##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1464478
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288794369051633	32.0	32.0	32.0	32.0	32.0
2	31.396243576209407	32.0	32.0	32.0	32.0	32.0
3	31.493376479537417	32.0	32.0	32.0	32.0	32.0
4	31.5973753105202	32.0	32.0	32.0	32.0	32.0
5	31.53554167423478	32.0	32.0	32.0	32.0	32.0
6	35.12627844187485	36.0	36.0	36.0	36.0	36.0
7	35.13977540120097	36.0	36.0	36.0	36.0	36.0
8	35.07659452719672	36.0	36.0	36.0	36.0	36.0
9	35.22097976207222	36.0	36.0	36.0	36.0	36.0
10	35.088434923570034	36.0	36.0	36.0	36.0	36.0
11	35.22153968854431	36.0	36.0	36.0	36.0	36.0
12	35.130357711075206	36.0	36.0	36.0	36.0	36.0
13	35.18264460101142	36.0	36.0	36.0	36.0	36.0
14	35.14154599795968	36.0	36.0	36.0	36.0	36.0
15	35.100846171810026	36.0	36.0	36.0	36.0	36.0
16	35.11191769353995	36.0	36.0	36.0	36.0	36.0
17	35.08670393136667	36.0	36.0	36.0	36.0	36.0
18	35.10137195642406	36.0	36.0	36.0	36.0	36.0
19	35.07989809338208	36.0	36.0	36.0	36.0	36.0
20	35.073383826865275	36.0	36.0	36.0	36.0	36.0
21	35.070181320579756	36.0	36.0	36.0	36.0	36.0
22	35.05364437021246	36.0	36.0	36.0	36.0	36.0
23	35.007137014007725	36.0	36.0	36.0	36.0	36.0
24	34.987372975217106	36.0	36.0	36.0	36.0	36.0
25	34.957677752755586	36.0	36.0	36.0	32.0	36.0
26	34.9043672899149	36.0	36.0	36.0	32.0	36.0
27	34.892378717877634	36.0	36.0	36.0	32.0	36.0
28	34.87126675853103	36.0	36.0	36.0	32.0	36.0
29	34.85112442795317	36.0	36.0	36.0	32.0	36.0
30	34.833475135850456	36.0	36.0	36.0	32.0	36.0
31	34.828393461697615	36.0	36.0	36.0	32.0	36.0
32	34.795818032090615	36.0	36.0	36.0	32.0	36.0
33	34.77499013300302	36.0	36.0	36.0	32.0	36.0
34	34.757900084535244	36.0	36.0	36.0	32.0	36.0
35	34.720621272562646	36.0	36.0	36.0	32.0	36.0
36	34.69364305916511	36.0	36.0	36.0	32.0	36.0
37	34.67465677190098	36.0	36.0	36.0	32.0	36.0
38	34.63173635930345	36.0	36.0	36.0	32.0	36.0
39	34.60971349518395	36.0	36.0	36.0	32.0	36.0
40	34.56989316329778	36.0	36.0	36.0	32.0	36.0
41	34.55350370575727	36.0	36.0	36.0	32.0	36.0
42	34.49660220228641	36.0	36.0	36.0	32.0	36.0
43	34.50107478569156	36.0	36.0	36.0	32.0	36.0
44	34.473981172813794	36.0	36.0	36.0	32.0	36.0
45	34.40940867667524	36.0	36.0	36.0	32.0	36.0
46	34.44299129109485	36.0	36.0	36.0	32.0	36.0
47	34.39670722264179	36.0	36.0	36.0	32.0	36.0
48	34.36977749068269	36.0	36.0	36.0	32.0	36.0
49	34.38036624653972	36.0	36.0	36.0	32.0	36.0
50	33.845424786169545	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	14.0
21	35.0
22	103.0
23	366.0
24	939.0
25	2189.0
26	4530.0
27	8600.0
28	14660.0
29	22699.0
30	33262.0
31	48309.0
32	72329.0
33	115986.0
34	254858.0
35	885596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81110846281346	17.381285884338293	11.401308380450645	26.406297272397598
2	16.2731859746833	20.04480988576279	36.46713452321039	27.214869616343524
3	18.540564163144346	23.808476786823903	28.53332604969716	29.117633000334592
4	12.334915468115922	15.483479767862521	35.708447452571804	36.473157311449754
5	14.35781213510889	36.61352372654284	33.5773565734685	15.451307564879773
6	35.21066651371041	35.05279380745676	16.373011141907217	13.363528536925621
7	30.579564868847463	30.25835826827033	20.75599633453012	18.40608052835208
8	28.476221561539333	32.990662884659244	19.24016612062455	19.292949433176872
9	27.259678875339883	13.964088228023911	18.283579541652383	40.49265335498383
10	15.51494798829344	26.812557102257596	31.980746723405883	25.691748186043085
11	38.000366000718344	20.797239699060004	21.741398641700318	19.46099565852133
12	24.159325029123004	23.58594666495502	28.93809261730118	23.316635688620792
13	29.770880818967576	19.09335613099002	25.18474159393313	25.951021456109274
14	23.35712793227348	19.33337339311345	24.956537414696566	32.352961259916505
15	25.439371571303905	26.958479403582707	22.29634040251885	25.305808622594533
16	26.270111261487028	25.41765734958122	23.66734085455705	24.644890534374706
17	24.377013516078765	25.709501952231445	25.31359296623097	24.59989156545882
18	25.36350836270671	24.4306162332244	26.21186525164598	23.994010152422913
19	25.47030409470132	25.55354194463829	25.04284803185845	23.933305928801936
20	25.494476530203936	24.05150504138676	25.41294577316969	25.041072655239617
21	26.501456491664605	24.451579334069887	24.194149724338637	24.852814449926868
22	25.470782080714084	24.491320456845376	24.751071712924333	25.28682574951621
23	24.086534587750723	24.18957471535933	25.283343279994646	26.440547416895306
24	24.619557275698234	25.2814996196597	24.95476203807773	25.14418106656433
25	25.169787460105237	24.26646217969816	24.83096366077196	25.732786699424643
26	24.570119865235256	25.054456263596997	25.462588034780993	24.912835836386755
27	25.614519303123707	24.779272887677383	24.663736840020814	24.942470969178096
28	24.656157347532705	24.298692093701646	25.501919455259824	25.54323110350582
29	24.537480248935115	24.91078732490348	25.64121823612236	24.910514190039045
30	24.86531036997483	24.85042451986305	25.68444182841941	24.59982328174271
31	24.970876995079475	24.738166090579718	24.671043197644487	25.619913716696324
32	24.779204603961276	24.86892940692861	24.620991233736525	25.73087475537359
33	24.380769120464766	24.44830171569665	25.27378355973938	25.897145604099208
34	25.026801358572815	24.760972851760148	25.617318935484178	24.594906854182856
35	25.942485991595643	24.39708892861484	25.235954381014942	24.42447069877458
36	24.723280240467936	25.179210612928294	24.975588571491002	25.12192057511277
37	25.91933781183466	24.737210118554188	24.810478545939237	24.532973523671917
38	24.69815183293979	24.64454911579416	25.16582700457091	25.491472046695137
39	25.11891609160397	24.644822250658596	24.84762488750258	25.388636770234857
40	25.63876002234243	24.748545215428297	24.990269570454455	24.622425191774816
41	24.241197204737798	25.085457070710522	25.664571267031665	25.008774457520015
42	26.12965165745064	25.182351663869312	24.836426358060688	23.851570320619363
43	25.176342696851712	23.95003543924866	25.123013114570515	25.750608749329114
44	24.74540416448728	24.73850750916026	25.23083310230676	25.2852552240457
45	24.89153131696072	24.9628195165786	25.051520063804304	25.09412910265637
46	24.52636675072398	24.657198440125725	25.50302940913377	25.313405400016524
47	25.01075468528718	24.391148245313346	25.712506435740245	24.88559063365923
48	25.466958192611976	25.618548042374144	24.437717739699742	24.47677602531414
49	24.508527953304863	25.101845162576698	25.265111527793522	25.124515356324917
50	24.448643134277194	25.705473212981005	24.57435345563402	25.271530197107772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	20.5
2	28.0
3	94.0
4	160.0
5	191.0
6	222.0
7	196.0
8	170.0
9	238.5
10	307.0
11	443.5
12	580.0
13	1097.0
14	1614.0
15	2060.5
16	2507.0
17	2987.0
18	3467.0
19	3868.0
20	4269.0
21	4854.5
22	5440.0
23	6164.5
24	6889.0
25	8072.5
26	9256.0
27	11300.5
28	13345.0
29	15432.5
30	17520.0
31	19182.0
32	20844.0
33	24237.5
34	27631.0
35	31697.5
36	35764.0
37	43554.0
38	51344.0
39	56272.0
40	61200.0
41	67694.0
42	74188.0
43	77116.0
44	80044.0
45	90794.5
46	101545.0
47	109448.5
48	117352.0
49	118506.0
50	119660.0
51	113268.0
52	106876.0
53	104829.0
54	102782.0
55	103884.0
56	104986.0
57	101654.0
58	98322.0
59	90025.5
60	81729.0
61	72105.0
62	62481.0
63	54144.5
64	45808.0
65	39038.5
66	32269.0
67	27771.0
68	23273.0
69	21368.0
70	19463.0
71	15036.5
72	10610.0
73	9532.0
74	8454.0
75	6092.5
76	3731.0
77	3330.5
78	2930.0
79	2549.5
80	2169.0
81	1711.0
82	1253.0
83	1112.5
84	972.0
85	794.5
86	617.0
87	445.5
88	274.0
89	173.5
90	73.0
91	46.5
92	20.0
93	15.0
94	10.0
95	11.0
96	12.0
97	7.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015363836124544035
2	0.004711576411526837
3	5.462697288726769E-4
4	6.828371610908461E-5
5	0.0
6	4.779860127635922E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.828371610908461E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1464478.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.83026373961629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03748048603487	45.48551125075754
2	12.365353677792106	14.054526214851196
3	3.461976580002899	5.902351263058188
4	1.4042219317995157	3.192092109324799
5	0.6960917715546987	1.9779539482215132
6	0.4315218537327259	1.4714100454223367
7	0.29143278308735165	1.159354134765721
8	0.22080313438535112	1.0038640289322756
9	0.15345259220844637	0.7848676158060418
>10	0.780696311656982	8.111866757707542
>50	0.07328021863015226	2.8966432791300507
>100	0.07187103357813909	8.463688388440733
>500	0.00940586522323931	3.5913401896168926
>1k	0.002411760313651105	1.904530773965138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2435	0.166270848725621	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2110	0.1440786409901685	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1688	0.11526291279213481	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1609	0.10986849921951712	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1571	0.1072737180073719	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1549	0.10577147625297205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.365674322181692E-4	0.0	0.0	0.0	0.0
7	1.365674322181692E-4	0.0	0.0	6.82837161090846E-5	0.0
8	1.365674322181692E-4	0.0	0.0	6.82837161090846E-5	0.0
9	1.365674322181692E-4	0.0	0.0	1.365674322181692E-4	0.0
10	1.365674322181692E-4	0.0	0.0	1.365674322181692E-4	0.0
11	2.048511483272538E-4	0.0	0.0	1.365674322181692E-4	6.82837161090846E-5
12	2.048511483272538E-4	0.0	0.0	1.365674322181692E-4	1.365674322181692E-4
13	2.048511483272538E-4	0.0	0.0	2.048511483272538E-4	3.41418580545423E-4
14	2.048511483272538E-4	0.0	0.0	2.048511483272538E-4	4.097022966545076E-4
15	2.048511483272538E-4	0.0	0.0	2.048511483272538E-4	4.097022966545076E-4
16	2.731348644363384E-4	0.0	0.0	2.731348644363384E-4	4.779860127635922E-4
17	2.731348644363384E-4	0.0	0.0	3.41418580545423E-4	4.779860127635922E-4
18	2.731348644363384E-4	0.0	0.0	3.41418580545423E-4	4.779860127635922E-4
19	2.731348644363384E-4	0.0	0.0	4.097022966545076E-4	4.779860127635922E-4
20	2.731348644363384E-4	0.0	0.0	4.097022966545076E-4	6.145534449817614E-4
21	2.731348644363384E-4	0.0	0.0	4.779860127635922E-4	6.82837161090846E-4
22	2.731348644363384E-4	0.0	0.0	7.511208771999306E-4	6.82837161090846E-4
23	2.731348644363384E-4	0.0	0.0	0.0012291068899635228	7.511208771999306E-4
24	2.731348644363384E-4	0.0	0.0	0.002185078915490707	7.511208771999306E-4
25	2.731348644363384E-4	0.0	0.0	0.0030044835087997225	7.511208771999306E-4
26	2.731348644363384E-4	0.0	0.0	0.0036873206698905684	8.194045933090152E-4
27	2.731348644363384E-4	0.0	0.0	0.005735832153163107	8.876883094180998E-4
28	2.731348644363384E-4	0.0	0.0	0.008876883094180998	8.876883094180998E-4
29	2.731348644363384E-4	0.0	0.0	0.015158984976216781	8.876883094180998E-4
30	2.731348644363384E-4	0.0	0.0	0.02697206786308842	8.876883094180998E-4
31	2.731348644363384E-4	0.0	0.0	0.05107621964959528	9.559720255271845E-4
32	2.731348644363384E-4	0.0	0.0	0.08235016162755603	9.559720255271845E-4
33	2.731348644363384E-4	0.0	0.0	0.11335096874108044	9.559720255271845E-4
34	2.731348644363384E-4	0.0	0.0	0.1498827568594407	0.001024255741636269
35	2.731348644363384E-4	0.0	0.0	0.19057985166045513	0.001024255741636269
36	2.731348644363384E-4	0.0	0.0	0.24944041494648606	0.001024255741636269
37	2.731348644363384E-4	0.0	0.0	0.33773125987553243	0.001024255741636269
38	2.731348644363384E-4	0.0	0.0	0.4513553634810492	0.001024255741636269
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	145	1.2732926E-11	21.247019	1
TAACGGC	190	0.0	20.841944	36
CGATTAA	55	0.0044842586	19.999846	33
ACGATTA	55	0.0044842586	19.999846	32
ACCGTCG	300	0.0	19.799847	8
GTATTAG	450	0.0	19.560747	1
TTAACGG	205	0.0	19.316923	35
GTATAAT	185	1.8189894E-12	19.032078	1
CGCAATA	510	0.0	18.117506	36
CAACGGA	175	1.9099389E-10	17.599863	14
TATACAG	295	0.0	17.152409	5
CAATACG	565	0.0	16.743233	38
TACCGTC	355	0.0	16.732264	7
TAATACC	185	4.2200554E-10	16.64852	4
ATACCGT	370	0.0	16.64852	6
GTACCGT	80	0.001988724	16.499872	6
TCGGATT	80	0.001988724	16.499872	36
CGTCGTA	335	0.0	16.417782	10
TATTAGA	350	0.0	16.343847	2
CGAATGC	595	0.0	16.268782	43
>>END_MODULE