FastQCFastQC Report
Thu 2 Feb 2017
SRR4062428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506065
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32900.2184500668961831No Hit
TATCAACGCAGAGTACTTTTTTTTT20640.13704587783395802No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16630.11042020098734119No Hit
GCGCAAGACGGACCAGAGCGAAAGC15840.1051747434539678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGT456.752911E-414.78044116
CGAAATC456.7597267E-414.77847613
ATTCGCA701.0907126E-412.21445714
GAGTGTT2450.012.0221767
TTAGACT1052.7236456E-711.7605064
CGAACGA1302.6066118E-911.69441616
GCGTTAT1552.5465852E-1111.635651
CGACCAT2050.011.58668410
CCGACCA2050.011.5866849
CAAGACG3200.011.5767484
CTAACAC1406.8394E-1011.530893
GTATAAG1657.2759576E-1211.5057461
AACCGCG1751.8189894E-1211.4016777
AGAGTGT2500.011.4016766
GACCATA2250.011.40091811
GTGCTAA1001.9483086E-611.3906891
ATCGCCA2600.011.32896616
CGCAAGA3200.011.2746582
CGCATCG2550.011.17699913
CGCGTAA1451.2150849E-911.13919810
Produced by FastQC (version 0.11.5)