FastQCFastQC Report
Thu 2 Feb 2017
SRR4062429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464478
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24350.166270848725621No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21100.1440786409901685No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16880.11526291279213481No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16090.10986849921951712No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15710.1072737180073719No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15490.10577147625297205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1451.2732926E-1121.2470191
TAACGGC1900.020.84194436
CGATTAA550.004484258619.99984633
ACGATTA550.004484258619.99984632
ACCGTCG3000.019.7998478
GTATTAG4500.019.5607471
TTAACGG2050.019.31692335
GTATAAT1851.8189894E-1219.0320781
CGCAATA5100.018.11750636
CAACGGA1751.9099389E-1017.59986314
TATACAG2950.017.1524095
CAATACG5650.016.74323338
TACCGTC3550.016.7322647
TAATACC1854.2200554E-1016.648524
ATACCGT3700.016.648526
GTACCGT800.00198872416.4998726
TCGGATT800.00198872416.49987236
CGTCGTA3350.016.41778210
TATTAGA3500.016.3438472
CGAATGC5950.016.26878243
Produced by FastQC (version 0.11.5)