95	199.0
96	199.0
97	199.0
98	440.5
99	682.0
100	682.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0077160599203265606
2	0.003277618373236061
3	0.004984711275963175
4	0.015090701260107696
5	0.035917234673378505
6	0.046228075805850276
7	0.06753259523188467
8	0.08747144033573737
9	0.09593862113326387
10	0.11785769400428003
11	0.11792597772038911
12	0.13226555810329688
13	0.12031590778420707
14	0.12215956811915235
15	0.11300955016053502
16	0.11287298272831683
17	0.12318382386078862
18	0.13028533033613343
19	0.12926107459449715
20	0.12174986582249785
21	0.12864652114951539
22	0.1494047708466771
23	0.13916221343031443
24	0.13328981384493316
25	0.129943911755588
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1464478.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.000044614112774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34478359388099	44.189666821746904
2	11.983937198766302	13.182341611697446
3	3.435727330890194	5.668954694426619
4	1.4477942496464087	3.185149932904336
5	0.7030804046320231	1.9334726811034864
6	0.4365426832660193	1.4405920233357339
7	0.29701301176728656	1.1435010228720934
8	0.20249962668384505	0.8909990801562127
9	0.15874271283436994	0.7857770659250075
>10	0.819320743361786	8.306868192894438
>50	0.07698078637299613	2.9642195725559475
>100	0.07941059406859433	9.151878424767352
>500	0.009941799178398799	3.6145490729107403
>1k	0.004225264650819489	3.542029802703712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4722	0.3224357074670975	No Hit
TCCATGTACTCTGCGTTGATACCAC	3980	0.27176919011415673	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2221	0.1516581334782769	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2040	0.13929878086253258	No Hit
CATGTACTCTGCGTTGATACCACTG	1693	0.11560433137268024	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1669	0.1139655221860622	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1664	0.11362410360551678	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1638	0.11184872698668058	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1606	0.10966364807118986	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1518	0.10365468105359042	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1466	0.10010392781591801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.82837161090846E-5	0.0
5	0.0	0.0	0.0	6.82837161090846E-5	0.0
6	6.82837161090846E-5	0.0	0.0	6.82837161090846E-5	6.82837161090846E-5
7	6.82837161090846E-5	0.0	0.0	6.82837161090846E-5	6.82837161090846E-5
8	6.82837161090846E-5	0.0	0.0	6.82837161090846E-5	6.82837161090846E-5
9	6.82837161090846E-5	0.0	0.0	6.82837161090846E-5	6.82837161090846E-5
10	1.365674322181692E-4	0.0	0.0	6.82837161090846E-5	6.82837161090846E-5
11	1.365674322181692E-4	0.0	0.0	6.82837161090846E-5	6.82837161090846E-5
12	1.365674322181692E-4	0.0	0.0	6.82837161090846E-5	5.462697288726768E-4
13	1.365674322181692E-4	0.0	0.0	1.365674322181692E-4	6.82837161090846E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	85	1.024091E-9	15.641852	3
GCGTTAT	110	0.0	15.536034	1
CGTTATT	115	1.8189894E-12	14.864617	2
TACCTCG	45	6.7615317E-4	14.77791	7
TCGCCAG	275	0.0	14.167638	17
CGCCAGT	265	0.0	13.986518	18
ATCGCCA	285	0.0	13.669593	16
TCGCGTA	140	0.0	13.57155	9
CGCATCG	275	0.0	13.47377	13
CGGAGTA	50	0.0014951279	13.305124	18
GCGGAGT	50	0.0014962844	13.303759	17
TCGCATG	50	0.0014997586	13.299665	11
CGCGTAA	145	0.0	13.103567	10
CATCGCC	300	0.0	12.984338	15
ATAAGGC	90	5.426082E-7	12.662451	3
CGGTCCA	415	0.0	12.590475	10
GCATCGC	310	0.0	12.565489	14
ATCCCCG	205	0.0	12.512308	12
TAACCTG	100	1.4439502E-7	12.348845	5
GCGTAAC	155	1.8189894E-12	12.257755	11
>>END_MODULE